FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287226
Sequences flagged as poor quality0
Sequence length150
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG108723.7851726515009085No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC106023.6911700194272106No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG104453.636509229665838No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG94993.307151859511326No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC92153.208275016885658No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC90203.140384227054654No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT86753.020269752738262No Hit
GGAGTTGAACCCAGCCGTGACAGCCGCCTTGCTGCAACTTTTATCCCAGC82152.860117120316406No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA82062.856983699247283No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA81392.8336571201771426No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA79182.756714225035338No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA77612.7020534352739656No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT74332.5878576451992505No Hit
GCAGAGCCAAACCGACCTGAGCATCTCTCAAATGGCACAGCTGCTTAACA71962.505344223712338No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAAGGTAATTAAATGTGGT55251.923572378545118No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT54601.9009421152681165No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC53961.8786600098876842No Hit
CTGAGGGAAGCAGCAATTCTGTGGTACATGCAGAGACCAAATTGCAAAAC52481.827132641195435No Hit
CCAAGGAACATCTTCAGTATCTCTAGGATTCTCTGAGCATGGCAGTTTCT46951.6346013243926385No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT41321.4385884286241495No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA39721.3828831651730693No Hit
GCAAACAGAAGGTTCCTTGAGCTACGGTAGCAAATACTGAGCATCAAGTT38991.3574676387235138No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT38331.3344892175499434No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC35021.2192489537855207No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC30441.0597926371568034No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT29291.0197544790513393No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG28811.0030429000160153No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA27130.944552373392381No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA25110.8742244782853921No Hit
GTTTGAATCCATGCTTTGCTCTTACGGTAGCAGAGCTGTTGCCACCTGAA25010.8707428993196995No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT24340.8474163202495597No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT23720.825830530662266No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA23510.8185192148343117No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC23330.8122523726960651No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGCA20820.7248647406571829No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT20690.7203386880017826No Hit
ATATATGTCTTCCACCCTTGACTCTCTGCTACCGTATCCAAGGAACATCT20390.7098939511047051No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC19000.6615000034815789No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC18680.650358950791363No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG18650.6493144771016551No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA17320.6030094768579446No Hit
TGGTAAATTCATCTGCTTTCTCTGGATACGGTAGCAAATACTGAGCATCA16950.5901276346848823No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC15500.5396447396823407No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGCATGCC15130.5267628975092784No Hit
AGCAGGATTTTTGCTACTGATTTCTGCTACTACCCCTCCACCTCAGACAG15000.5222368448538781No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGGTACATGT14640.5097031605773851No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG14310.49821394999059976No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGC13370.46548710771309004No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC12850.44738289709148893No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC11960.41639684429682544No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG11740.4087373705723019No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGCATGC11590.4035150021237632No Hit
TATCAACTGACCCAGGCAAAATATAAATTACCTTGACAAGTTGATGAAGT11560.40247052843405545No Hit
GCACAAGCGGCATAAGTCCAAACACTCCAAAGACATGGGGTTGGTGACCC11070.38541079150216206No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGCAT10970.38192921253646955No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG10790.375662370398223No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA10410.36243237032859144No Hit
CCAAATAACAAGTGTTGGAAGCAGAGCTTGGTCTCTTACCAATACTCCAT9890.34432815970699027No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC6970.2426660539087687No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT6430.2238655274940291No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC6270.21829500114892106No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC6160.21446526428665927No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGCA6140.21376894849352077No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT6120.2130726327003823No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG5970.2078502642518435No Hit
TCAAATCATCCATTGCTTGGTACGGGTAGCAGAAAGTCAGTCCCATGGAG5340.1859163167679806No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT5080.17686421145718006No Hit
CGAAGGGAGAACGACGAACGTCGTGGATCAGATCGGAGCGACCGCCTGCA4860.1692047377326565No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT4680.16293789559441No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC4610.1605007903184252No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACTGTATGGCTGAGATC4590.1598044745252867No Hit
TTTTCCTGGCTTTGCTTACGACTACGGTAGCAGAAACTACCTGCTGCCAC4390.15284131659390165No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA4380.1524931586973324No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG4330.15075236921448615No Hit
GAGAAAGCCCCCCTACTGCTCGTACCATCCAAAAGACCTATATCCAGGTA4110.14309289548996262No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG3750.13055921121346953No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGCATG3710.12916657962719252No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTG3710.12916657962719252No Hit
TTCCCAATCACAGCCATTCGTCAGTGTACCCTCAATTTGGAAGCCTCTTA3700.12881842173062327No Hit
TCAACTTGAGGGAGGGAGCTTTACCTTTCTATCCTGGGATTCTCTTGCTC3660.12742579014434627No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC3230.11245500059186841No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC3200.11141052690216066No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT3110.10827710583303739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)