FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287226
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG147535.136373448086176No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG105953.6887329141512257No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG105743.6814215983232716No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG103613.6072639663540205No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT93873.2681581750955693No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA91473.184600279918949No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA83632.9116444890086552No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG81432.8350497517634197No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT81072.8225160674869265No Hit
GTGTCAATGGCACTGAACCCTGTTTCAGGCCCATCAGTGTTTCCATAAGT80612.806500804244741No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG78332.727120803826952No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC74852.6059618558208517No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC73582.561745802956557No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA53241.8535926413346984No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC52751.836532904402805No Hit
TGCACGTACCACATTTAATTACCTTTGGTCTTCTCTTGGTATTAGTGCTT50691.764812377709539No Hit
TCCCTCTTCCCCCTCTTGGTGGGACTCTTGTAGGCCCCCGATAGAGTTTT47231.6443497454965774No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA46241.6098821137362218No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA40741.418395270623133No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT39641.3800979020005153No Hit
AGTGAACTTGATGCTCAGTATTTGCTACCGTAGCTCAAGGAACCTTCTGT36481.2700800066846316No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT34851.2133302695438435No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT29101.0131394790165236No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC28150.9800644788424446No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCTTG27030.9410707944266884No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA24850.8651723729745915No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG24740.8613426361123296No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC24340.8474163202495597No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC24070.8380160570421898No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT23570.8206081622137271No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT23490.8178228990411731No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA23010.8011113200058491No Hit
ATTTTTCAGGTGGCAACAGCTCTGCTACCGTAAGAGCAAAGCATGGATTC22770.792755530488187No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA20970.7300871091057216No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT20540.7151163195532437No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG18990.6611518455850097No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA18530.6451365823428241No Hit
AGAGATACTGAAGATGTTCCTTGGATACGGTAGCAGAGAGTCAAGGGTGG18380.6399142138942854No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG18160.6322547401697618No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT17140.5967426347196981No Hit
AGTGAACTTGATGCTCAGTATTTGCTACCGTATCCAGAGAAAGCAGATGA15590.542778160751464No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC15040.5236294764401551No Hit
GTCTGAGGTGGAGGGGTAGTAGCAGAAATCAGTAGCAAAAATCCTGCTTG14960.5208442132676011No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC14940.5201478974744626No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT14210.49473237102490725No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT14110.4912507920592147No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG13180.4588721076782742No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG12670.44111605495324235No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG11960.41639684429682544No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT11630.4049076337100402No Hit
GCATGCCATTCAAAGGATTTTACAAACAATGACATGAACCAAGGAACTTC11520.4010778968477784No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG11490.4000334231580706No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT10990.38262552832960806No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA10830.3770550019845No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT10300.35860263346632965No Hit
TCGCTCCGATCTGATCCACGACGTTCGTCGTTCTCCCTTCGGTCTAGTTT9880.34398000181042104No Hit
ATTGGTGATGATTCGATGGAGTATTGGTAAGAGACCAAGCTCTGCTTCCA9570.33318710701677423No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGTGGTGG6610.23013236963227565No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT6370.2217765801146136No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG6180.21516158007979777No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG6170.21481342218322855No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA6070.211331843217536No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG6060.21098368532096679No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT5870.20436868528615096No Hit
CCATGGGACTGACTTTCTGCTACCCGTACCAAGCAATGGATGATTTGATG5350.18626447466454985No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC5070.1765160535606108No Hit
GAACGCTTTGAACTTCCCGATATCCGGTCCTTCATCGATCCCGACTTGCT4720.16433052718068697No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT4610.1605007903184252No Hit
CAGCCATATCAGTCTGTGGGATCTCAGCCATACAGTCTATGAAGCTGGTA4420.15388579028360944No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT4360.1517968429041939No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC4330.15075236921448615No Hit
GGTGGCAGCAGGTAGTTTCTGCTACCGTAGTCGTAAGCAAAGCCAGGAAA4300.1497078955247784No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT4270.14866342183507064No Hit
ACCTGGATATAGGTCTTTTGGATGGTACGAGCAGTAGGGGGGCTTTCTCT4050.1410039481105471No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4030.1403076323174086No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG4020.13995947442083934No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT3730.12986289542033103No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC3720.12951473752376177No Hit
CAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT3680.12812210593748477No Hit
CGCACGTACCACATTTAATTACCTTTGGTCTTCTCTTGGTATTAGTGCTT3680.12812210593748477No Hit
AAGAGGCTTCCAAATTGAGGGTACACTGACGAATGGCTGTGATTGGGAAT3630.12638131645463851No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAATGCTC3620.12603315855806926No Hit
ATATGACGTGTCTGCTCCACTTCCATTGAAGGAAGCTTCTCTTTCTCTTA3320.11558842166099169No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG3260.11349947428157618No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG3200.11141052690216066No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT3100.10792894793646814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)