FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445943
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT127962.869425016201622No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT115302.5855322316977727No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTTTTTAAGGATAAGCTGTTAAC112632.5256591088995677No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT103462.3200274474540468No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC99142.2231540802299845No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT89282.002049589297287No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC83491.8722123679483702No Hit
TCAAAGGGCTCCACTGGTTTTTCTGAGCAGGACTTCACTTTTTCAAGCTT82421.8482182700479657No Hit
CTTTCTTCAGAAGCTCCACCCTATAATTCTGAACCTGCAGAAGAATCTGA80871.8134604646782213No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC78811.7672662201223026No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA76231.7094112924745986No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAATTATTTTACCAGATGATTCA74631.673532267576798No Hit
CCTGCCCTGGAGGAAGACAGTACAGCATCACACCCACGCTGAGAGTCGTC73921.6576109502783987No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC73871.6564897307503423No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT73771.65424729169423No Hit
CAACTTGTATTCAGCAGGTACCGCACTTCTTGAACAAAACCTGCATACTT66061.4813552404679522No Hit
CGCCCGGCCTTCATTTTCTTAAATAACTATTTTGTTGTTTTTACTTTTTA63991.4349367520064222No Hit
TTTCCTCTAAAATGTTCCTCTGTTGACTTTTTTTTTCTTTTAGACATAAT63971.4344882641952No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA63851.4317973373278647No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA62691.4057850442769593No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC62601.4037668491264579No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA61081.369681775473547No Hit
AGAACAAGAAGCTATATGACTGAATTCTTTTCAGTTCATTAAAGTTTTCA58271.3066692379967844No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC56951.2770690424560986No Hit
TGGATCCTTTATTACGAATTGTGATCACATCCTCTGATTGGCAACATGTT56281.2620447007801445No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT54131.2138322610737247No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG53191.1927533339462666No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA50711.1371408453546754No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT50581.134225674581729No Hit
GAAAAACAATTTATATCTGTCAGTGAATCCACTAGGACTGCTCCCACCAG48211.0810798689518615No Hit
TGCCAAAATGACGAACACAAAGGAAAAGAGAAAAGGCATCTGTATATGTT46981.0534978685616772No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG46471.042061429375503No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA45361.017170355852654No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG45171.01290972164604No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC44510.9981096238756971No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT43420.9736670381640703No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA41000.9194000130061466No Hit
GTTACTCTGTTTGATTCTCGGCATTTACTCTACCAACTGCTCTGGAACAT36910.8276842556111431No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA34670.777453620754222No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC34210.7671384010961042No Hit
CATGTTATGTGTTTTTCACCACAATAAAAATATGAAGATTGTTACTAGTT33090.7420230836676436No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC32550.7299139127646358No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT32500.7287926932365796No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC30540.6848408877367735No Hit
TGTTAATAAAAATAAAACTTAACAATTTTCCCCTTTTTTTACCCCCAGTG30160.6763196193235458No Hit
GCAGGAAGGAAAAGGAACCTGTACACTGACTATTTTATTAAACTCTCACC29500.6615195215532029No Hit
AAATGTCCCAAAGCTAAATCCATACTTACTACTGAGCAGTGGCTAGCTGA28480.638646643180855No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA26920.6036645939054991No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC26360.5911069351912689No Hit
CGCCTCATGTGGTTTTATGCAGCAGATGCAAGGTATTCTGTAAAGGTTCT25970.5823614228724299No Hit
ACATGTACATAGGGTTTATATATCATCAGCTATATGACTTATTTAATTTC25290.5671128372908645No Hit
GACTACAGATGTGCCACCATACCCAGCTAATTTTTTAATTTTTTGTAGAG24640.552536983426133No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAGTCTCTGCTACCGTAAAG24540.5502945443700203No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA23850.5348217148828438No Hit
GCCCTCTCAGAAACATCTGCTACCGTACTTCGAGGGCTAAACCACAAGAC23510.5271974220920611No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA23260.5215913244517798No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT22790.5110518608880508No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA20990.47068795787802475No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA19380.4345846890746127No Hit
GGGAAAATTATGCTTACTAATGTGGTTTTAATTTCATCATGTTTCATATA18520.41529971319204473No Hit
TTTTAGGTGCTTTTGAATTGTGGAAGGTAGCACAGAGATTCCAGACCAAG18410.41283303023032086No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATCA18330.4110390789854309No Hit
TTCCATGAAGCAAACGCTGATGACTTGGTCCCACAGACTGATATGGCTGA17860.40049961542170187No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC16860.3780752248605764No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA16340.36641454176879107No Hit
TTGTTTTTATTGTGTGATACATGTTTACTTTAAATTGTTTTTCTTTTTTG15640.3507174683760032No Hit
TTGTATGAACATCTGACTTTGGAAAAATCTTCAAGCAATTTAGCAGTTTC15170.3401780048122742No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCAAA15150.3397295170010517No Hit
ACAGAGTTTGAATGGATATTGTGGGTTTTTTTGCTTTGCAGATTTTTAAA14920.3345719071719928No Hit
AAGAAGAACAAACAACAGGACCATTGTTTAAATTCAGTGCCTTCTGGATC14910.33434766326638155No Hit
TTTCCTCTAAAATGTTCCTCTGTTGACTTTTTTTTCTTTTAGACATAATT14220.31887483377920495No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG13670.30654141897058595No Hit
CCTCCCTGATGTACCACCAACTTTACGTTTGCATGAAGGTGGTGAAGAAC13550.3038504921032509No Hit
TTTTCCTTTAGCAAACTTCTGAATGACAACATTTTTCATATGTCTTTATT13310.29846863836858073No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG12870.2886019065216855No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATCAC12710.2850140040319054No Hit
TGATACAGTAGCCAGAATGACGAACACTGACGACATGGTTCTACATGAAA12610.28277156497579287No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC12580.2820988332589591No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC12530.2809776137309028No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATCACGAC12200.2735775648457314No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGAAGGTAATTAAATGTG11660.2614683939427236No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT11410.2558622963024422No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG11220.25160166209582835No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA11060.2480137596060483No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATC10770.24151068634332193No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAAAAAAAGTTATGA10450.2343348813637617No Hit
CGGAATGGTGCATAAGTGTGAAACACTGACGACATGGTTCTACACGATGC10450.2343348813637617No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC9860.22110449093269766No Hit
TGGAACTTTTTTGTTCTGATTGCTACACTGACGACATGGTTCACATTTTC9810.2199832714046414No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC9510.21325595423630372No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATCACGA9460.21213473470824748No Hit
TGTGCTGTGACTGCTTGTAGAACCATGTACAGAGTTGAAAGTCAGGGCAA9400.21078927127457994No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC9210.2065286370679661No Hit
CGATGCGCGGGAAGACGAGACCAAGTCTCTGCTACCGTATCACGACGAAA8780.19688614912668212No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA8550.19172853929762323No Hit
GTAACCAGGCATTATGATCTTTGAAAATTTTTATTTGCGTATCTTTAATT8550.19172853929762323No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC8440.18926185633589943No Hit
TTCCTGAGTTTTCATGGACAGCACTTGAGTGTCATTCTTGGGATATTCAA8300.18612244165734187No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC8210.18410424650684057No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT8160.1829830269787843No Hit
TTGCTCTGAGTTGTATTTGTGTTAACTTATTCTAGAGTTAATTTTTAAAA7880.1767041976216691No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA7830.17558297809361287No Hit
GGAATCCTATGGCTTTCCAACCTACGGTAGCAGAAAGTCAGTCCCATGGA7760.17401327075433407No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG7190.16123136813449254No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC7030.15764346564471243No Hit
AAATGTCCCAAAGCTAAATCCATACTTACTACTAAGCAGTGGCTAGCTGA6770.1518131240988198No Hit
TAGAGTAATGACTTTTGTCGGCCATGCCTCAGGGAAAAGCACGAAGGGTT5980.13409785555553064No Hit
GGAAACACTGATGGGCCTGAAACAGGGTTCAGTGCCATTGACACTGATGA5920.1327523921218631No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT5820.13050995306575056No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC5690.12759478229280422No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT5630.1262493188591367No Hit
ATGAGCAAGTTGGGGTGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCT5150.11548561138979645No Hit
GTAGAGGAGCCGTCAAATCCAGGTGTAAAATGCTGCCTTCTTCAATATAA4770.10696434297656876No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT4710.10561887954290122No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATCA4650.1042734161092337No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG4490.1006855136194536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)