FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250099
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC190227.605788107909269No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA94123.7633097293471787No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA94073.761310521033671No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC85703.4266430493524562No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC77233.087977161044227No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC70972.8376762801930435No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACGTA67622.7037293231880177No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACGT62722.5078069084642483No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT61222.447830659059013No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG57792.310684968752374No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC53872.1539470369733587No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACGTAC48611.9436303223923326No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT45251.809283523724605No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACGT43821.7521061659582806No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACGTACTA41711.667739575128249No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG41551.661342108525024No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT39821.5921695008776524No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTACG38071.5221972099048777No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTACGT37981.5185986349405636No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACGTACT37921.5161995849643541No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTACGTAC34681.3866508862490454No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACG32831.3126801786492548No Hit
ATGGTTGATAGAGTGGTAGACCTTGCAGACCAAGTCTCTGCTACCGTACG32691.3070823953714328No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA31751.2694972790774852No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACGTA30951.2375099460613597No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTACG30341.213119604636564No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA27481.0987648891039148No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG27231.0887688475363757No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACGTACTACGTATC26381.0547823062067423No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTACGTAC25311.011999248297674No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC25000.9996041567539254No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACGTACT24860.9940063734761034No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG23020.9204355075390145No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACGTAC22820.9124386742849832No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTACGTACTAC22570.9024426327174438No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTACGTACT22480.8988440577531297No Hit
TGGTGATAGATGACTTGCTGCTACACTGACCCATGTGGAATAGTTCCCAG21700.8676564080624073No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG20740.8292716084430565No Hit
CACATCCTCCTAAACACACAGGAAGAGAGAGGGACAGGGAGACCAAGTCT19920.7964845921015278No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCAAGACCAAGTCTCTGCTACC18830.7529018508670566No Hit
GGGAAAAGAACAGGCTTCACCTAAAAACGTAGACCAAGTCTCTGCTACCG18300.7317102427438734No Hit
AAAGTGAGGCACTCCAGTTTTATTTAAGTATTCCCAATATAAGTAGACCA16610.664137001747308No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC15550.6217537855009416No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTACG14020.5605780111076014No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC13760.5501821278773605No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA13740.5493824445519574No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC13340.5333887780438946No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG12230.48900635348402033No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT10550.42183295415015654No Hit
AACTGAGCGACCTCAGAGACCAAGTCTCTGCTACCGTACGTACTACGTAT10180.4070388126301985No Hit
TTCAGGAAGAAGAACGATTATCCAGAAAGACCAAGTCTCTGCTACCGTAC9700.38784641282052307No Hit
CATTGGTGCTAAAAGTTTCTTGGATCACATTTTAGACCAAGTCTCTGCTA9280.3710530629870571No Hit
AATATGTGCTTCTTACCAGTCAAATGTCCATGGATCATAGACCAAGTCTC8250.3298693717287954No Hit
CACCATTTATCTGAGGACTGGAGACCAAGTCTCTGCTACCGTACGTACTA8210.3282700050779891No Hit
GTAGACAACATAAAGCCTCATATGTTAATTGCTGCAGACCAAGTCTCTGC7780.3110768135818216No Hit
TTAACTACAATTCCCAGAAGAGCCCAATTCGAGACCAAGTCTCTGCTACC7230.28908552213323524No Hit
TTTTTGTCCAGCCAGCAAATGATCAGCCTTGCAAAGACCAAGTCTCTGCT7110.2842874221808164No Hit
AGTAATCGCATGGTGGTGGTAATTTTAAAGCCCTAGACCAAGTCTCTGCT6970.2786896389029944No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA6400.2558986641290049No Hit
TTCAGGTGTATTTTGGAGGCCAGGTGTGGAGACCAAGTCTCTGCTACCGT6180.24710214754957038No Hit
TTCTGATGAGCCAAGTCTCTGTGAGACCAAGTCTCTGCTACCGTACGTAC5970.23870547263283742No Hit
CTACTCTTAATCTTCCTCTCCAGAATAATCACAAGACCAAGTCTCTGCTA5040.20152019800159138No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAGAGACCAAGTCTCTGCTACC4850.19392320641026153No Hit
ATCCTGTTCCAAGTCAAGACCAAGTCTCTGCTACCGTACGTACTACGTAT4150.16593429002115162No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAGAGACCAAGTC4080.16313539838224062No Hit
AACTGATCACCTTGAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCT3900.15593824845361237No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAATCACAAGACCAAGTCTCTGCT3850.15393904014010454No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC3810.15233967348929825No Hit
TCAATCATTCTCTACAGCCCAGACCAAGTCTCTGCTACCGTACGTACTAC3770.15074030683849196No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC3740.14954078185038724No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTCGTGTTGCCAGACCAAGTC3510.14034442360825114No Hit
TGCTGGAAATGTTTTAGATTCTGGGAGACCAAGTCTCTGCTACCGTACGT3480.13914489862014642No Hit
GCTCTTCCCCCCAAAAAATAAATCAATAAAAGTTTTCAGACCAAGTCTCT3070.12275139044938205No Hit
GCTATTCCTGTCATTCTGTTGAAGGTAAGATAGACCAAGTCTCTGCTACC3030.12115202379857576No Hit
GGGTCTGCAACAAAGCAGGTATTGACAGACCAAGTCTCTGCTACCGTACG3020.12075218213587419No Hit
GGGAAGCTCTTCATCCTCACGAGACCAAGTCTCTGCTACCGTACGTACTA2930.11715360717156006No Hit
GCTATTCCTACCATTCTGATGAGGGAGACCAAGTCTCTGCTACCGTACGT2890.11555424052075378No Hit
CTTTTTAGGAGAACCCTCAATCAAAAGAAACTGAGCAAGCCTAGACCAAG2840.11355503220724593No Hit
GCCACTCCCACATCTCCTTACCCTAGACCAAGTCTCTGCTACCGTACGTA2660.10635788227861766No Hit
TGCTAATTGGGCAGATAGAGCTTCGTTCAGACCAAGTCTCTGCTACCGTA2590.10355899063970667No Hit
AGTAATCGCATGGTGGTTGATCTTAAGGTAACATAGACCAAGTCTCTGCT2550.1019596239889004No Hit
GTAACTTGATTTGCTGTTGTAGAATGCACTGACTACAGACCAAGTCTCTG2550.1019596239889004No Hit
CGATGCGCGGGAAGACGAGACCAAGTCTCTGCTACCGTACGTACTACGTA2540.10155978232619883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)