FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250099
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG189307.569002674940724No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT94113.762909887684477No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT93293.730122871342948No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG85533.4198457410865295No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG76413.055190144702698No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA70512.819283563708771No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT67612.703329481525316No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA62692.5066073834761435No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT60812.4314371508882484No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC57252.289093518966489No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA54032.160344503576584No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC48591.9428306390669294No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT45051.8012866904705735No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA43751.7493072743193696No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC41751.6693389417790554No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT40941.636951767100228No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG39371.5741766260560817No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA38131.5245962598810872No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG38011.5197981599286683No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT37961.5177989516151604No Hit
CACGCACACACAAGCAGATTGCTTGTAGAACCATGTCGTCAGTGTAGATC34811.3918488278641659No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG32771.3102811286730454No Hit
GCAAGGTCTACCACTCTATCAACCATTGTAGAACCATGTCGTCAGTGTAG32411.295886828815789No Hit
CCAACTGGCCAAGACCTCTTCTTTTATATCTGATGTAGAACCATGTCGTC31611.2638994957996634No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT30721.2283135878192237No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG30211.2079216630214435No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT27281.0907680558498836No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT27071.0823713809331506No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT26401.0555819895321454No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC25121.0044022567063442No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA25021.0004038400793285No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT24800.991607323499894No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC22750.9096397826460721No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC22510.9000435827412344No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG22500.8996437410785328No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT22220.8884481745228888No Hit
GGGAACTATTCCACATGGGTCAGTGTAGCAGCAAGTCATCTATCACCATG21500.8596595748083758No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC20810.8320705000819675No Hit
CCCTGTCCCTCTCTCTTCCTGTGTGTTTAGGAGGATGTGTGTAGAACCAT19820.7924861754745121No Hit
TGAGATTGCCTTGAAGTTGTGCATCAAACTCTGTAGAACCATGTCGTCAG18850.7537015341924598No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCTGTAGAACCATGTCGTCAGT18090.7233135678271404No Hit
ACTTATATTGGGAATACTTAAATAAAACTGGAGTGCCTCACTTTTGTAGA16430.6569398518186798No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG15270.6105582189452977No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA13800.5517814945281668No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG13780.5509818112027637No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA13330.532988936381193No Hit
TTGTGAAGAGAACATGATTGGCAATACTCTGAAGTATCTACACAATAAAA13140.5253919447898633No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT12280.4910055617975282No Hit
GCATCTTTTACATTGGATATTACTTTGGATGTAGAACCATGTCGTCAGTG10590.4234323208009628No Hit
CTGAGGTCGCTCAGTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG10320.4126365959080204No Hit
TTCTGGATAATCGTTCTTCTTCCTGAATGTAGAACCATGTCGTCAGTGTA9620.38464767951891055No Hit
AAAATGTGATCCAAGAAACTTTTAGCACCAATGTGTAGAACCATGTCGTC9160.3662549630346383No Hit
CCAGTCCTCAGATAAATGGTGTGTAGAACCATGTCGTCAGTGTAGATCTC8220.32866984674069066No Hit
ATGATCCATGGACATTTGACTGGTAAGAAGCACATATTTGTAGAACCATG8100.32387174678827185No Hit
GCAGCAATTAACATATGAGGCTTTATGTTGTCTACTGTAGAACCATGTCG7770.31067697191912No Hit
CGAATTGGGCTCTTCTGGGAATTGTAGTTAATGTAGAACCATGTCGTCAG7270.29068488878404153No Hit
AGGGCTTTAAAATTACCACCACCATGCGATTACTTGTAGAACCATGTCGT7250.2898852054586384No Hit
TTGCAAGGCTGATCATTTGCTGGCTGGACAAAAATGTAGAACCATGTCGT7000.27988916389109914No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT6420.25669834745440806No Hit
CCACACCTGGCCTCCAAAATACACCTGAATGTAGAACCATGTCGTCAGTG6190.24750198921227193No Hit
CACAGAGACTTGGCTCATCAGAATGTAGAACCATGTCGTCAGTGTAGATC5940.2375059476447327No Hit
TGTGATTATTCTGGAGAGGAAGATTAAGAGTAGTGTAGAACCATGTCGTC4960.1983214646999788No Hit
CTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG4770.19072447310864898No Hit
TGACTTGGAACAGGATTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4250.16993270664816734No Hit
CTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA4070.16273555671953907No Hit
CACGCACACACAAGCAGATTCAAGGTGATCAGTTTGTAGAACCATGTCGT3960.1583372984298218No Hit
TGTGATTATTCTTAGGTAGGAAGCTAAAGAGTAGTGTAGAACCATGTCGT3790.1515399901638951No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG3750.14994062351308882No Hit
GGGCTGTAGAGAATGATTGATGTAGAACCATGTCGTCAGTGTAGATCTCG3730.14914094018768567No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG3690.14754157353687938No Hit
CCCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA3490.139544740282848No Hit
GGCAACACGAAAGGTAAAATTGAGAGGTCAGAAGAGAAATTGTAGAACCA3480.13914489862014642No Hit
GTCAATACCTGCTTTGTTGCAGACCCTGTAGAACCATGTCGTCAGTGTAG3080.12315123211208362No Hit
ATCTTACCTTCAACAGAATGACAGGAATAGCTGTAGAACCATGTCGTCAG3020.12075218213587419No Hit
CCCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA2860.11435471553264907No Hit
CGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC2830.11315519054454436No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAGAGCTGTAGAACCATGT2830.11315519054454436No Hit
AGGCTTGCTCAGTTTCTTTTGATTGAGGGTTCTCCTAAAAAGTGTAGAAC2800.11195566555643964No Hit
AGGGTAAGGAGATGTGGGAGTGGCTGTAGAACCATGTCGTCAGTGTAGAT2660.10635788227861766No Hit
GAACGAAGCTCTATCTGCCCAATTAGCATGTAGAACCATGTCGTCAGTGT2660.10635788227861766No Hit
CGTCTTCCCGCGCATCGTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG2560.10235946565160195No Hit
GTAGTCAGTGCATTCTACAACAGCAAATCAAGTTACTGTAGAACCATGTC2550.1019596239889004No Hit
ATGTTACCTTAAGATCAACCACCATGCGATTACTTGTAGAACCATGTCGT2530.10115994066349726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)