FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379254
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA206205.4369894582522535No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT193735.108186070549025No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA176914.664683826670252No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC165034.351437295321869No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC115703.050725898738049No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG95572.5199470539532873No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT82902.1858701556212985No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT78132.0600969271253566No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG76132.00736182083775No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC71791.8929266401936433No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG71591.8876531295648826No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG70621.8620766030153935No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA66331.7489598000284772No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA64081.6896328054549195No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC64041.6885781033291674No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA60811.6034109066746824No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG51611.3608294177516915No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTACCGTAGTGCTAAAATGGA49211.2975472902065635No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG46861.2355835403186255No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT46821.2345288381928734No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG44911.184166811688209No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA43911.1577992585444057No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT42301.1153474979828821No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC35470.9352571100107052No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC35420.9339387323535151No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG31810.8387518655043849No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG31000.8173941474579042No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA29980.7904992432512248No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC27850.7343363550549236No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC27720.7309085731462291No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA27020.7124512859455668No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT26710.7042773444709878No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA26620.7019042646880456No Hit
TGTGGTCTGCCAGCTAAAGGAGATTCTCTGTTAGACCAAGTGGTTTGTAC26020.6860837328017635No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG25920.6834469774873831No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG24370.6425772701144878No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT24300.6407315413944217No Hit
TCCCTGCCAGCGAGATCTCCTCCATCTTGGAGAAAGGAGAACGCCTCCCT23400.6170007435649987No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA23180.6111998818733619No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG20960.5526639138941185No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG20280.5347339777563321No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC19560.5157493394927938No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC19530.5149583128984796No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT18680.49254589272624677No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAAGACCA18270.48173519593728736No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG17620.46459628639381517No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG16290.4295274407125568No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTCGTCGTCTAT15990.4216171747694158No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC15340.4044782652259436No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC15120.3986774035343068No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGCTACCGATGCCGACGAGTACCTCA13620.3591260738186018No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG13430.3541162387212791No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCAGGGACGAACTGGTGTAATG12240.32273885048015316No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT11360.2995354037136062No Hit
GTAGAGGAGCCGTCAAATCCAAGTCTCTGCTACCGTATCAAGAGGGATAA10980.28951573351896087No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA10590.27923238779287757No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA10120.26683963781529No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA9800.25840202080927294No Hit
CAGCCTCTGGCATTCTGGAAGGGTGAGAGCAGTCAGAGGACCAGGAGACC9780.2578746697463969No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAACCACAGGCTCCCAG9270.24442721764305714No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC8790.2317707921340315No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT8610.22702463256814692No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT8480.2235968506594525No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGAAT8210.2164776113106256No Hit
GGAATCCTATGGCTTTCCAACCCTGACGACATGGTTCTACAGCAACAGAG7770.20487588792735212No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC7540.19881135070427733No Hit
GGATGCAGAGCTTCTTCCCATGCTTGCTAAGACCTGGGTGCGGAAGAGAA7490.19749297304708716No Hit
GGGGTCAGAGGCAAGCAGTCACAGCACAGGAGATAACACAGGCCCAAGAG7260.1914284358240124No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGGGAGGTGGCTTTAGGTCAGC7050.1858912496638137No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC7000.1845728720066235No Hit
GGAAACCGTAGCTGCCCTGGGTACACTGACGACATGGTTCATCTGGACCT6520.1719164464975979No Hit
TCTTAAATGGCTACGACCCAGCACCTGACCCGCTACCTGTTAAAGAATCA6370.16796131352602742No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC6350.16743396246315134No Hit
ACTTCTACCGTGCCCTGAAGCTGTGTCTACTCATTTGAACAGACCAAGTC6350.16743396246315134No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC6230.16426985608589495No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC5640.14871299973105095No Hit
GGAATCCTATGGCTTTCCAACCGTCAGCAACAGAGGAGGGGGAGACCAAG5430.14317581357085224No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCTCAG5330.14053905825647192No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5210.1373749518792155No Hit
GGTCTTCTCTGTTTCAGGGCATGCTGATGCAGGAGGTGGCTTTAGGTCAG5140.13552922315914928No Hit
TCCCTGCCAGCGAGATGTACACTGAGGCCTTTGCATCCCTAGACCAAGTC5050.13315614337620696No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC4950.13051938806182664No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTC4500.11865398914711514No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCCA4230.11153474979828822No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCGAGTC4200.11074372320397412No Hit
GGATGCAGAGCTTCTTCCCACAGGTCTCTAAGGACTTGCTTTATGAATCT4170.10995269660966003No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT4040.10652491470096559No Hit
CCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA4020.10599756363808951No Hit
TGTGCTGTGACTGCTTGTAGTACGGTAACAGAGTTGAAAGTCAGGGCAAG3900.10283345726083311No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATAACA3890.10256978172939507No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT3860.10177875513508097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)