FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379254
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC184844.873778523100613No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA167914.427375848376022No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA161454.257041455067053No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA138653.6558612433883355No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG110722.919415484081908No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG98352.5932488516930605No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC84912.2388689374403437No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC78902.080399943046085No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC77642.0471768260848933No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG76322.0123716559350724No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA68081.7951030180301328No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA66211.7457956936512204No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG65461.726020028793368No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA64241.693851613957928No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA62401.6453353161733297No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC54191.4288577048627042No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT50991.3444815348025334No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTACCAGAGACTTTGTCATATT49421.3030844763667622No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT45641.2034151254831853No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT45501.199723668043053No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCT44101.1628090936417284No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA43661.1512073702584547No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA43601.1496253170698265No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC41361.090561998027707No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC40051.0560205034093246No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA37390.9858828120468076No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA34300.9044070728324552No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA34250.903088695175265No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT33000.870129253745511No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT29720.783643679433836No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG29650.7817979507137698No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC27960.737236785900742No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC27800.7330179773977334No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT27570.7269534401746587No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA27230.7179884721057656No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT26460.697685456185037No Hit
TCTCCCAATGAAAGTAAAGTACAAACCACTTGGTCTAACAGAGAATCTCC25590.674745684949928No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT25430.6705268764469194No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT24550.6473234296803725No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT23290.6141003127191803No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT22490.5930062702041377No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG19860.5236596054359347No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT17040.4493031055704093No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT17000.44824840344465716No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG16210.4274180364610525No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG16000.42188085030085376No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGT14000.369145744013247No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG13390.35306153659552697No Hit
ATGAGGTACTCGTCGGCATCGGTAGCAGAGACTTGGCTATAGGAGCAGGG13000.3427781908694437No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGATTCATT12490.32933073876610397No Hit
TCATTACACCAGTTCGTCCCTGGTGGTAAAAAGATAACCTCAGAATAAGA11820.31166447815975573No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT11220.2958439462734737No Hit
TTCATGGTGTTTTATCCCTCTTGATACGGTAGCAGAGACTTGGATTTGAC10770.2839785473587622No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC10520.2773866590728114No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT9950.26235715378084346No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT9820.25892937187214904No Hit
CCTGGTCCTCTGACTGCTCTCACCCTTCCAGAATGCCAGAGGCTGTGTAG9810.25866569634071096No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT8750.23071609000827942No Hit
GTTCAAATGAGTAGACACAGCTTGGTCTTCTCTGTTTCAGGGCACGGTAG8510.2243878772537666No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA8430.22227847300226233No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAAGCTCA8230.21700496237350167No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGGGTTGGAAAGCCATA7760.20461221239591407No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG7590.2001297283614675No Hit
TTCTCTTCCGCACCCAGGTCTTAGCAAGCATGGGAAGAAGCTCTGCATCC7390.19485621773270684No Hit
CTTGGGCCTGTGTTATCTCCTGTGCTGTGACTGCTTGCCTCTGACCCCTG7220.19037373369826027No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGAC7200.1898463826353842No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA6950.18325449434943336No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC6930.1827271432865573No Hit
GCTGACCTAAAGCCACCTCCCTTCTACCATGCCCTGAAACAGAGAAGACC6890.18167244116080514No Hit
AAGACCCAGGTCCAGATGAACCATGTCGTCAGTGTACCCAGGGCAGCTAC6400.16875234012034152No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG6290.16585190927452315No Hit
GTTCAAATGAGTAGACACAGCTTCAGGGCACGGTAGAAGTTGTAGAACCA6260.16506088268020905No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA6140.16189677630295263No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC6080.16031472311432443No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG6010.1584689943942582No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA5940.15662326567419196No Hit
TCCAGATGATTCTTTAACAGGTAGCGGGTCAGGTGCTGGGTCGTAGCCAT5930.1563595901427539No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC5920.1560959146113159No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGGGCAGCTACGGTTT5620.14818564866817488No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGATAGTAAGATACAGTCTA5590.14739462207386078No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCC5450.14370316463372831No Hit
CCCCCTCCTCTGTTGCTGACGGTTGGAAAGCCATAGGATTCCTGTAGAAC5390.14212111144510012No Hit
TCATTACACCAGTTCGTCCCTTTCCAACTTTACAGTGAATTGCTGCAACA5290.13948435613071977No Hit
GCTGACCTAAAGCCACCTCCTGCATCAGCATGCCCTGAAACAGAGAAGAC5080.13394716997052108No Hit
AGGGATGCAAAGGCCTCAGTGTACATCTCGCTGGCAGGGATGTAGAACCA5040.13289246784476896No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT5030.1326287923133309No Hit
ATCTCCGCAAGAAAGGGGAGTTATGCCTCAGATTCACTTTTATCACCTTT4770.12577322849594202No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA4500.11865398914711514No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG4110.10837064342103181No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT3980.10494286151233737No Hit
TTCTCTTCCGCACCCAGATTCATAAAGCAAGTCCTTAGAGACCTGTGGGA3970.10467918598089934No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTGCCACCATCCAC3930.10362448385514722No Hit
TGCCCTGACTTTCAACTCTGTTACCGTACTACAAGCAGTCACAGCACATG3890.10256978172939507No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGAGC3840.10125140407220491No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG3800.10019670194645278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)