FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751364
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA255123.3954248539988607No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG244493.253948818415575No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC228273.0380747547127624No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA203522.7086738252032303No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG177492.3622372112584578No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT172442.2950261125100484No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC157862.100979019489888No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA156402.0815476919309415No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT152672.031904642756374No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG149511.9898477968068737No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC147491.9629633573075103No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT143461.9093275695934326No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA142511.896683897551653No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA141641.8851049557870752No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT140941.8757885658615532No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT131331.7478878413125996No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT131121.745092924334943No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC130781.7405678206568322No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA128591.7114208293184128No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC125531.6706948962154162No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG124211.6531268466415745No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT123381.6420802700155983No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG121011.6105376355534735No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA119851.595099046534037No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT117641.565685872626317No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG108641.4459037164410327No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC108591.4452382600177809No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT99061.31840226574603No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG94491.2575795486608354No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT85711.140725400737858No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG84101.1192977039091572No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG83661.1134416873845432No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG80781.0751113974052522No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG75271.0017780995629282No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG74730.9945911701918112No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG71310.9490739508414031No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC70980.9446819384479426No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC70660.9404230173391325No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA70120.9332360879680155No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC64540.8589711511331392No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT57930.7709978119792803No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC47010.6256621291411353No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC41050.5463397234895471No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTGGCTTCGTAT33710.44865072055621513No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA31020.41284916498528007No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT30020.3995400365202485No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA30010.39940694523559817No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA17530.23330902199200387No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA16210.21574097241816217No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA16000.21294605544050554No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG15970.2125467815865546No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA14380.19138526732715436No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC12300.16370228011988863No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG11610.15451898147901683No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT11320.15065933422415767No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC9900.1317603718038128No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG9860.13122800666521153No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG9010.11991524746993469No Hit
GACCCAAAACCCAAAATGGCAGGAGAGGGAGAGATGGGGGTGGGAGGCTG9000.11978215618528437No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGAGAGGCTG8020.1067392102895534No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG7820.10407738459654708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)