FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364600
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAACCAGGCATTATGATCTTTGAAAATTTTTATTTGCGTATCTTTAATT67441.8496982995063083No Hit
AAAGCAGAAGCCTTAGAAACTCTGCAAATTATCAGAAGAGAATGTCTCAC64471.7682391662095447No Hit
TTGCACAAGGTTTGAATCAAGTAAATTTGAAATAGATTGAAGTTAAATAC62911.7254525507405374No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG58951.6168403730115195No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT53881.4777838727372463No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC52941.4520021941854087No Hit
GGATTTGGAAAAACATCAGGGAATTCATTTAAAGTAAATAGCTGCAAAGA51961.4251234229292375No Hit
AGGTTGACTTAGAATCTCACTTTCCTGAAGATTTTCATTCCTGCCATCAA51471.4116840373011519No Hit
GGAAACACTGATGGGCCTGAAACAGGGTTCAGTGCCATTGACACTGATGA50781.3927591881513988No Hit
CTACTACTATATGTGCATTGAGAGTTTTTATACTAGTGATTTTAAACTAT50081.3735600658255622No Hit
AGGATATTTTTGATTGCTCTAGGGATTTATTTTCTGTTACCTTTGATTTA50051.3727372462973122No Hit
GGTAGGGCCACCTGCATTTAGGATAGCCAGTGGTAAAATCGTTTGTGTTT48921.3417443773998903No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG48081.3187054306088863No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC46991.288809654415798No Hit
GCTACTGTGTGAACCTCATCAACCATCTCATGATTATCTTTAATAGAGTC46731.2816785518376304No Hit
CCGAGATTCAGTTTAGGAGTTAATGTTTTATTTCAATGAAAGTAAAATAA46711.2811300054854635No Hit
TCTGAACTCCAAGATCAAATCACCCGTGATGCTAATAGTTTTAAATCTCG46661.2797586396050467No Hit
AAGCCACCACCTAGAATCGAAAGGGGCTTGAAGTTAATGTCAAAGGTGAA46541.276467361492046No Hit
AGATAACTCTGTCATTTTCCTACTTGTTCAGTCCATGGTGGTTGATCTTA45501.2479429511793747No Hit
GATACCTAACTTGTCATTATCTGGAGTTCAAAAACACTACCTTAGATCAT45331.243280307185957No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT44541.2216127262753702No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC44231.2131102578167856No Hit
AGACTTCAAGGTTTGTAACGGGAATGCCAGAAGAGGCATCAAAGTCCCAA43331.1884256719692814No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA43251.1862314865606143No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGCATTCAGATCTACCTTTTTTT43061.18102029621503No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA42521.1662095447065277No Hit
TGGATCCTTTATTACGAATTGTGATCACATCCTCTGATTGGCAACATGTT41481.137685134393856No Hit
GTTGAACTTCGAAATATGTTTGGTGAAACACTTCATAAAGCAGTGCAAGG41121.1278113000548546No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA38781.063631376851344No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC38691.0611629182665936No Hit
TTCAGAAGACAATGTTGATGTTCATGATATAGAATTGTTACAGTATATCA38691.0611629182665936No Hit
AGAGTCAAGAACTGTTTTTAAATTGTTTGTACTATAACACCTTAATTTGA38591.0584201865057599No Hit
AGTGAAGAAGAAGTTTGTGTTGATTTTAACTTAATAACTGATGATTGCTT38231.0485463521667582No Hit
GAGTCACAGTCACAGGTAGGTTGTCCTTGCCTGCCTGACACTTGCAGGGT38211.0479978058145913No Hit
AAAGTGAGGCACTCCAGTTTTATTTAAGTATTCCCAATATAAGTTATATA36190.9925946242457487No Hit
TTACTGCAGCTCACAAATGCTTTTTTACATCTGCAAGAAATTAACTAGTC34350.9421283598464071No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC33730.9251234229292375No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT32650.8955019199122326No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT32390.8883708173340648No Hit
TTTAGGGTTTTTCATTCTTTTTTGGTCCAAACTTTTCATTTCTGCTTTTA30720.8425671969281404No Hit
CAGTAAATCATAGTCTTTCCTTCATAAGTGACGGCAATGTGCTTGCTTTA30550.8379045529347229No Hit
CAGCAATGTGGCTTTAAGTAGAATTGACTGTTTTCGTTTGTGCTATTTTT29910.8203510696653867No Hit
GTAGACAACATAAAGCCTCATAATTACTCTGTTATTTTTCTTTTAGGCAT28690.7868897421832145No Hit
CACCATTTATCTGAGGACTGGAGATAACAGATGGTTGGCTACAGAAGGAT28660.7860669226549645No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC28160.7723532638507954No Hit
ATATATGTCTTCCACCCTTGACTCTCAGGATAGTGCAGCAGGATGCAGCG27410.751782775644542No Hit
CCAGCTGGATAGTAAATGTAACACCAAGTTCTGACGAAAAGGATTTCATT26700.732309380142622No Hit
ATGAGCAAGTTGGGGTGTGCAGCAAGTTCGTCCAGCAACTTCTGTAGATG25750.7062534284147011No Hit
TGGGGAAAAATATGCTTTGATCTACAGAAAAACATAGAAATCAGAGACCA24900.6829402084476138No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC24780.6796489303346133No Hit
AACAATCGACAGGCTAGAAGTTGCAGAATTTATCATCTTGCAACTTCAAA24520.6725178277564454No Hit
GTCACTGGTTAAAACTAAGGTGGGATTTTTTTTTTAAATAGATTTAGGAC23360.6407021393307735No Hit
TGTCATGATTCTGTTGTTTCAATGTTTAAGATAGAAAATCATAATGATAA22380.6138233680746022No Hit
TGCAGGGCTGATTGTCTTCTTTTAAGGTAAAATGTATGGAGCAGGCATAA21480.5891387822270981No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA21170.5806363137685134No Hit
TGGGGAAAAATATGCTTTGAGCTACAGAAAAACATAGAAATCAGAGACCA20590.5647284695556775No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA20520.5628085573230938No Hit
GAGAAAGCCCCCCTACTGCTACCGTATCCAGTTTTATTTAAGTATTCCCA20410.5597915523861766No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC19810.5433351618211739No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC19600.5375754251234229No Hit
TCAAAGGGCTCCACTGGTTTTTCTTATCTCTTTCATTTGATTAGACCAAG17420.4777838727372463No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC17050.46763576522216127No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC17030.4670872188699945No Hit
ACCAGTTTGCTCAATGGTGTAGATTGGCGCTGGAATCCGAATTAGACCAA16910.46379594075699393No Hit
AAAAGCAGTGGTCCAATGTATCTGCCATCTATTGTGATGGCCACGTTAGA16180.44377399890290725No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA15830.4341744377399891No Hit
CTGGTTCAGAGGAAGTATCATGTCTGTGAAAGGTTAATTATCCTAGTAAT15670.429786066922655No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACAG14500.39769610532089966No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT14230.39029072956664834No Hit
GCTTCCAGTACTTCAACTTTGCCACACTGACACAATGGCAACAGTTCTGA14070.38590235874931433No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGA14010.38425671969281405No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG13910.38151398793198027No Hit
ATACGGCCAGGCATTGAAGTACATGACGACAGGGGCCAGGAGGAGACCAA13240.3631376851343938No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG13160.36094349972572687No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT13090.35902358749314317No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC13040.35765222161272625No Hit
GCTGCCCCAAAGTGTAAAGAAGCCAAAATGATAAGAAACTAAGAGACCAA13030.3573779484366429No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACAGG13030.3573779484366429No Hit
GCTGCCCCAAAGTGTATAGAAGCCAAAATGATAAGAAACTAAGAGACCAA12730.34914975315414154No Hit
TTGGACTCAATTTCAACTCTAACTTTAAATGCAAACTAGGCCAGGCAGAC12480.34229292375205705No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG12240.33571036752605593No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACAGGTCA12110.33214481623697206No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC10930.2997805814591333No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT10910.2992320351069665No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCAAA10710.29374657158529893No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA10670.29264947888096543No Hit
TCAAAGGGCTCCACAGTTTTTCTTATCTCTTTCATTTGATTAGACCAAGT10380.28469555677454744No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC9830.2696105320899616No Hit
TCTCCCCCACCCCCTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT9490.2602852441031267No Hit
GTGGACAAGTTTGACATTATTGGGATCTTGGTCTGCAATGGTGAAGAACA9400.2578167855183763No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACA9210.2526055951727921No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA9190.25205704882062535No Hit
GTCACTGGTTAAAACTAAGGTGGGATTTTTTTTTAAATAGATTTAGGACC9090.24931431705979154No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACAG8890.24382885353812395No Hit
TCTCCACTTCACCCCGTCAGTGTAATAAACTGCTGTTCTCATGCTGAGAC8720.23916620954470652No Hit
GTCTCGGAGGCTGGCATCTTCTGCTTCTGTTTCAAAAGACCAAGTCTCTG8390.230115194733955No Hit
AAAAGCAGTGGTCCAATGTATCTGCCGTCTATTGTGATGGCCACGTTAGA8290.22737246297312125No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA8230.22572682391662097No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT7900.21667580910586945No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG7870.2158529895776193No Hit
TCCAACATATGCATTGGAAAAGATAACTTTCATATACATTAGTGTAATTG7680.2106417992320351No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACAGGTC7210.1977509599561163No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACAGGT7180.19692814042786616No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC7110.1950082281952825No Hit
GGGGTCAGAGGCAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGT6810.18678003291278114No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC6310.1730663741086122No Hit
TCTCCCCCACCCCTTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT6030.16538672517827757No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACAGG6020.16511245200219418No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACAGGTCACATATC5990.16428963247394404No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA5750.15770707624794295No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTAAAATGAAATACTTTGAGAA5690.1560614371914427No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG5600.15359297860669227No Hit
CTTTCTTCAGAAGCTCCACCCTACACTGACGACATGGTTCTACACGTTCT5400.14810751508502468No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT5350.14673614920460779No Hit
GGGTCTGCAACAAAGGCATACACTGACGACAGGGTTCAGTGCCATTGACA5330.14618760285244103No Hit
GTGCAGGCTGATTTTCTTTTTCCACACTGACGACATGGTTCTACAGTGCA5170.14179923203510697No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA5050.13850795392210644No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA4950.13576522216127263No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC4890.13411958310477234No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTACA4810.1319253976961053No Hit
GAGAAAGCCCCCCTACTGCTACCGTAACATCTGCAAGAAATTAACTAGTC4760.13055403181568842No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCCCACAGCAGGGTCTTC4500.12342292923752057No Hit
GTTGTTTCAGAACACCGAAAGCTCAAGAAGCATGTCATAGACCAAGTCTC4350.11930883159626988No Hit
GTAACTTGATTTGCTGTTGTATTTGCTACCGTAACCCTCAATTTGGAAGC4160.11409764125068567No Hit
TCTGGGGTATCAGGTAGGTGTCACACTGACGACATGGCTCTAATCAATCG4110.11272627537026879No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA4080.11190345584201865No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACAGGT4030.11053208996160177No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG3970.10888645090510148No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC3740.10257816785518377No Hit
GTCACTGGTTAAAACTAAGGTGGGATTTTTTTTTTTAAATAGATTTAGGA3730.1023038946791004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)