FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364600
Sequences flagged as poor quality0
Sequence length150
%GC38

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCCACCCCCAAGAATATCATCTAGTGCTGAACAGAAGGTGATTATGA63121.7312122874382885No Hit
AGTGACGTGTCCTCTGTTCCTTCTCTTTCTTCACAGGTTTTGAAGGAAGA62971.7270981897970379No Hit
TGTTCATCATTGGACTACGCAAAGATTTCATGTAAATAGCTCTCATTTCA58881.6149204607789358No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT57171.5680197476686781No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT52911.4511793746571586No Hit
GCCAACTGCCCACAAGTTCTAATTTAGAGGCAAATATTTCATGTTCTCTA50131.374931431705979No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG49301.3521667580910586No Hit
TCCCCTGGAAACTGCACTGACCCTGTGCCCTGGTAACTGCTGGACTCCCC49271.3513439385628085No Hit
GAAGAATATCCTCTGAATCATCCAATGCCTCGTAACAACCTGCCATAATT47651.3069116840373012No Hit
TCTCTTGGTATTAGTGCTTGATTCGAAACATAATTTGCATCACTTATCTC47021.2896324739440483No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG46891.2860669226549644No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC46851.284969829950631No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC46331.270707624794295No Hit
ACAGAGGACTTACCATGACTTGCAGCTTCTCTTTGATTTGTGTTTTCACT45881.258365331870543No Hit
AAAGCACATAACTGAAAACCATAGGGTATTTCAAATATAAATGTAAGAGG45551.2493143170597916No Hit
TGGCTGTAATTTGGTTAGTGCTATTACTGTAGCTTCTATCAGAACTTGAC45011.234503565551289No Hit
GCAAGGTCTACCACTCTATCAACCATAAAACTTCCCTCTGTATCAATAAA44791.2284695556774547No Hit
GTGTCCAACCAATGCAAAGTTAAAGTTAGAGTTGAAATTGAGTCCAACAA44541.2216127262753702No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC44491.2202413603949533No Hit
ACAACAAAAGCAAGTCCTATGAACTTATCAACGAAGAGTCAGAACTTTAA43861.2029621503017005No Hit
TGCTATCTGATGTATGTTCCAATATTTCATCATCAGAATCTGGACTACAG43491.1928140427866154No Hit
CAGCATGAGAACAGCAGTTTATTACTCACTAAAGACAGAATGAATGTAGA42701.1711464618760286No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT42041.1530444322545255No Hit
TTCTATAAAGAGAATGTATAGAGAAATCTACATTGATGTAAAACCAATTA41711.143993417443774No Hit
TCCTAGTCTTGCTAGTTCTTACTTTTTGTAGATTTTTTGTTCTACATTTA41591.1407021393307735No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC38941.068019747668678No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG38631.0595172792100933No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG38551.0573230938014262No Hit
CTCTGACTCCACCTTTCCACTTGCCCAGTATTGTTGGTGTTTTTCTTCTT36380.997805814591333No Hit
CCTCAAGGCTCCTATGAAAAAGCATCTACAGAAGTTGCTGGACGAACTTG36370.9975315414152496No Hit
TCTTTTATCATTTACATTGTTCTCCTCCTCACTTGAATCATCTGGTAAAA36360.9972572682391662No Hit
ATAACCAAAGCAGCAGGAATAGGATAAAGACACTAAAAAAAATGCAATAG36000.9873834339001646No Hit
TGGAGTTGAAGCCAGCTGATTATAAGATGGTTTCCTTTGTGGAGTTTTAA34790.9541963795940757No Hit
AAACTTCTATGGAATGCTTTTACGAATCTTTTAAAAGTAATCATTTAAAA34410.9437739989029073No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA33450.9174437739989029No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT32390.8883708173340648No Hit
TCATGATACTAGTTTTTGACCCAGTGATTTTTTTCAGATATATTATACAT32370.8878222709818979No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC32100.8804168952276468No Hit
GGACCACTTGGGATCATTTGCATGCAGTATATGCATAAGCTTGTTTTCTG30580.8387273724629731No Hit
AAGCACTATTCATGACCAATAATTGTATTAATCTAAATTTAAACGGAGAG30480.8359846407021393No Hit
CAAAATCTAAAGCAGCATACCTTTGTAGAACTTCATCACAGGTGAGTTCC29910.8203510696653867No Hit
CTCACCATTACCATTCCAAATATTCTTCCATTTTTTAAATATGTCATTTT29790.8170597915523862No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG27800.7624794295117938No Hit
TTCTTACCTCCGTGCAACATAATAAAAAATAGGATTCCCAGCTTTGGAAG26800.7350521119034559No Hit
TTTGCCCAGCCTCTTCTAAAGATAATTTTATTTATCTAATGTTGTTTTCT26110.7161272627537026No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTTTAAATACGGTTGCG25690.7046077893582008No Hit
TAATGATCAAGGGGAGAAGGTGCCTTGCTTCATGCAGTGTTAGTAAAACA25630.7029621503017005No Hit
AGGGCTAAAAAGGCTTCTGCAAGATCTGGAAAAAAGCAAAAAAAGAAAAC25280.6933625891387822No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA24500.6719692814042787No Hit
CTGATTTCTATGTTTTTCTGTAGATCAAAGCATATTTTTCCCCATGTAGA24450.6705979155238618No Hit
TTTGAAGTTGCAAGATGATAAATTCTGCAACTTCTAGCCTGTCGATTGTT23870.6546900713110257No Hit
ACTGGCAGCAGTATATTTGTTATCTTCATTTTCAGTATTTCTCTTGTAAT22970.6300054854635218No Hit
GCTAACATGTTGCCAATCAGAGGATGTGATCTCAATTCGTAATAAAGCAT21260.5831047723532639No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT21180.5809105869445969No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT20750.5691168403730116No Hit
CTGATTTCTATGTTTTTCTGTAGCTCAAAGCATATTTTTCCCCATGTAGA20340.5578716401535929No Hit
ACACCGGATATCTGCTTCCTCACAGAGGTGGCGGAAACAGGACATGGCAA19910.5460778935820078No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA19680.53976961053209No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG19600.5375754251234229No Hit
ACTTATATTGGGAATACTTAAATAAAACTGGATACGGTAGCAGTAGGGGG19340.5304443225452551No Hit
AATCAAATGAAAGAGATAAGAAAAACCAGTGGAGCCCTTTGATGTAGAAC17300.47449259462424576No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG17010.4665386725178277No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG16910.46379594075699393No Hit
AATTCGGATTCCAGCGCCAATCTACACCATTGAGCAAACTGGTTGTAGAA16840.4618760285244103No Hit
AACGTGGCCATCACAATAGATGGCAGATACATTGGACCACTGCTTTTTGT16040.43993417443774No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT15680.43006034009873834No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA14500.39769610532089966No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC13940.3823368074602304No Hit
CAGAACTGTTGCCATTGTGTCAGTGTGGCAAAGTTGAAGTACTGGAAGCT13920.38178826110806363No Hit
GGAGAGCTGTAAATTCTGGCTTGTAGAACCATGTCGTCAGTGTAGGGTGG13640.374108612177729No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCAGAGACTTGGTCTGTCTAAGAA13620.3735600658255623No Hit
CCTCCTGGCCCCTGTCGTCATGTACTTCAATGCCTGGCCGTATTGTAGAA13220.3625891387822271No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC13190.36176631925397695No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT13050.35792649478880967No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT13000.35655512890839275No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG13000.35655512890839275No Hit
CTTAGTTTCTTATCATTTTGGCTTCTTTACACTTTGGGGCAGCTGTAGAA12810.35134393856280854No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACACTTTGGGGCAGCTGTAGAA12570.3447613823368075No Hit
TAGAGGAGAGACTTTGCTCCATATTGCTTCTATTAAGGTAGGATGCTTAC12410.3403730115194734No Hit
GCCTGGCCTAGTTTGCATTTAAAGTTAGAGTTGAAATTGAGTCCAATGTA12390.33982446516730663No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT12240.33571036752605593No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC12180.33406472846955565No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG10970.3008776741634668No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT10800.29621503017004935No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT10680.2929237520570488No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGAGACTTGGTCTGTCTAAGAACA10670.29264947888096543No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT10300.28250137136588044No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGGAGCCCTTTGATGTAGAACC10200.2797586396050466No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT9840.269884805266045No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG9830.2696105320899616No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG9240.25342841470104227No Hit
TGCCTGAATGTTCTTCACCATTGCAGACCAAGATCCCAATAATGTCAAAC8990.24657158529895776No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA8810.24163466812945697No Hit
CAGCATGAGAACAGCAGTTTATTACACTGACGGGGTGAAGTGGAGATGTA8640.2369720241360395No Hit
TTTGAAACAGAAGCAGAAGATGCCAGCCTCCGAGACTGTAGAACCATGTC8360.22929237520570486No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA8210.2251782775644542No Hit
AACGTGGCCATCACAATAGACGGCAGATACATTGGACCACTGCTTTTTGT8160.2238069116840373No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT7850.21530444322545253No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC7810.214207350521119No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT7630.2092704333516182No Hit
AATATGACCTACCATTCCATGACTTGAAGTGTTCAATTACAACTTCTTCT7410.20323642347778387No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT7330.20104223806911684No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA7200.1974766867800329No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC7160.19637959407569938No Hit
ACACCGGATATCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTG6790.18623148656061436No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA6330.17361492046077895No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT6160.1689522764673615No Hit
GCAGATGTCTTTGAGTGAGTAGAAGGGGTGGGGGAGATGTAGAACCATGT6090.16703236423477782No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT6060.1662095447065277No Hit
ACACCGGATATCTGCTTCCTCACAGAGGTGGCGGAAACAGGACATGGCAG6040.16566099835436096No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTAGAACACAGAGGAGAATTTATTA5650.15496434448710916No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT5510.15112452002194185No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT5380.14755896873285793No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT5370.14728469555677454No Hit
GTGTCAATGGCACTGAACCCTGTCGTCAGTGTATGCCTTTGTTGCAGACC5280.14481623697202414No Hit
ATTAGGATGTCTGGCACATGCACTGTAGAACCATGTCGTCAGTGTGGAAA5150.14125068568294022No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT4990.13686231486560616No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT4930.13521667580910585No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG4800.13165112452002192No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT4680.1283598464070214No Hit
TGATGACTAGTTAATTTCTTGCAGATGTTACGGTAGCAGTAGGGGGGCTT4490.1231486560614372No Hit
TAATGATCAAGGGGAGAAGGTGCCATGCTTCATGCAGTGTTAGTAAAACA4280.11738891936368624No Hit
ATGACATGCTTCTTGAGCTTTCGGTGTTCTGAAACAACTGTAGAACCATG4260.11684037301151948No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT4080.11190345584201865No Hit
CCTGGAGTCGATTGATTAGAGCCATGTCGTCAGTGTGACACCTACCTGAT4080.11190345584201865No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC4050.11108063631376851No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT4010.109983543609435No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAAATACAACAGCAAATCAA4010.109983543609435No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA3750.10285244103126714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)