FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88182
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC75168.523281395296092No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC66157.501530924678505No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC58566.640811049874124No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA57466.516069039032909No Hit
TGGTGATAGATGACTTGCTGCTACACTGACCCATGTGGAATAGTTCCCAG53686.08741012905128No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG49135.571431811480801No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC47065.336690027443242No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC35684.046177224376857No Hit
GGTCCAGCAGCCCTTTCCTCCTCATCCTGCTCTAGCAAAGCTCCACCATC28493.2308180807874622No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT28113.1877253861332244No Hit
GTACAATTTCTACCGCTGGTTCCCCTGCCTGGAGGAGTCCACTCCCTTCT25252.863396157946066No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA23462.660406885758998No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA21612.450613503889683No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA12391.405048649384228No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATTG11681.3245333514776259No Hit
TCATGCTGTTTACATTCACTAAGGCTACGGTAGCAGAGTCACAGTCACAG9921.1249461341316822No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT9901.1226780975709327No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC9201.0432968179447053No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA6610.7495860833276633No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATTGCTTA5950.6747408768229344No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC5670.6429883649724434No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATTGCTT5470.6203079993649498No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC5280.5987616520378308No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA5050.5726792315892132No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG4960.5624730670658411No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC4870.552266902542469No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA4830.5477308294209703No Hit
TCTGGGGTATCAGGTAGGTGTCGTCAGTGTCCAAGAGGGGGAAGAGGGAA4780.5420607380190969No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATT4650.527318500374226No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATTGC4410.5001020616452337No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT3670.41618470889750747No Hit
AACTTGGGCATGGAGCATGTATGTGTACATGTATGTGTGTGCTGAGGTAT3660.41505069061713273No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACAAAGGT3560.40371050781338597No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG3330.37762808736476833No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATTG3320.37649406908439365No Hit
AGAAAACGGCATTTTGAGTGTTACGGTAGCAGAACAGGAGTGCACGGAAG2420.27443242385067246No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT2240.25402009480392823No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCAGAGAC2230.25288607652355355No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATTGC2170.24608196684130548No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA2120.24041187543943207No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGGAGACCAA2110.2392778571590574No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT2090.23700982059830805No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC2060.233607765757184No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATTGCT2000.22680365607493594No Hit
TTGCATAAAAATTAACAGCAAAAATGCCTGGTAGAAGACTTCCTCCAGAC1950.22113356467306253No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT1920.21773150983193848No Hit
GGAAAACCAGCGGAACCTCCTGTAGAACCATGGTCAGGACTTTGAGCTGT1910.21659749155156383No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT1830.20752534530856637No Hit
GCCACAAAGTAAAAATGTTGTGTGTTTACACTGACACAATGGCAACAGTT1750.19845319906556894No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1720.1950511442244449No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT1660.18824703454219682No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATTGCTT1600.18144292485994873No Hit
GTATGAGTTTTAGAGGCTGTTAATTTGCTATACACTGACGACATGGAGGA1590.18030890657957407No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATTGCTTAGTCATC1390.15762854097208048No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATTGCT1380.1564945226917058No Hit
TTTATCAGTGAAGGGCAAAACATTTCCAGCATGTAGAACCATGTCTCAGT1350.15309246785058175No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTGAGAC1280.145154339887959No Hit
GGGAAGCTCTTCATCCTCACTACAAAACTAGTATCTTCCTTTATTTCACC1250.14175228504683496No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG1240.14061826676646028No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT1210.13721621192533623No Hit
ACATGTACATAGGGTTTATATATCATCAGCTATAAGTATCTTCTCTTGTC1170.13268013880383753No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC1170.13268013880383753No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAGACCAAG1120.12701004740196412No Hit
GATTCCCGCGCCCAGAGCGAGGTAATCATCTGCATTAGACCAAGTCTCTG1090.12360799256084008No Hit
GGCTCCATAGGAAAGTAACAGCTACCGTATTGAAAAAGAAGTGAAAGAAG1060.12020593771971604No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA1040.11793790115896668No Hit
ATGAGCAAGTTGGGGTGTGCACACTGACGACATGGTTCTCCATATCTCTC960.10886575491596924No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTATTGCTTAG900.10206164523372117No Hit
CGATGCGCGGGAAGACGTACGGTAGCAGAACTGTCAAATGACCAAGACCA900.10206164523372117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)