FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88182
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG74958.499467011408225No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG58476.630604885350752No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA58346.615862647705881No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA57336.501326801388038No Hit
GGGAACTATTCCACATGGGTCAGTGTAGCAGCAAGTCATCTATCACCATG53396.054523598920414No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT48925.547617427592933No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA46765.3026694790320015No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA35344.007620602844118No Hit
CAATGATGAACACTGCGGGCTGAAGGCGATGGTGGAGCTTTGCTAGAGCA28183.195663514095847No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC27813.1537048377219845No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT23032.6116440997028874No Hit
AGGAAATAATGAGCGAACCCCACTCCAGAGCCAAGTATGTTGGGCACAGA22892.595767843777642No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT21502.438139302805561No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT12361.401646594543104No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA11561.3109251321131297No Hit
AACCTACCTGTGACTGTGACTCTGCTACCGTAGCCTTAGTGAATGTAAAC9631.0920596040008164No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT9191.0421627996643306No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG9171.0398947631035813No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT6490.735977863963167No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC5940.6736068585425597No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG5590.6339162187294459No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT5440.6169059445238257No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG5230.5930915606359575No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT4980.5647411036265905No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG4860.5511328842620943No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT4810.5454627928602209No Hit
TCCCTCTTCCCCCTCTTGGACACTGACGACACCTACCTGATACCCCAGAT4760.5397927014583476No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG4730.5363906466172235No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG4630.5250504638134766No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT4310.488761878841487No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA3630.4116486357760087No Hit
TTGACATACCTCAGCACACACATACATGTACACATACATGCTCCATGCCC3480.3946383615703885No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA3360.3810301422058924No Hit
AGATCCACAGAACTTATAAAAACCTTTGTAGAACCATGTCGTCAGTGTAC3260.36968995940214555No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT3250.36855594112177087No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG2800.3175251185049103No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAATTGGTGGTG2780.31525708194416097No Hit
TCCGTGCACTCCTGTTCTGCTACCGTAACACTCAAAATGCCGTTTTCTTG2430.27556644213104714No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG2240.25402009480392823No Hit
CTGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA2200.24948402168242953No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA2150.24381393028055612No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2140.24267991200018144No Hit
CCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA2110.2392778571590574No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT2080.23587580231793337No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA2010.2279376743553106No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1990.22566963779456126No Hit
GGAGGAAGTCTTCTACCAGGCATTTTTGCTGTTAATTTTTATGCAATGTA1940.21999954639268784No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA1880.21319543671043978No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC1820.20639132702819168No Hit
CAGAACTGTTGCCATTGTGTCAGTGTAAACACACAACATTTTTACTTTGT1760.19958721734594362No Hit
ACTCAGTCATAACAGCTCAAAGTCCTGACCATGGTTCTACAGGAGGTTCC1740.19731918078519425No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1670.1893810528225715No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT1620.1837109614206981No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1620.1837109614206981No Hit
CAGCTGCCTACTTCCTCCATGTCGTCAGTGTATAGCAAATTAACAGCCTC1570.1780408700188247No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT1390.15762854097208048No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1370.15536050441133112No Hit
GGCCTTTTCCTTCAAACAGGATTACACTGAGACATGGTTCTACATGCTGG1330.1508244312898324No Hit
CGGAAATAATGAGCGAACCCCACTCCAGAGCCAAGTATGTTGGGCACAGA1290.1462883581683337No Hit
CAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA1280.145154339887959No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGTAGTGAGGATGAAGAGCTTCCC1270.14402032160758432No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG1230.1394842484860856No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT1210.13721621192533623No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG1160.13154612052346282No Hit
CTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAAC1150.13041210224308816No Hit
CCATGGACAAGAGAAGATACTTATAGCTGATGATATATAAACCCTATGTA1140.12927808396271348No Hit
AATGCAGATGATTACCTCGCTCTGGGCGCGGGAATCTGTAGAACCATGTC1070.12133995600009072No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT1040.11793790115896668No Hit
TTATCACTTCTTTCACTTCTTTTTCAATACGGTAGCTGTTACTTTCCTAT970.10999977319634392No Hit
GTTGAAATTGAGAGAGATATGGAGAACCATGTCGTCAGTGTGCACACCCC960.10886575491596924No Hit
TGGTCATTTGACAGTTCTGCTACCGTACGTCTTCCCGCGCATCGTGTAGA910.10319566351409584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)