FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423940
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA14301810.043822071154683No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG1093477.6791859207550885No Hit
TCTATTGTGATGGCCACGTTTTCCATTATTTTCTAATGGCAAAGATTTCT805045.653609000379229No Hit
TTCTTCTCTTGGAAGGCTAGGATTGACAAATTCTTTAAGTTCACTGGTAT630624.428697838392067No Hit
TTTCCTCTAAAATGTTCCTCTGTTGACTTTTTTTTTCTTTTAGGCATAAT533783.748613003356883No Hit
AGAGAAGTTTCTGAGAGGTTCTTGAACTTGGTTGTCCTGTGCATGTGCCA493643.4667191033330056No Hit
GGGAAGGAAAGAATTTTGCTTAAGATATCAGTGTTTGGCCAACAATACAC487013.420158152731154No Hit
TCAGATATAAAAGAAGAGGTCTTGGCTGCAGCATGTCACCCAGTACAACA468233.288270573198309No Hit
GATGTCAGATACCACAGCATCTTTACATTGATGTTTCTTACCTTTCCACT358332.5164683905220726No Hit
AACCGCAGATTTGCTAAAGGAACTGTCTTTAGTGGAACAAAGAAAGAATG274711.9292245459780608No Hit
TTTCCTCTAAAATGTTCCTCTGTTGACTTTTTTTTCTTTTAGGCATAATT101220.7108445580572215No Hit
AATGGTTTTACCAAGGAAGGATTTTCGGGTTCACTCTGTAGAAGTCTTTT65660.46111493461803166No Hit
GCATTGGAGGAATATCGTAGGTAAAAATGCCTATTGGATCCAAAGAGAGG55040.3865331404413107No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA33730.23687795834094133No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA33160.23287498068738852No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGAGACCAA33020.23189179319353342No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT30830.2165119316825147No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC28980.2035198112280012No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC28870.20274730676854363No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC28480.2000084273213759No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC28170.1978313692992682No Hit
GGTGCATTTGTTAACTTCAGCTCTGGGAAAGTATCGCTGTCATGTCTTTT28080.19719932019607567No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA27970.19642681573661813No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG27490.19305588718625785No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA26460.1858224363386098No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA26420.18554152562607976No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT26400.18540107026981473No Hit
TTTGCAGACCTTCTCAGGAGTCACTACTTCATTCCTGCTCTTAGTGTCTG26140.1835751506383696No Hit
TGCCAGCACTCTTATTTTAACTCCTACTTCCAAGGATGTTCTGTCAAACC26120.18343469528210457No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA25930.18210036939758698No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT25750.18083627119120188No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG25590.1797126283410818No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG25450.17872944084722672No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA25360.17809739174403416No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG25330.17788670870963666No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA24980.17542873997499894No Hit
GCCCTCTCAGAAACATCTGCAGGAGGACTTGGGGAAACAAATTCATATGA24770.17395395873421632No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA24400.17135553464331363No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT24360.1710746239307836No Hit
TTTCCTCTAAAATGTTCCTCTGTTGACTTTTTTTTTTCTTTTAGGCATAA23980.1684059721617484No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG23500.16503504361138813No Hit
GTCCCAAATGGCAATTGTTCCAGAAGTCAAGATTGCTGCTGCACAGTGAT23330.16384117308313553No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT23200.16292821326741297No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA22950.16117252131410031No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT22900.16082138292343778No Hit
CCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAA22850.16047024453277525No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC22490.15794204812000506No Hit
TGCTCAGCCAATGGTAAAGAGACTTCAGTTAAACTTCCAGACCAAGTCTC22450.15766113740747503No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG22310.15667794991361994No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC22210.1559756731322949No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA22210.1559756731322949No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC22180.15576499009789738No Hit
TACTCTTCTTGGCTCCAGTTGCAGGTTCTTTACCTTCCAGACCAAGTCTC22090.15513294099470482No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC22090.15513294099470482No Hit
AGAGGAACGTTACACACCAGCCATAGATGTTTGGAGCTGTGGGTAAGGTT22020.1546413472477773No Hit
CCTACTTCCAAGGATGTTCTGTCAAACCTAGTCATGATTTCTAGAGGCAG21890.15372838743205472No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG21870.1535879320757897No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA21810.1531665660069947No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT21450.1506383695942245No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG21380.15014677584729694No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT21330.1497956374566344No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG21110.14825062853771928No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT21060.14789949014705678No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA21020.14761857943452675No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC20920.14691630265320166No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT19910.13982330716181862No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTACTTACGTT19710.1384187535991685No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT19660.13806761520850597No Hit
GTGATACTGACTTTCAATCCCAGAAAAGTCTTTTATATGATCATGAAAAT19540.13722488307091593No Hit
TTTTGTGCGAAGGAAACCGATGGGGATCGGAACCGTAGCGGTTGAGCTGC19460.13666306164585587No Hit
GGTGGTGTACCTGGTGAAAGACTAATGAATTCATCGGACATGTTACTGTT19410.13631192325519334No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC19350.1358905571863983No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA19320.13567987415200078No Hit
GCAACAGCTTCATTGCTGAGGGACTAGACATCACTCGCCTGTAACTTGAA19290.13546919111760328No Hit
GCAGGAAGGAAAAGGAACCTGTATTCTTGTAGGTGGTCATGAAATCACTT19260.13525850808320575No Hit
AAAGTGAGGCACTCCAGTTTTATTTAAGTATTCCCAATATAAGTTATATA19140.13441577594561568No Hit
CACGTTTTTCCCATCTAGCAATTCCATGCACTTCTCATTTCTGTTGAAGT19090.13406463755495315No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG19040.13371349916429062No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG19030.13364327148615812No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG18980.1332921330954956No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG18930.1329409947048331No Hit
GAGAAAGCCCCCCTACTGCTTTAAATGGAATGAGAAAACAGACCAAGTCT18900.13273031167043556No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA18880.13258985631417053No Hit
AAAGCAGAAGCCTTAGAAACTCTGCAAATTATCAGAAGAGAATGTCTCAC18790.13195780721097797No Hit
GCCACAAAGTAAAAATGTTGTGTGTTTACTTTTTTGCATCTTGGCAGGCT18740.13160666882031546No Hit
ATTTACCCTACCAATTGCCCAGTGCGGGACTACCAGCTGCACATTTCCCG18720.13146621346405046No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA18690.13125553042965293No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT18650.1309746197171229No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG18470.1297105215107378No Hit
AAGGTTTAAATTTTTACTTGCATCCTTATTTTTATTTTTAAACCCTTTTT18360.12893801705128025No Hit
GCCCTGACGGAAGCTACTTCCCAGCAGCAGGACTCAGAGACCAGACCAAG18310.12858687866061771No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT18280.1283761956262202No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA18190.12774414652302765No Hit
AGTCTTCAGAATGCCAGAGATATACAGGATATGCGAATTAAGAAGAAACA18150.12746323581049765No Hit
AGCAAGCTGTGACGGGTAAGCTTTATAAGGAAAGTAAATCTTCACCCCAC18110.12718232509796762No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT18050.12676095902917256No Hit
GATGTCAGATACCACAGCATCTTTCCAGTTCGTATAAATAAGACCAAGTC17940.12598845456971502No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC17870.12549686082278746No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA17730.1245136733289324No Hit
CTTTTTCTGGTTGGGCAGTTGGTGGAATTAATACACTGTCTTCATTAATT17630.12381139654760734No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTTTTTAAGGATAAGCTGTTAAC17590.12353048583507732No Hit
CCAAGGAACATCTTCAGTATCTCTAGGATTCTCTGAGCATGGCAGTTTCT17590.12353048583507732No Hit
TTTGCTAATAGATGCCTAAGCCCAGAAAGGGTGCTTCTTCAACTAAAATA17420.12233661530682474No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC17250.12114274477857212No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT17220.12093206174417462No Hit
CGATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGT17210.12086183406604212No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC17070.11987864657218703No Hit
GCACGGTTTCTGTAGCCCATACTTTGGATGATAGAAACTTCATCTTTTAG17070.11987864657218703No Hit
GCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAG17040.11966796353778952No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG16930.11889545907833195No Hit
GGGATAAAAGCACAAAGGTCTGCTGATTTCTCACCAGCATTCTTATCTAC16750.11763136087194685No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT16720.11742067783754934No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA16590.11650771802182676No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT16560.11629703498742924No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC16500.11587566891863421No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA16490.1158054412405017No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA16400.11517339213730916No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA16300.11447111535598409No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT16250.11411997696532158No Hit
GGTATCAGATGCTTCATTACAAAACGCAAGACAAGTGTTTTCTGAAATAG16130.1132772448277315No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT15970.11215360197761141No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG15970.11215360197761141No Hit
CCAGAGACCAGACTCCAGAGCTGGGAGGCGAGGTCACATTCCACTGGCCC15930.11187269126508138No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG15910.11173223590881637No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG15890.11159178055255138No Hit
TATTTCTGGACAGTCTACGAAAAGCTCTTGCTGGCCATGGAGGAAGTAGG15840.11124064216188885No Hit
AAACGTGATGAGACCCGAGTTATGATCTTCTCTTCATTTCGAGATAGTGT15760.11067882073682879No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC15740.11053836538056377No Hit
TTCTGATGAGCCAAGTCTCTGTGACTGTGATGTACATAAACATAATCAAA15650.10990631627737124No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA15560.10927426717417868No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC15530.10906358413978116No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT15350.10779948593339607No Hit
ACCTGAATGTGACCAGTGTTTTCCACTTTTTCAGGATGGTAGTGCTTTGG15340.10772925825526355No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA15300.10744834754273355No Hit
CTACCACTGGCCACTGTAACAGTGGACGAACTCGCCACGGATCCGCAAGC15260.10716743683020352No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC15230.106956753795806No Hit
TGTCATAATGAATGCCCCATCGATTGGTCAGGTAGACAGCAGCAAGCAAT15190.10667584308327598No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC15110.10611402165821594No Hit
GTCTTACTTAGCATGTAGCTAGGAAGTTTTTAGATGATGAAGCAGAACTT15100.10604379398008344No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGAAAATAATCACTCTATTAAAGTTT15060.10576288326755341No Hit
ATCCTGTTCCAAGTCAAGACCACATTTAGCTGGGACACATACTTCTCTTA14990.10527128952062587No Hit
CCATCATGTGAGTCATCAGAACCTAACAGTTCATCACTTCTGGAAAACCA14800.10393696363610826No Hit
CCTCAGGGCCTCAGAGAAAGGCAAAAGGGCCTAGACTTTGACTTCCTTTT14790.10386673595797576No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC14620.10267286542972316No Hit
TGTTAAGTCTTAGTCATTAGGGAGATACATATGGATACACTCACAAATTC14570.10232172703906063No Hit
GAATCAGGGATCACAACACCCCCCAAAATGAGGAGAGTAGCAGAAACTGA14570.10232172703906063No Hit
ATCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACATTCATCTT14440.10140876722333807No Hit
GTCTCGGAGGCTGGCATCACTGAGGCACTGTACGGTCCTTGCAATGTCAA14300.10042557972948299No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA14240.10000421366068794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)