FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423940
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG1372209.636641993342417No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA1061217.452631431099626No Hit
GGAAGTTTAACTGAAGTCTCTTTACCATTGGCTGAGCAAATAGAGTCTCC774935.442153461522255No Hit
GGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATG618474.343371209461073No Hit
TGGTATTGTGGTGGGGATTCTTCATGGTACACCACACTCTGCACAATTCC600204.215065241512985No Hit
GATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGC507383.563211933087068No Hit
GGAGGCTGTCATTCAGAGTCATTTGGATGTCAAGAAAAAAGGGTTTAAAA487403.422897032178322No Hit
GCCTCTAGAAATCATGACTAGGTTTGACAGAACATCCTTGGAAGTAGGAG484523.40267146087616No Hit
GTTTTCTCATTCCATTTAAAGCAGTATTAACTTCACAGAAAAGTAGTGAA355932.499613747770271No Hit
CCTGTTAGCCATCCTTTTCACAGATGAAAACTGGTGACACATATTTAATG245451.7237383597623495No Hit
CATACATTTTTCTCTAACTGCAAACATAATGTTTTCCCTTGTATTTTACA73840.5185611753304212No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT33880.23793137351292892No Hit
ACACTGTGACGTACTGGGTTTTTAGCAAGCATTTTTTAGAAAACACTTTC33010.23182156551540095No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCTGTAGAA32680.22950405213702824No Hit
GTGTACTGCCCTCCTCCCACCTGTCTCTGACCAACATTGGTCTTTTGTGA31880.2238858378864278No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT31000.21770580221076732No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG28980.2035198112280012No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG28850.20260685141227863No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA28660.201272525527761No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG28070.1971290925179432No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC27800.19523294520836554No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG27530.19333679789878788No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA27350.19207269969240276No Hit
TGGTATTGTGGTGGGGATTCTTCATGGTACACCACACTCTGCACAATTCT26980.18947427560150007No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT26500.1861033470511398No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC26100.18329423992583957No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG25240.1772546596064441No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA25220.17711420425017907No Hit
CAAATACCAGTGAACTTAAAGAATTTGTCAATCCTAGCCTTCCAAGAGAA24910.1749371462280714No Hit
CCAACTGGCCAAGACCTCTTCTTTTATATCTGATGTAGAACCATGTCGTC24690.17339213730915629No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA24400.17135553464331363No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC24260.17037234714945854No Hit
ATTAGGATGTCTGGCACATGCACAGGACAACCAAGTTCAAGAACCTCTCA24130.16945938733373597No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA24040.1688273382305434No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA23940.16812506144921838No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT23860.16756324002415832No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC23700.16643959717403822No Hit
ACCACTGTTGGAACATGAATACCAACATTCTTTCTTTGTTCCACTAAAGA23670.16622891413964072No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA23600.16573732039271316No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT23000.16152365970476285No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA22630.15892523561386016No Hit
GGAAGTTTAACTGAAGTCTCTTTACCATTGGCTGAGCATGTAGAACCATG22450.15766113740747503No Hit
CCAGACACTAAGAGCAGGAATGAAGTAGTGACTCCTGAGAAGGTCTGCAA22400.1573099990168125No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT22180.15576499009789738No Hit
GGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTATGTAGAACCATG22080.15506271331657231No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC22000.15450089189151228No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC21980.15436043653524728No Hit
GCCTCTAGAAATCATGACTAGGTTTGACAGAACATCCTTGGAAGTAGGTG21830.1533070213632597No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA21820.1532367936851272No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA21710.15246428922566962No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT21680.1522536061912721No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG21620.15183224012247706No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC21570.15148110173181453No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA21520.151129963341152No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT21390.15021700352542944No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG21390.15021700352542944No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAACTGATTACCTTGCTT21160.1486017669283818No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG21150.1485315392502493No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT21020.14761857943452675No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG20540.14424765088416647No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC20360.14298355267778137No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT19940.14003399019621612No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA19910.13982330716181862No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC19830.13926148573675856No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG19610.13771647681784344No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA19490.13687374468025337No Hit
AGTCAGTATCACTGTATTCCACTTTTGAGTGTTGTACTGGGTGACATGCT19360.1359607848645308No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG19330.1357501018301333No Hit
CACTCCACCCCCAAGAATATCATCTAGTGCTGAACAGAAGGTGATTATGA19310.13560964647386828No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA19280.13539896343947078No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG19260.13525850808320575No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA19130.13434554826748318No Hit
GTCAATACCTGCTTTGTTGCAGCGTGTCTTAAAAATTTCAAAAAATGTTG19050.13378372684242312No Hit
GTTTTCTCATTCCATTTAAAGCAGTAGGGGGGCTTTCTCTGTAGAACCAT18890.13266008399230306No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG18880.13258985631417053No Hit
TGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACA18770.131817351854713No Hit
TCTGAGCACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATA18630.1308341643608579No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC18400.12921892776381028No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT18370.12900824472941277No Hit
TCAGATTGAGACCTCCTTTCCACCACCATGCGATTACTCACGATGTAATC18350.12886778937314775No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG18250.12816551259182268No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCTGTAGAAC18220.12795482955742515No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT18190.12774414652302765No Hit
GGTCTCTGAGTCCTGGTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG18110.12718232509796762No Hit
CTGTGTCACCAAGGGTTTCGTTGGGGCCATGAAGACCACCTTTCCTGAAG18090.1270418697417026No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT18030.12662050367290756No Hit
TTCTTTGTAAGGCGTGTCTCAATATTTGACATTTTCATTTCTGTTGTAGT17910.1257777715353175No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA17810.12507549475399246No Hit
TCAAAAGGAGATACTTACACAACAGGAAAGACTTTGGGAAACACAACACT17800.12500526707585996No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC17720.1244434456507999No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG17660.12402207958200485No Hit
TATTTATACGAACTGGAAAGATGCTGTGGTATCTGACATCTGTAGAACCA17600.12360071351320984No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG17580.12346025815694482No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA17400.12219615995055973No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT17390.12212593227242721No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA17290.12142365549110215No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT17250.12114274477857212No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG17240.12107251710043963No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA17140.12037024031911457No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA16950.11903591443459696No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA16880.11854432068766942No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA16760.11770158855007935No Hit
ACTTATATTGGGAATACTTAAATAAAACTGGAGTGCCTCACTTTTATTAC16760.11770158855007935No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG16680.1171397671250193No Hit
TGGGAAAAGACTAAAGGAACAGGAAGAAATCAGTAGCAAAAATCCTGCTA16660.1169993117687543No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC16600.11657794569995927No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA16560.11629703498742924No Hit
CGACTCAGAGACCGCTTGCTCAGGAAAGGGCTGCTGGACCGCCTTCGACC16550.11622680730929674No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT16340.11475202606851412No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT16260.11419020464345409No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC16020.11250474036827394No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC16020.11250474036827394No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT15830.11117041448375634No Hit
GTTCTGGAGTACGTATAGCAGTATTTTCTTCTCTTGTGAGCTGGTCTGAA15740.11053836538056377No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT15710.11032768234616626No Hit
AATTACCTCCAGTTGCTCCTTCTGGGTAAAACCCTTCGTGCTTTTCCCTG15590.10948495020857621No Hit
GACAGGTTCATGAGTGCTTCCCCCACATTCCTTCAGGTGACTGGTTCTTC15590.10948495020857621No Hit
ACTTGCTTTCCACTTGCTGTACTAAATCCAGAGAAAGCAGATGAATTTAC15570.1093444948523112No Hit
CATTCCTTGTTGTGCTCAGTACTGACTTTGGTATGTGTGTTCTGCTGTCC15510.10892312878351615No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC15470.10864221807098615No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA15430.10836130735845612No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA15300.10744834754273355No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG14990.10527128952062587No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA14990.10527128952062587No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA14950.10499037880809584No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA14950.10499037880809584No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA14900.10463924041743333No Hit
CCAGAAGTGATTTCATGACCACCTACAAGAATACAGGTTCCTTTTCCTTC14820.10407741899237327No Hit
CTTACCAATACTCCATCCACAGATGAAACTTTCTCCCAAGTTAACCAAGC14750.10358582524544573No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT14690.10316445917665071No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTC14650.10288354846412068No Hit
AAAAGCCCCTAAACCCCACTTCATTTTCATCTGTTAAATAACCAGAAGCA14600.10253241007345815No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT14570.10232172703906063No Hit
TACCATTTATAGCCTGTGAAAGTTGAGTTTCATCATTTAAAAAGTCAAGT14570.10232172703906063No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA14490.1017599056140006No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG14440.10140876722333807No Hit
AGCAAACTTTCATCTTGATCAGTGGACAATACTGGGAGGTCACTGTTATC14440.10140876722333807No Hit
TCACAGCCATTAAGAGGACAAACTTCTACTTGCAACCCATGAATTGCTCT14430.10133853954520555No Hit
CTGGGTATGGTGGCACATCTGTAGTCCTAGCTGCTCAGGAGGCTGAGGTG14380.10098740115454302No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCTGGAAAATAAACATGAC14260.10014466901695296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)