FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534769
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA308735.77314691016121No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT292615.471708345098538No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA222154.154130101034278No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC192893.60697796618727No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA145972.7295897854961675No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG136532.5530649682386226No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA133702.5001449223870496No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG111642.0876303600246087No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGAAGCTGGTGGC109092.0399462197696576No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC106421.990018119973297No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT105951.9812292784361099No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATATCAGGACCA102811.922512337102562No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA84791.5855444126342402No Hit
GCAGCTTCTGCCATCTCTCTCTTCCTTTTTCTTCAGCCACAGGCTCCCAG81921.5318763802688637No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA78471.4673625434533415No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG70491.3181392339496119No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGTCTCTGCTACCGTAAGGGAC69341.2966346216777713No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT66351.2407226297709852No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA64851.2126731355033669No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGTATCTCGAAT63171.1812577019236343No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT59561.1137519190528995No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC53691.0039848981522863No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT47450.8872990019989939No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC42840.8010935562831802No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA42440.7936136911451487No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG40470.7567753553403432No Hit
ACTTCTACCGTGCCCTGATTGGAGGCTGTTAGCATGGAAAAGCTGTGTCT39550.7395716655228707No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA38860.7266688981597662No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT38520.7203110127924394No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC36860.6892695724696083No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG35590.6655210006563582No Hit
TGACAGTTTGACAGTTAAAGGCATTTGGTCTCAGCCGTTACCTGTGTGTG33880.6335445771912733No Hit
GGATGCAGAGCTTCTTCCCACACTGACGACATGGTTCTACTGGGTGCGGA33530.6269996951954957No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA33180.6204548131997181No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG32030.5989502009278773No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG29080.5437861955348945No Hit
AGAAAACGGCATTTTGAGTGTGCTGAGGCATAACTGCACCCTTAGACCAA28860.5396722697089772No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC26300.49180113282557514No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGCATCGAAGT25310.4732884666089471No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT25200.4712315036959884No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG23820.4454259689697795No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG23380.4371981173179448No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCCACCGTATGTGGGGGTTG22980.42971825217991316No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCATGTGGGGGTTGTT22120.4136365421331454No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGGCA21500.4020427511691964No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA21410.40035978151313933No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG20540.38409107483792065No Hit
TGTGCTGTGACTGCTTGTAGTACACTGACGACAGAGTTGAAAGTCAGGGC19750.3693183411903083No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT19380.3623994659376292No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT17440.3261221200181761No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC17080.31939024139394767No Hit
CCCCTGCTCCTATAGCCAAACACTGATGCCGACGAGTACCTCATAGACCA16610.31060139985676055No Hit
CAGCCTCTGGCATTCTGGATCAGAGCAGTCAGAGGACCAGGAGACCAAGT16340.30555249088858927No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT16050.3001295886635164No Hit
GGAATCCTATGGCTTTCCAACCTACAGCAACAGAGGAGGGGGAGACCAAG16030.2997555954066148No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT14810.2769420067356186No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT14720.27525903707956145No Hit
GGGGTCAGAGGCAAGCAGGAGGAGATAACACAGGCCCAAGAGACCAAGTC14510.2713321078820949No Hit
TGTTCATTCATGATCCCACTGCTACCGTAACAAGTGTGAGTGACTGGTGG14170.26497422251476804No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC13400.25057548212405734No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG12680.23711172487560048No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC12430.2324368091643308No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA12340.23075383950827366No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG12060.22551793391165156No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATTC11500.2150461227184074No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGTATCTCGAATCTCG11170.20887523397953137No Hit
TGTGCTGTGACTGCTTGTAGAACCAACAGAGTTGAAAGTCAGGGCAAGAC11080.20719226432347423No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGGCTACCTGTTAAAGAAT10720.20046038569924585No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC10560.19746843964403324No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCGCTAATGAGTTTGT9860.18437867565247798No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA9850.1841916790240272No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC9760.1825087093679701No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACGT9620.17989075656965905No Hit
TCAAATCATCCATTGCTTGGTCGGTAGCAGAAAGTCAGTCCCATGGAGAC8860.16567901280739908No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTACGTATCT8240.15408522184345017No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCCCTCTTTAACAATT8190.1531502387011962No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTGCTAATGAGTTTGTACTGAG7640.14286542413640282No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA7620.14249143087950122No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC7550.14118245448034572No Hit
TCAAATCATCCATTGCTTGGCGGTAGCAGAAAGTCAGTCCCATGGAGACC7140.13351559271386337No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT6990.13071064328710152No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC6680.12491374780512707No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGCATTGAAGT6630.12397876466287312No Hit
AGCTGCTCACCATCGCTACCAGGTCTTGGCCAGTTGGAGACCAAGTCTCT6610.12360477140597155No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA6610.12360477140597155No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGCATCGACGT6460.12079982197920971No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT5960.11144999055667026No Hit
CTGGTGTTGTTGGGCAGTTCGTGGTGAGGCTCCCCTTTCTTGCGGAGATA5650.10565309507469581No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGTGGAGGTGGCTTTAGGTCAG5390.1007911827349753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)