FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534769
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA317015.927980118518463No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC309445.7864236707812156No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA297225.557913790814352No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA204313.8205281158780706No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA136842.558861863720597No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG135872.5407231907608705No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT127632.3866379689174204No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT123182.3034244692568193No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC109972.056401923073327No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC106811.9973109884828775No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG103821.9413989965760918No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA81311.5204695859333657No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT79891.4939160646933536No Hit
TCATTACACCAGTTCGTCCCTTACGGTAGCAGAGACGTGGTAAAAAGATA67291.2583003128453594No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT67071.254186387019442No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG65071.2167870613292842No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA64871.2130471287602684No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA59441.11150795951149No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA55311.0342783519613141No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC47830.894404873880124No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA46480.8691603290392674No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT43500.8134353337609322No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA42870.8016545461685325No Hit
GTTCAAATGAGTAGACACAGCTTTTCCATGCTAACAGCCTCCAATCAGGG40730.7616372676800637No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT40510.7575233418541464No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA40040.7487345003169592No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT39660.7416286284358293No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT39160.7322787970132898No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG36540.6832856803591831No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG34550.6460733512974761No Hit
CACACACAGGTAACGGCTGAGACCAAATGCCTTTAACTGTCAAACTGTCA34230.6400894591870508No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT33370.624007749140283No Hit
TTCTCTTCCGCACCCAGTAGAACCATGTCGTCAGTGTGGGAAGAAGCTCT32910.6154059042315467No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG32160.6013811570977375No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG29090.5439731921633453No Hit
AAGGGTGCAGTTATGCCTCAGCACACTCAAAATGCCGTTTTCTTGTAGAA28930.5409812461081327No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT25660.4798333486047247No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC25120.46973553066838203No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA23910.44710893862583656No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC23150.43289719486357664No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC22580.42223838704188166No Hit
ACAAGTCAACAACCCCCACATACGGTGGCAGAGACTTCCATTTTCTACTT22080.4128885556193422No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT21730.40634367362356455No Hit
TGCCCTGACTTTCAACTCTGTGTTCTACAAGCAGTCACAGCACATGTAGA21650.40484770059595826No Hit
ACAAGTCAACAACCCCCACATGGTAGCAGAGACTTCCATTTTCTACTTTT21560.4031647309399012No Hit
TGCCCTGACTTTCAACTCTGTCGTCAGTGTACTACAAGCAGTCACAGCAC19370.3622124693091784No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT19130.3577245502263594No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC17320.3238781604767666No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAAAGCAGCCACCAAGGCTCA17020.31826826162324295No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTAATGTAGA16930.3165852919671858No Hit
ATGAGGTACTCGTCGGCATCAGTGTTTGGCTATAGGAGCAGGGGTGTAGA16550.30947942008605583No Hit
CCTGGTCCTCTGACTGCTCTGATCCAGAATGCCAGAGGCTGTGTAGAACC16400.306674470659294No Hit
CCCCCTCCTCTGTTGCTGTAGGTTGGAAAGCCATAGGATTCCTGTAGAAC16360.3059264841454909No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC16020.29956859877816405No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT15380.2876008145573135No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG15200.2842348752451993No Hit
GGCCTCAGTACAAACTCATTAGCTAGCAGAGACTTGGTCTGGACCTAATT14320.2677791719415299No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC14200.26553521240012046No Hit
CCACCAGTCACTCACACTTGTTACGGTAGCAGTGGGATCATGAATGAACA14100.26366524611561254No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC14070.2631042562302602No Hit
CTTGGGCCTGTGTTATCTCCTCCTGCTTGCCTCTGACCCCTGTAGAACCA13670.2556243910922286No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA13130.24552657315588597No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG12350.23094083613672445No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC11720.21916004854432475Illumina Single End PCR Primer 1 (100% over 21bp)
GTTCAAATGAGTAGACACAGCTTGAGAAAGAGAGAGAGACAGAGATTTCA11510.21523311934685818No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT11450.21411113957615344No Hit
TGCCCTGACTTTCAACTCTGTTGGTTCTACAAGCAGTCACAGCACATGTA11070.20700526769502348No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA10620.19859041941473798No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT10530.19690744975868085No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG10460.19559847335952532No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAAAACACCA10430.19503748347417296No Hit
TCCAGATGATTCTTTAACAGGTAGCCGGTAGCAGAGACTGGGTCGTAGCC10380.194102500331919No Hit
GGCCTCAGTACAAACTCATTAGCGCAGAGACTTGGTCTGGACCTAATTAC9640.18026474982656063No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC9140.17091491840402118No Hit
CCATGGGACTGACTTTCTGCTACCGACCAAGCAATGGATGATTTGATGTA8850.1654920161789483No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA8420.15745116115556435No Hit
GGCCTCAGTACAAACTCATTAGCACTTGGTCTGGACCTAATTACGAGTGA7720.14436139716400914No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG7560.1413694511087965No Hit
CCATGGGACTGACTTTCTGCTACCGCCAAGCAATGGATGATTTGATGTAG7200.1346375724845681No Hit
CCAACTGGCCAAGACCTGGTAGCGATGGTGAGCAGCTTGTAGAACCATGT6970.13033665003019995No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA6680.12491374780512707No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCCCTGTCGTCA6600.12341777477752075No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA6570.12285678489216839No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC6130.11462893324033369No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT6130.11462893324033369No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT5970.11163698718512105No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC5780.10808405124455606No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAACTAACTAACTTCAGAACACCA5780.10808405124455606No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG5750.1075230613592037No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAACTGCCCAACAACACCAGT5570.10415712204708949No Hit
GCTGACCTAAAGCCACCTCCACTTCTACCATGCCCTGAAACAGAGAAGAC5460.10210015913413081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)