FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357103
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA241556.764154879684573No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC226816.351388814991752No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA180065.042242714286915No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT126293.5365146750377345No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG95622.6776588267250623No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT92432.588328857500497No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA88452.476876419408406No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC80322.2492110119489332No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG79332.2214879180516545No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA65061.821883322178755No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA53101.4869659453995066No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACTGACGACATGGTTCTACATTC45861.2842233193224364No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC44961.2590205066885465No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATATGCGCTGCAT44781.2539799441617685No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA42261.1834120687868765No Hit
CAGAGGGGAAAAATATGACAAAGAAATTTGGGGTAGAGGTATGTGGAGTG41521.1626897561767893No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA34420.9638675676205464No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGATCTGGGTCTTCAGTGAAC33290.9322240362024402No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG32650.9143020361072295No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG32260.9033808172992106No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA31570.8840586609465616No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT29000.8120906293142315No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG28140.7880079416862923No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT27410.7675656603276926No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA26960.7549642540107476No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG26760.7493636289809943No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA26360.7381623789214876No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC25780.721920566335203No Hit
ACTTCTACCGTGCCCTGATGGATAAAGAAGACATGGACGACGTGGTGGAT25320.7090391287667703No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC23930.6701147848099849No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT23780.6659143160376698No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC23560.6597536285049411No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT22080.6183090032847666No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG21220.5942263156568273No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG20520.5746241280526907No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC19900.5572621904604554No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC19570.5480211591613624No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG19260.5393401903652448No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGAACTCTG19170.5368199091018557No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACAACAATAATGAGTC18800.5264587527968121No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT18410.5155375339887931No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT18060.5057364401867248No Hit
AGAAAACGGCATTTTGAGTGAGGCATAACTGCACCCTTAGACCAAGTCTC17930.5020960339173852No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA15760.44132925234456166No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTTTTCAGCCACAGGCTCCCAG15670.43880897108117267No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC15080.4222871272434004No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG14860.4161264397106717No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT12830.3592800956586755No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT12730.35647978314379886No Hit
CTGGTGTTGTTGGGCAGTGTATCTCCCCTTTCTTGCGGAGATAGACCAAG12080.33827775179710057No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCCTGCTACCGTATGTGGGGGTTGT11770.3295967830009829No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCTCTCAGGGCAACTG11560.3237161267197419No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC10950.3066342203789943No Hit
CCCCTGCTCCTATAGCCAACACACAGGTGGCCAACACACAGATGCCGACG10390.290952470295685No Hit
TTCTTATTCTGAGGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTAC10280.28787212652932065No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG10230.28647197027188237No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC9730.27247040769749903No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGGCTAATGAGTTTGTA9180.2570686888656774No Hit
ACTTCTACCGTGCCCTGACCAAGTAAGCTGTGTCTACTCATTTGAACAGA8940.2503479388299734No Hit
ACTTCTACCGTGCCCTGATAGCTGGTAAAGCTGTGTCTACTCATTTGAAC8940.2503479388299734No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT8870.24838772006955975No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT8640.24194700128534344No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTGCTAATGAGTTTGTACTGAG8630.2416669700338558No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC8570.23998678252492978No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT8470.23718647001005314No Hit
AGATGAGTCATATTTGTGGGTTTTCAGATGGTATAGGTTCACCACTGACT8430.23606634500410248No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG8390.2349462199981518No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATATGCGCTGCATCTCG7960.22290487618418214No Hit
GGGGTCAGAGGCAAGCAGGCCAGTGTGCAGGGTGGCAAGTGGCTCCTGAC7740.2167441886514535No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG7270.2035827198315332No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTTGTGGGGGTTGTTGACTT7230.20246259482558254No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATAT7220.20218256357409486No Hit
TCCCTGCCAGCGAGATGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTG7200.20162250107111954No Hit
TCTTAAATGGCTACGACCCAGTACACTGACGACATGGCTACCTGTTAAAG7160.20050237606516885No Hit
GACCCAAAACCCAAAATGGCATACGGTAGGAGACAGAAGCAGGGAAGACC6990.19574184478987855No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGTGGAGGTGGCTTTAGGTCAG6920.1937816260294649No Hit
TACCCTCCATGAGGCACACCAAGTAAGCCAGAATTTACAGCTCTCCAGAC6710.1879009697482239No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG6570.18398053222739658No Hit
TCAAATCATCCATTGCTTGGACCGGTAGCAGAAAGTCAGTCCCATGGAGA6540.1831404384729336No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA6020.16857881339557493No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACGCTACCTGTTAAAGAATCA5900.16521843837772296No Hit
TGAGCAAGAGGCTTTGGAGTCACTCTCAGCAGGCAAAGACCAGACCAAGT5810.16269815711433397No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT5810.16269815711433397No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTGTGGGGGTTGTT5780.16185806335987096No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG5700.15961781334796962No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT5490.15373715706672864No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA5460.15289706331226566No Hit
GGAATCCTATGGCTTTCCAACCATGTCAGCAACAGAGGAGGGGGAGACCA5430.15205696955780265No Hit
GGAATCCTATGGCTTTCCAACCCTGACGACATGGTTCTACAGCAACAGAG5150.144216094516148No Hit
TCAAATCATCCATTGCTTGGAGACCAAGTCGCAGAAAGTCAGTCCCATGG5040.14113575074978368No Hit
TCTTAAATGGCTACGACCCAGTCACTGACGACATGGTTCTACATCTTAAA4700.13161468819920302No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAGAACGTGGGAGTAGACGGAAG4470.1251739694149867No Hit
TGTGCTGTGACTGCTTGTAGAACCAACAGAGTTGAAAGTCAGGGCAAGAC4410.12349378190606071No Hit
TCAAATCATCCATTGCTTGGTACGGGTAGCAGAAAGTCAGTCCCATGGAG4240.1187332506307704No Hit
GGTCTTCTCTGTTTCAGGGCATTCATTAAAGAGGAGACCATAGCAGAGAT3670.10277146929597344No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT3610.10109128178704743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)