FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357103
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA225226.306863846005214No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC184515.166856621198926No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA173974.871703682130925No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA110183.0853843288911045No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC110153.0845442351366414No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG97452.7289045457473056No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC95572.6762586704676243No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA80702.259852199505465No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT76522.1427991363836205No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA65331.8294441659689222No Hit
TCATTACACCAGTTCGTCCCTCAGAATAAGAATGTAGAACCATGTCGTCA54471.5253302268533169No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTACC52101.45896282025074No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT49361.3822342573431194No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG46091.2906640381066528No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA45271.267701475484664No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC44031.2329776003001935No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA42311.184812225044315No Hit
AACAGAGAATCTCCATTTTAGCACTCCACATACCTCTACCCCAAATTTCT40451.132726412267609No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC37571.0520774118391611No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT34330.9613472863571575No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT32580.912341817346816No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT31960.8949798797545806No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA30550.8554954732948197No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA29440.8244120043796886No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC28110.7871678479318291No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA27110.7591647227830626No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG26990.7558043477652105No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC26520.7426428789452902No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA23790.6661943472891575No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAAAGACAGAGATTTCA23660.6625539410198178No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT22660.6345508158710512No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA22590.6325905971106376No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAATCTA21120.5914260031419506No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA19450.5446607841435104No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT19420.5438206903890473No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG19110.5351397215929298No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC18510.5183378465036698No Hit
CTCGATAATCTGGATGACTCATTATTGTTGTAGCACAACTAAAAGAAAAG18090.5065765339411878No Hit
AAGGGTGCAGTTATGCCTCACTCAAAATGCCGTTTTCTTGTAGAACCATG17950.5026560964203606No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT17930.5020960339173852No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT17930.5020960339173852No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATGCATACCCCATTTCT17460.4889345650974649No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG14410.40352503339372675No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT13750.3850429707955408No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG13240.3707613769696698No Hit
ATCTCCGCAAGAAAGGGGAGATACACTGCCCAACAACACCAGTGTAGAAC12120.3393978768030512No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC11890.3329571580188349No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA11850.33183703301288425No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGGACTTCCATTTTCTACTTT11340.3175554391870133No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC11240.3147551266721366No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG10240.28675200152337005No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT10240.28675200152337005No Hit
ATGAGGTACTCGTCGGCATCTGTGTGTTGGCCACCTGTGTGTTGGCTATA9820.274990688960888No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGACCTAATTACG9300.26042906388352943No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA9050.25342828259633776No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA9010.2523081575903871No Hit
GTTCAAATGAGTAGACACAGCTTACTTGGTCAGGGCACGGTAGAAGTTGT8910.2495078450755104No Hit
GTTCAAATGAGTAGACACAGCTTTACCAGCTATCAGGGCACGGTAGAAGT8900.24922781382402276No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA8820.24698756381212142No Hit
GGCCTCAGTACAAACTCATTAGCACTTGGTCTGGACCTAATTACGAGTGA8790.24614747005765847No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT8710.2439072200457571No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT8680.24306712629129412No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG8570.23998678252492978No Hit
TCTGGATCAGAGTCAGTGGTGAACCTATACCATCTGAAAACCCACAAATA8540.23914668877046677No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC8380.23466618874666412No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC8350.23382609499220114Illumina Single End PCR Primer 1 (100% over 21bp)
GGCCTCAGTACAAACTCATTAGCTAGCAGAGACTTGGTCTGGACCTAATT8320.23298600123773813No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG7950.22262484493269447No Hit
AGGGATGCAAAGGCCTCATCATCTCGCTGGCAGGGATGTAGAACCATGTC7640.21394387613657687No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA7260.20330268858004555No Hit
TCCAGATGATTCTTTAACAGGTAGCCATGTCGTCAGTGTACTGGGTCGTA7050.19742203229880456No Hit
TCCCTGCTTCTGTCTCCTACCGTATGCCATTTTGGGTTTTGGGTCTGTAG7030.19686196979582923No Hit
ACAAGTCAACAACCCCCACAACACTGACTTCCATTTTCTACTTTTTCTGA7010.1963019072928539No Hit
GCTGACCTAAAGCCACCTCCACTTCTACCATGCCCTGAAACAGAGAAGAC6890.19294153227500188No Hit
GGAGAGCTGTAAATTCTGGCTTACTTGGTGTGCCTCATGGAGGGTATGTA6720.18818100099971155No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT6650.18622078223929792No Hit
CCATGGGACTGACTTTCTGCTACCGGTCCAAGCAATGGATGATTTGATGT6640.18594075098781024No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC6630.18566071973632256No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC6500.1820203134669829No Hit
TCCAGATGATTCTTTAACAGGTAGCGTAGCAGAGACTGGGTCGTAGCCAT5920.1657785008806983No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA5730.16045790710243263No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC5730.16045790710243263No Hit
ACAAGTCAACAACCCCCACACGGTAGCAGAGACTTCCATTTTCTACTTTT5600.15681750083309298No Hit
CCCCCTCCTCTGTTGCTGACATGGTTGGAAAGCCATAGGATTCCTGTAGA5570.15597740707862998No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT5460.15289706331226566No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC5420.15177693830631497No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGGGTTGGAAAGCCATA5240.146736375779537No Hit
CCATGGGACTGACTTTCTGCGACTTGGTCTCCAAGCAATGGATGATTTGA5040.14113575074978368No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC4510.1262940944209374No Hit
TCCGTCTACTCCCACGTTCTACGGTAGCAGAGAGATGGCAGAAGCTGCTG4510.1262940944209374No Hit
GGTCTTTGCCTGCTGAGAGTGACTTGGTCTGGTCTTTGCCTGCTGAGAGT4500.1260140631694497No Hit
TGCCCTGACTTTCAACTCTGTTGGTTCTACAAGCAGTCACAGCACATGTA4380.12265368815159773No Hit
CCATGGGACTGACTTTCTGCTACCCGTACCAAGCAATGGATGATTTGATG4230.11845321937928273No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA3980.11145243809209107No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA3580.10025118803258443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)