FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793604
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT470975.934571902359363No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA449585.665042010876961No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA302263.8087005609850753No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC254403.2056290038860693No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG254403.2056290038860693No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC228232.8758675611514057No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG202332.549508319010489No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT197772.492048931204984No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA191502.4130422729724144No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA183812.316142559765324No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG167362.1088603384055524No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA142591.796739935786614No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC142381.794093779769255No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC141271.780106955106073No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT132481.6693464246651981No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA132421.66859038008881No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC131531.6573757188723848No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG119611.5071748630299242No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT112601.4188436550219001No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT104251.313627451474539No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG97891.2334867263773872No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT94341.1887540889410841No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT92341.1635526030614765No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG87401.1013049329388462No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT75300.9488359433672209No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC71740.9039772985015195No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT62420.7865383743025488No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG60750.7654951335930766No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT57770.7279449196324614No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC55270.6964430622829522No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA51880.6537265437170175No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG47980.6045836462517831No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA47150.594125029611746No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC45000.5670334322911679No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATATAGAGATCAT42840.5398158275411918No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA41530.5233088542900489No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC38970.4910509523641514No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT34650.43661574286419924No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG32960.41532048729593096No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA32190.4056179152322821No Hit
GTAGAGGAGCCGTCAAATCCATGTCGTCAAGAGGGATAAAACACCATGAA31660.3989395214741861No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG30140.37978639220568444No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC30130.37966038477628644No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA28710.3617673298017651No Hit
ACTTCTACCGTGCCCTGATAGATGAAGAAGACATGGACGACGTGGTGGAT28160.35483692118487303No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC26980.3399680445159047No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG26540.33442371762239104No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG24300.30619805343723067No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA21680.2731841069349449No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC20310.2559210891074138No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC20180.2542829925252393No Hit
GACCCAAAACCCAAAATGGCAGGGAAGGGAGAGATGGGGGTGGGAGGCTG19240.2424382941618238No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG18440.2323576998099808No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAATTGGAGCTGGTGGC17950.22618333576947697No Hit
GCAGCTTCTGCCATCTCTCTCTTCCTTTTTCTTCAGCCACAGGCTCCCAG17610.2218990831699437No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG17510.22063900887596333No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACTAGGACCA17010.21433863740606146No Hit
ACTTCTACCGTGCCCTGGGTCTTAGACCAAGTCTCTGCTACAAGCTGTGT16910.2130785631120811No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG16750.21106244424171247No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG16730.21081042938291641No Hit
GCAACAGATAACTCAGATTGCCTTGGTCATACCAGGACCAGAGGAAACAG14850.1871210326560854No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT14810.18661700293849326No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG14220.17918256460400905No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT14020.1766624160160483No Hit
TCAAATCATCCATTGCTTGGTACGGGTAAGCAGAAAGTCAGTCCCATGGA12950.1631796210704583No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT12930.16292760621166225No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGATGTGGTGGAT12370.15587119016537215No Hit
TGACAGTTTGACAGTTAAAGGCATGATAGTGATCTCAGCCGTTACCTGTG12280.15473712330078981No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC12020.15146093013644082No Hit
ACAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAAGGA11640.14667264781931544No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTCGCACAATATCCTTTTGA9810.12361328823947461No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA9070.11428873846401984No Hit
TGTGCTGTGACTGCTTGTAGATGGCTATGGCGCGGACGCGGGTGCCGGGC8580.10811437442351601No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATATTA8570.10798836699411798No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGTAGG7950.10017590637143967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)