FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793604
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC382024.813735817863821No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA295333.7213774124122354No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA278403.5080468344413585No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA265813.34940348082923No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA265063.3399529236243772No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG210562.6532124334050735No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA208442.6264988583726896No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT196362.474281883659861No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA185842.341722067933125No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG169792.1394801437492754No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG145921.8387004097761603No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT135091.702234363738086No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC134951.7004702597265133No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT131861.66153396404252No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC125711.584039394962727No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT122931.5490093295900726No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA120711.5210356802637084No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT116601.469246626781115No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT103371.3025387976875116No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC102771.2949783519236293No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC93671.1803115911714155No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA87141.098028739774497No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG86571.0908463162988091No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG79140.9972227962560672No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT74320.9364872152862133No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC70610.8897384589795415No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT68150.8587406313476242No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT58420.736135402543334No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT57130.7198804441509872No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG52620.6630510934924723No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA52600.6627990786336763No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA52300.6590188557517351No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT47200.594755066758736No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT45690.5757279449196325No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG43880.5529206001985877No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA39210.4940751306697043No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC34690.4371197725817914No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA34410.4335915645586464No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC33550.4227549256304152No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC32920.4148164575783388No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC32310.40713000438505853No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA32090.4043578409383017No Hit
TTCATGGTGTTTTATCCCTCTTGACGACATGGATTTGACGGCTCCTCTAC31570.3978054546096038No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA30720.38709482311077065No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT30330.3821805333642471No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT29170.3675636715540748No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT27900.35156072802052407No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT26990.3400940519453027No Hit
GGCCTCAGTACAAACTCATTAGCATCAAGATTATGACTCACCGTAGCTCC26110.3290053981582754No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC25070.3159006255008795No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG24320.3064500682960267No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC22030.2775943669638762No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT21700.273436121793741No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGGGAGAGAGACAGAGATTTCA20630.259953326848151No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT18740.23613792269192194No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATT18720.23588590783312585No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA17700.22303315003452603No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT17420.219504942011381No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT17170.21635475627643005No Hit
GTTTCCTCTGGTCCTGGTATGACCAAGGCAATCTGAGTTATCTGTTGCTG17090.21534669684124574No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTTTATACTGCAAATGCTATC16380.2064001693539851No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG15930.20072983503107342No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG15920.20060382760167542No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC15740.1983356938725107No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCACCTCTG14750.18586095836210503No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG14710.1853569286445129No Hit
AACAGAGAATCTCCATTTTAGCACCAGAGACTTTGTCATATTTTTCCCCT14600.18397084692113447No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG14250.17956058689220317No Hit
ATGAGGTACTCGTCGGCATCCACCATGTCGTCCATGTCTTCTTCATCCAT13670.172252155987117No Hit
TCATTACACCAGTTCGTCCCTTCCAGCTTTACAGTGAATTGCTGCAACAT13080.1648177176526328No Hit
CCATGGGACTGACTTTCTGCTTACCCGTACCAAGCAATGGATGATTTGAT13070.16469171022323478No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT12880.16229756906467205No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA12490.1573832793181486No Hit
CACACACAGGTAACGGCTGAGATCACTATCATGCCTTTAACTGTCAAACT12260.15448510844199376No Hit
TCCCTGCTTCTGTCTCCTACAGCCACTTGAAGTCCAAAAAGGGTCAGTCT12220.1539810787244016No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA11970.15083089298945065No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT11910.15007484841306243No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT11710.1475546998251017No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT10720.135079964314696No Hit
ATTCAAATGAGTAGACACAGCTTGTAGCAGAGACTTGGTCTAAGACCCAG10060.12676347397442553No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGGGACAGAGATTTCA9430.11882500592234918No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTCCA8920.11239862702304927No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTTTTT8730.11000448586448656No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA8410.10597224812374938No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATGGCGCCTCTG8190.10320008467699256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)