FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938565
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA368193.9229035815313806No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG317353.3812255943914376No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA313053.335410973134519No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT260822.7789231433092008No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG227272.421462551874404No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT214652.2870019657668887No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC204652.180456334936845No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA202502.157549024308386No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG201472.146574824332891No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT178681.9037573316712213No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC178211.8987496870222096No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG176291.878292925902841No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA175711.8721132793146984No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA173731.8510172444103499No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT168881.7993426134577786No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT163031.7370134194222029No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT158421.687895883609553No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG155781.6597678370704214No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC155241.6540143730055987No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC155121.6527358254356386No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC150851.6072408410712096No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA146471.5605738547676506No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG142601.5193406956364237No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG141201.5044243073202175No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG135001.4383660162055905No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG134981.4381529249439302No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT134271.4305881851549973No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT132061.4070416007415576No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA123371.3144534475502496No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC119751.275883929189774No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG117131.2479689739123023No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG114471.2196278361115107No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA113911.2136612807850282No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG112801.2018347157628932No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC112671.2004496225621026No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT110961.1822303196901651No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT95281.015166770548657No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTGGTTGGAGCTGGTGGC93570.9969474676767192No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC90190.9609350444561645No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG80810.8609952427375835No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC73060.7784223788442995No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT69910.7448605051328357No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT66730.7109789945288819No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA64830.6907353246711736No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAATGCTAGAGAAT64260.6846622237138611No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA64250.684555678083031No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC60270.6421505170126736No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA38510.41030722432649847No Hit
GCCTTCTTTAAGCAATGCCATCTTTATCATTTCTTCCAGTGTTCTAATTG24510.2611433411644372No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA22220.2367443917043572No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTGGGGGTG19010.20254324420791314No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC16350.17420210640712153No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA13760.14660678802214017No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG10080.1073979958766841No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAATGCTAGAGAATCTCG9470.10089871239605142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)