FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717932
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG281673.923352072341113No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC211322.943454254720503No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA186712.60066412975045No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA176882.463743084303249No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT162802.267624231821398No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG155472.1655254258063437No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC149102.0767983597332336No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA140851.9618849696071492No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT140621.958681323579392No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG140051.9507418529888625No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG138641.9311021099491317No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT137161.9104873442053008No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC135561.8882011109687267No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT132741.8489216248892653No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA127311.7732877208426427No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT126261.7586623802811407No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA125801.7522550882256258No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC123751.7237008518912653No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA121211.6883214566282043No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT117771.6404060551695705No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT117681.6391524545500131No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA115521.6090660396806382No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT114561.5956942997386938No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG112821.57145802109392No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC110761.5427644958018307No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG109261.5218711521425428No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG102701.4304975958725896No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC100451.3991575803836576No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG99841.3906609539622137No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT97221.3541672470373238No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG96811.3484563997704517No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG90751.264047291386928No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG87131.2136246886891795No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT81731.1384086515157423No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA80101.1157045514059827No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC79341.10511859061861No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC78541.0939754740003231No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG70710.9849122200988394No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG66930.9322609940774336No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATTCTGTTGCCAT66180.9218143222477895No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC63760.8881063944774715No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT51430.7163631095981235No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT46180.6432364067906152No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC43870.6110606575553117No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTGTTCATACCAGGACCA38810.5405804449446465No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA36340.5061760723856856No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA32650.45477844698383685No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA23450.32663260587353676No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC20580.28665667500543224No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG17180.23929842937771267No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA15250.21241566053609534No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC13850.19291520645409313No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATTCTGTTGCCATCTCG13120.18274711253990628No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA10290.14332833750271612No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT9320.1298173086030432No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG8140.11338121159106991No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)