FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269991
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA218658.098418095417996No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA128264.750528721327748No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA81823.03047138608324No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAAACAATAATG76872.8471319414350846No Hit
CAGAGGGGAAAAATATGACAAAGTCTCGTGCTAAAATGGAGATTCTCTGT76762.8430577315540146No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC70632.6160131263634714No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC60392.2367412247074903No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTCCGAAACGTAGCTTGCAAATAT59282.195628743180328No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG58232.1567385579519316No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCAGGGACGAACTGGTGTAATG52931.9604357182276446No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA49641.8385798045120023No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG46461.72079810067743No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCTCTGTGCAAT46181.710427384616524No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA45421.6822782981654942No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG39261.4541225448255681No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT38901.440788767032975No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA31681.1733724457481916No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG29581.0955920752913986No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC26980.9992925690115596No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTACAGAACGTGGGAGTAGACGGA25410.9411424825271953No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT24750.9166972232407746No Hit
TCAAATCATCCATTGCTTGGTACGGGTAGCAGAAAGTCAGTCCCATGGAG23410.8670659392350115No Hit
AGAAAACGGCATTTTGAGTGTGACTTGGTCTGAGGCATAACTGCACCCTT22560.8355834083358333No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT21960.8133604453481782No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG21700.8037304947201944No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT21670.8026193465708116No Hit
GGGGTCAGAGGCAAGCAGGCCCAAGAGACCAAGTCTCTGCTAGGAGATAA20500.759284568744884No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA17930.6640962106144278No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT17150.6352063587304762No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC16930.627057938968336No Hit
TCCCTGCCAGCGAGATGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTG16500.6111314821605165No Hit
GGGGTCAGAGGCAAGCAGTCACAGCACATGGGAGATAACACAGGCCCAAG16070.5952050253526969No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG15160.5615001981547533No Hit
GGGGTCAGAGGCAAGCAGGACATGTAGAACGGAGATAACACAGGCCCAAG14700.5444625931975511No Hit
TGTTCATTCATGATCCCACTGCTACCGACAAGTGTGAGTGACTGGTGGAG14400.5333511117037235No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA13940.5163135067465211No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT13580.5029797289539282No Hit
GGTCTTCTCTGTTTCAGGGCATATCACTTGAGCCTGGGAGGTGGCTTTAG13510.5003870499387016No Hit
GGGGTCAGAGGCAAGCAGGCCCAAGAGACCAAGACTCTGGGAGATAACAC13440.49779437092347517No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC12670.46927490175598446No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA12080.4474223214847903No Hit
GTGTCACTCGTAATTAGGTCCATGTCTCTGCTACCGTAGCTAATGAGTTT11820.43779237085680633No Hit
GGAATCCTATGGCTTTCCAACCCAAGCCAGCAGCAGCAACAGAGGAGGGG11710.43371816097573623No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGGGGGTTGTTGACTTGTA11430.42334744491483045No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT11320.41927323503376035No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATCT11210.4151990251526903No Hit
TGAGCAAGAGGCTTTGGAGTGCAGAGACCAGACCAAGTCACTCTCAGCAG11030.40853213625639373No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGTTGTAAGGAGGTGGCTTTAG10760.3985318029119489No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC10590.39223529673211327No Hit
GGGGTCAGAGGCAAGCAGACCAAGTCTCTGGGAGATAACACAGGCCCAAG10160.37630883992429376No Hit
TCAAATCATCCATTGCTTGGATACGGGTAGCAGAAAGTCAGTCCCATGGA10020.3711234818938409No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC9930.36779003744569266No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG9620.35630817323540415No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG8740.3237144941868433No Hit
ATACGGCCAGGCATTGAAGTCGTAGCAGAGCAGTCAGAGGACCAGGAGAC8550.31667722257408576No Hit
GGAATCCTATGGCTTTCCAACCAAGCCAGCAGCAGCAACAGAGGAGGGGG8110.30038038304980536No Hit
GGTCTTCTCTGTTTCAGGGCATGACCAAGGGAGGTGGCTTTAGGTCAGCA7770.2877873706901341No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTATGCTACCTGTTAAAG7740.28667622254075137No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCTCTGTGCAATCTCG7150.2648236422695571No Hit
ATACGGCCAGGCATTGAAGTCATGGTTCGACAGGGGCCAGGAGGAGACCA7000.25926790152264334No Hit
TCTGTATCAGGCAAAGTCATAGAACCATGGAGCCATTGTCTTTGAGGCAG6600.2444525928642066No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA6550.24260067928190196No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCGCTAATGAGTTTGTACTGA6470.23963761755021462No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGCTACCGATGCCGACGAGTACCTCA6310.23371149408683992No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC6280.23260034593745718No Hit
ATACGGCCAGGCATTGAAGTCTCTGCTACCGTAAGAGCAGTCAGAGGACC6150.2277853706234652No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5980.22148886444362959No Hit
GGAATCCTATGGCTTTCCAACCTGGTTCTACAGCAACAGAGGAGGGGGAG5510.20408087676996642No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGCTAATG5360.19852513602305263No Hit
ATACGGCCAGGCATTGAAGTACTCTGACGACAGGGGCCAGGAGGAGACCA5150.1907470989773733No Hit
GGAATCCTATGGCTTTCCAACCTGGTTCTGCAGCAACAGAGGAGGGGGAG4990.18482097551399862No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTATCTCTGT4980.18445059279753767No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATCTCTGTGCAATCT4950.18333944464815494No Hit
TCTGTATCAGGCAAAGTCATAGAACCATGTCTCAGGAGCCATTGTCTTTG4930.1825986792152331No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATCT4600.1703760495720228No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT4480.16593145697449174No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA4440.16444992610864806No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACTTCATCTGGACCTGGGTCTTA4440.16444992610864806No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT4350.1611164816604998No Hit
GGGGTCAGAGGCAAGCAGGAAACTCGCACGGAGATAACACAGGCCCAAGA3820.14148619768807108No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGTTGAAAGACATTATGACA3560.1318562470600872No Hit
GGAATCCTATGGCTTTCCAACCGTCAGTGCAGCAACAGAGGAGGGGGAGA3560.1318562470600872No Hit
AGCTGCTCACCATCGCTACCGCAGGTCTTGGCCAGTTGGAGACCAAGTCT3260.12074476556625963No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCACCGTAAACAATAATG3260.12074476556625963No Hit
GAGAAAGCCCCCCTACTGCTACCCGTACCTTCCACTCGGATAAGATGCTA3250.12037438284979869No Hit
GGTCTTCTCTGTTTCAGGGCATACGGTAGGAGGTGGCTTTAGGTCAGCAG3110.11518902481934583No Hit
AGCTGCTCACCATCGCTACCGTAACCCAGGTCTTGGCCAGTTGGAGACCA2900.10741098777366652No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCTCTGTG2900.10741098777366652No Hit
GCGTGGACAACCCCCACGCTGGGAGGGGAGATAAGGAGCCAGACCAAGTC2840.10518869147490102No Hit
ATACGGCCAGGCATTGAAGTACCTGACGACAGGGGCCAGGAGGAGACCAA2810.10407754332551827No Hit
AGCTGCTCACCATCGCTAGTAGCAGGTCTTGGCCAGTTGGAGACCAAGTC2790.10333677789259642No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCCAGAC2770.10259601245967459No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT2760.10222562974321367No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG2700.10000333344444814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)