FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269991
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA209857.772481304932387No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA129164.7838631658092305No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA82293.047879373756903No Hit
AACAGAGAATCTCCATTTTAGCACGAGACTTTGTCATATTTTTCCCCTCT76222.823057064865125No Hit
CTCGATAATCTGGATGACTCATTATTGTTTACGGTAGCACAACTAAAAGA72912.700460385716561No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA70952.627865373290221No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA60492.2404450518720997No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCTACGTTTCGGACTATCAAGAA57492.1293302369338236No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT56252.08340278009267No Hit
TCATTACACCAGTTCGTCCCTGGTGGTAAAAAGATAACCTCAGAATAAGA52381.940064668822294No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT50251.8611731502161184No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG47681.765984792085662No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG45851.6982047549733137No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT45571.6878340389124082No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT39291.455233692974951No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT39251.4537521621091074No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT31931.1826320136597146No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG29661.098555137023086No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA27071.0026260134597078No Hit
TCCGTCTACTCCCACGTTCTGTACGGTAGCAGAGAGATGGCAGAAGCTGC25260.9355867417802816No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC25090.9292902356004459No Hit
CCATGGGACTGACTTTCTGCTACCCGTACCAAGCAATGGATGATTTGATG23600.874103210847769No Hit
AAGGGTGCAGTTATGCCTCAGACCAAGTCACACTCAAAATGCCGTTTTCT22780.8437318280979736No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG22400.8296572848724587No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG21810.8078047046012645No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA21550.7981747539732806No Hit
CTTGGGCCTGTGTTATCTCCTAGCAGAGACTTGGTCTCTTGGGCCTGCTT20450.7574326551625795No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT19480.7215055316658703No Hit
AACAGAGAATCTCCATTTTAGCACCAGAGACTTTGTCATATTTTTCCCCT17280.6400213340444681No Hit
AGGGATGCAAAGGCCTCATCATCTCGCTGGCAGGGATGTAGAACCATGTC16720.6192799019226567No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT16650.6166872229074303No Hit
CTTGGGCCTGTGTTATCTCCCATGTGCTGTGACTGCTTGCCTCTGACCCC15870.5877973710234786No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC15270.5655744080358234No Hit
CTTGGGCCTGTGTTATCTCCGTTCTACATGTCCTGCTTGCCTCTGACCCC14700.5444625931975511No Hit
CCACCAGTCACTCACACTTGTCGGTAGCAGTGGGATCATGAATGAACATG14310.5300176672555752No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC13970.517424654895904No Hit
CTTGGGCCTGTGTTATCTCCCAGAGTCTTGGTCTCTTGGGCCTGCTTGCC13840.512609679581912No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT13490.4996462845057798No Hit
GCTGACCTAAAGCCACCTCCCAGGCTCAAGTGATATGCCCTGAAACAGAG13430.4974239882070143No Hit
GGCCTCAGTACAAACTCATTAGCTACGGTAGCAGAGACATGGACCTAATT11910.44112581530495465No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTAGCAGAGACTGGGTCGTAG11900.4407554325884937No Hit
CCCCCTCCTCTGTTGCTGCTGCTGGCTTGGGTTGGAAAGCCATAGGATTC11850.4389035190061891No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA11560.42816242022882245No Hit
ACAAGTCAACAACCCCCACACTGACTTCCATTTTCTACTTTTTCTGAGGT11550.42779203751236156No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG11420.42297706219836956No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC11420.42297706219836956No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA11280.41779170416791667No Hit
GGTCTTTGCCTGCTGAGAGTGACTTGGTCTGGTCTCTGCACTCCAAAGCC11090.4107544325551592No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC10760.3985318029119489No Hit
GCTGACCTAAAGCCACCTCCTTACAACTTCTACCATGCCCTGAAACAGAG10760.3985318029119489No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG10640.39408721031441785No Hit
CTTGGGCCTGTGTTATCTCCCAGAGACTTGGTCTGCTTGCCTCTGACCCC10070.3729753954761455No Hit
CCATGGGACTGACTTTCTGCTACCCGTATCCAAGCAATGGATGATTTGAT10000.3703827164609191No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT9520.35260434607079494No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC8860.32815908678437433No Hit
CCTGGTCCTCTGACTGCTCTGCTACGACTTCAATGCCTGGCCGTATTGTA8570.31741798800700766No Hit
CCCCCTCCTCTGTTGCTGCTGCTGGCTTGGTTGGAAAGCCATAGGATTCC8160.30223229663210993No Hit
TCCAGATGATTCTTTAACAGGTAGCATACGGTAGCAGAGACTGGGTCGTA7840.2903800497053606No Hit
GCTGACCTAAAGCCACCTCCCTTGGTCATGCCCTGAAACAGAGAAGACCT7760.2874169879736732No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC7310.2707497657329318Illumina Single End PCR Primer 1 (100% over 21bp)
CCTCCTGGCCCCTGTCGAACCATGACTTCAATGCCTGGCCGTATTGTAGA6980.2585271360897215No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC6950.25741598794033876No Hit
GCCTCAAAGACAATGGCTCCATGGTTCTATGACTTTGCCTGATACAGATG6660.2466748891629721No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCAGCATCTTGTTCTGTTTG6460.2392672348337537No Hit
GGCCTCAGTACAAACTCATTAGCGACTTGGTCTGGACCTAATTACGAGTG6340.23482264223622268No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG6320.23408187680330084No Hit
CCTGGTCCTCTGACTGCTCTTACGGTAGCAGAGACTTCAATGCCTGGCCG6280.23260034593745718No Hit
ATGAGGTACTCGTCGGCATCGGTAGCAGAGACTTGGCTATAGGAGCAGGG6210.2300076669222307No Hit
CCCCCTCCTCTGTTGCTGTAGAACCAGGTTGGAAAGCCATAGGATTCCTG5590.20704393850165376No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC5320.19704360515720892No Hit
CCTCCTGGCCCCTGTCGTCAGAGTACTTCAATGCCTGGCCGTATTGTAGA5310.196673222440748No Hit
CCCCCTCCTCTGTTGCTGCAGAACCAGGTTGGAAAGCCATAGGATTCCTG5030.18630250637984228No Hit
GCCTCAAAGACAATGGCTCCTGAGACATGGTTCTATGACTTTGCCTGATA4960.18370982736461586No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA4650.17222796315432737No Hit
AAGACCCAGGTCCAGATGAAGTCTAAGACCCAGGGCAGCTACGGTTTCCT4450.16482030882510898No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA4280.15852380264527335No Hit
CTTGGGCCTGTGTTATCTCCGTGCGAGTTTCCTGCTTGCCTCTGACCCCT3830.141856580404532No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT3820.14148619768807108No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGGCAGTAAGATACAGTCTA3780.1400046668222274No Hit
CCCCCTCCTCTGTTGCTGCACTGACGGTTGGAAAGCCATAGGATTCCTGT3610.13370816064239177No Hit
GGCTCCTTATCTCCCCTCCCAGCGTGGGGGTTGTCCACGCTGTAGAACCA3580.13259701249300904No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGGTACAGTCTA3380.12518935816379065No Hit
CCAACTGGCCAAGACCTGCGGTAGCGATGGTGAGCAGCTTGTAGAACCAT3230.11963361741687685No Hit
AGCATCTTATCCGAGTGGAAGGTACGGGTAGCAGTAGGGGGGCTTTCTCT3200.1185224692674941No Hit
GCTGACCTAAAGCCACCTCCTACCGTATGCCCTGAAACAGAGAAGACCTG3150.1166705556851895No Hit
CCAACTGGCCAAGACCTGGGTTACGGTAGCGATGGTGAGCAGCTTGTAGA2960.10963328407243204No Hit
CTCGATAATCTGGATGACTCATTATTGTTTACGGTGGCACAACTAAAAGA2950.10926290135597112No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG2900.10741098777366652No Hit
CCTCCTGGCCCCTGTCGTCAGGTACTTCAATGCCTGGCCGTATTGTAGAA2850.10555907419136194No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT2830.10481830875844009No Hit
CCAACTGGCCAAGACCTGCTACTAGCGATGGTGAGCAGCTTGTAGAACCA2740.10148486431029181No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAGGATACAGTCTA2710.10037371616090907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)