FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162124
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC150389.275616195011226No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA142298.776615430164565No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA63143.8945498507315386No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT62273.8408872221262733No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG47152.908267745676149No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC44632.752831166267795No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA42682.6325528607732354No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA40942.5252276035627053No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC38182.3549875404011744No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGAATAAGAAAGGGACGAACT30871.9040980977523376No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG27391.6894475833312772No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAACAATAATGA26891.6586069921788262No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG24991.5414127457995115No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA23111.4254521230662949No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG22081.3619205052922456No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT20061.2373245170363427No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTGCATGATCAT19241.1867459475463227No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC19141.1805778293158324No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC18961.1694752165009499No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG18041.1127285287804398No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA16411.0121882016234487No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTAATCTGCTTGCAAATATCTTCT16241.0017024006316153No Hit
GGTCTTCTCTGTTTCAGGGCATGTAGAGGAGGTGGCTTTAGGTCAGCAGA15230.9394044065036639No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC14160.8734055414374183No Hit
TCAAATCATCCATTGCTTGGTACGGTGCAGAAAGTCAGTCCCATGGAGAC13640.8413313266388689No Hit
TGTTCATTCATGATCCCACTGCTACCGTAACAAGTGTGAGTGACTGGTGG13050.8049394290789765No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT12520.7722484024573784No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT11910.7346228812513877No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG11270.6951469245762503No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC10240.6316153068022008No Hit
TGTTCATTCATGATCCCACTGCAAATATCTCAAGTGTGAGTGACTGGTGG9910.611260516641583No Hit
CCCCTGCTCCTATAGCCAAGGCAGAAGATGCCGACGAGTACCTCATAGAC9890.610026892995485No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG9800.6044755865880438No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG9430.5816535491352298No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG9270.5717845599664454No Hit
CTGGTGTTGTTGGGCAGTGTCTCCCCTTTCTTGCGGAGATAGACCAAGTC8460.5218228022994745No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC7450.4595248081715231No Hit
GGGGTCAGAGGCAAGCAGGCCCAAGAGACGGAGATAACACAGGCCCAAGA7440.458907996348474No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC7360.4539735017640818No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTGTTACGCTTGCAAATATCTTCT6920.42683378154992474No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA6210.38304014211344406No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA5970.3682366583602674No Hit
GTGTCACTCGTAATTAGGTCCATGTCGTGCTAATGAGTTTGTACTGAGGC5970.3682366583602674No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACTG5710.3521995509609928No Hit
GGAATCCTATGGCTTTCCAACCCATGGTTCTACAGCAACAGAGGAGGGGG5490.33862969085391426No Hit
GGAATCCTATGGCTTTCCAACCTGGTTCTACAGCAACAGAGGAGGGGGAG5110.3151908415780514No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTATGGCTACCTGTTAAA5100.3145740297550023No Hit
TCTTAAATGGCTACGACCCAGTCTCTCTACCGTAGGCTACCTGTTAAAGA4860.2997705460018258No Hit
TCAAATCATCCATTGCTTGGTCGGTAGCAGAAAGTCAGTCCCATGGAGAC4690.28928474500999235No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC4500.2775653203720609No Hit
GAGAAAGCCCCCCTACTGCACACTGACGACATGGTTCTACAGAGAAAGCC4460.2750980730798648No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA4290.26461227208803134No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT4130.25474328291924697No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC3990.24610791739656063No Hit
GCGTGGACAACCCCCACGCTTGCTCAGGAGGGGAGATAAGGAGCCAGACC3460.21341689077496237No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGCATTGAAGT3440.21218326712886432No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA3440.21218326712886432No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGACATGGTTCTACAGCAACAG3320.20478152525227603No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACTG3040.18751079420690336No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA3030.18689398238385435No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC2950.18195948779946214No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCGAATAAGAAAGGGACGAACT2840.1751745577459229No Hit
ATACGGCCAGGCATTGAAGTACCTGACGACAGGGGCCAGGAGGAGACCAA2820.17394093409982483No Hit
GGTCTTCTCTGTTTCAGGGCATGTGTGGCAGTAAAGGAGGTGGCTTTAGG2750.16962325133848166No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCCGTCATAGGGACTCTG2660.16407194493104044No Hit
AGCTGCTCACCATCGCTACCGTTAAGGTCTTGGCCAGTTGGAGACCAAGT2470.15235252029310897No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACTGCATGATCATCT2440.1505020848239619No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC2320.14310034294737362No Hit
GCGTGGACAACCCCCACGACACTGACGTCATGGTTCTACAGCAACAGAGG1930.11904468184846166No Hit
TCACAATTGCCAGTTAACGTTTCTGCTTTGCTGTGTGGAGACCAAGTCTC1920.11842787002541263No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCAGGTGCCGGGC1860.1147269990871185No Hit
ATACGGCCAGGCATTGAAGTTAAAAGACAGGGGCCAGGAGGAGACCAAGT1810.11164293997187337No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACTGCATGATCATCTCG1790.11040931632577533No Hit
CCTCAGAAAAAGTAGTGAGTCATATTTGTGGGGGTTGTTGACTTGTAGAC1720.10609163356443216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)