FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162124
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA148219.141768029409587No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA137958.50891909896129No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA69224.269571439145346No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC61633.8014112654511365No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG48993.0217611211171698No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG45352.797241617527325No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA41052.5320125336162445No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT38542.377192766030939No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA38482.3734918950926454No Hit
TCATTACACCAGTTCGTCCCTTTCTTATTCTGTGGTAAAAAGATAACCTC30841.9022476622831908No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT27621.7036342552614048No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGTAGCACAACTAAAAGAA25801.5913745034664823No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG24441.507488095531815No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT23331.4390219831733735No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC21951.353901951592608No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT20091.2391749525054896No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG19751.2182033505218228No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA19181.1830450766080283No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG17951.1071772223729985No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA17321.0683180775209098No Hit
TGCCCTGACTTTCAACTCTGTGTTCTACAAGCAGTCACAGCACATGTAGA16461.015272260738694No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCAGATTACTATCAAGAAAAACC15980.9856652932323406No Hit
GCTGACCTAAAGCCACCTCCTCTACATGCCCTGAAACAGAGAAGACCTGT15260.941254841972811No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG14290.8814240951370557No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA14240.8783400360218104No Hit
CCATGGGACTGACTTTCTGCACCGTACCAAGCAATGGATGATTTGATGTA13740.8474994448693592No Hit
CCACCAGTCACTCACACTTGTTACGGTAGCAGTGGGATCATGAATGAACA13000.8018553699637314No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT12760.7870518862105549No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG12240.7549776714120056No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC10840.6686240161851422No Hit
CCACCAGTCACTCACACTTGAGATATTTGCAGTGGGATCATGAATGAACA9970.6149613875798771No Hit
ATGAGGTACTCGTCGGCATCTTCTGCCTTGGCTATAGGAGCAGGGGTGTA9850.6075596457032888No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC9550.5890552910118181No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC9320.5748686190816906No Hit
ATCTCCGCAAGAAAGGGGAGACACTGCCCAACAACACCAGTGTAGAACCA8620.5316917914682588No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC8440.5205891786533764No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT7670.4730946682786016No Hit
CTTGGGCCTGTGTTATCTCCGTCTCTTGGGCCTGCTTGCCTCTGACCCCT7540.46507611457896425No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT7340.4527398781179838No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCGTAACACTATCAAGAAAAACC6770.4175816042041894No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT6040.3725543411216106No Hit
GGCCTCAGTACAAACTCATTAGCACGACATGGACCTAATTACGAGTGACA5950.3670030347141694No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC5930.36576941106807137No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA5650.3484986800226987No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGGGTTGGAAAGCCATAGGATTCC5510.33986331450001234No Hit
CCCCCTCCTCTGTTGCTGTAGAACCAGGTTGGAAAGCCATAGGATTCCTG5060.31210678246280626No Hit
TCCAGATGATTCTTTAACAGGTAGCCATACGGTAGCAGAGACTGGGTCGT5000.3084059115245121No Hit
CCATGGGACTGACTTTCTGCTACCGACCAAGCAATGGATGATTTGATGTA4700.28990155683304136No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA4610.28435025042560014No Hit
TCCAGATGATTCTTTAACAGGTAGCCTACGGTAGAGAGACTGGGTCGTAG4550.280649379487306No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCAGCATCTTGTTCTGTTTG4330.26707951938022745No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG4060.2504256001579038No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT4020.24795835286570775No Hit
GGCTCCTTATCTCCCCTCCTGAGCAAGCGTGGGGGTTGTCCACGCTGTAG3780.23315486911253117No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA3620.22328587994374674No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCCCTGTCGTCA3410.21033283165971722No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT3240.19984703066788387No Hit
AGCATCTTATCCGAGTGGAAGGGGGGCTTTCTCTGTAGAACCATGTCGTC3180.1961461597295897No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA3100.1912116651451975No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG2980.1838099232686092No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG2950.18195948779946214No Hit
CCTCCTGGCCCCTGTCGTCAGGTACTTCAATGCCTGGCCGTATTGTAGAA2930.18072586415336409No Hit
GCTGACCTAAAGCCACCTCCTTTACTGCCACACATGCCCTGAAACAGAGA2810.1733241222767758No Hit
TCATTACACCAGTTCGTCCCTTTCTTATTCGGTGGTAAAAAGATAACCTC2800.17270731045372678No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA2780.17147368680762873No Hit
CCAACTGGCCAAGACCTTAACGGTAGCGATGGTGAGCAGCTTGTAGAACC2500.15420295576225604No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2460.15173570847005996No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC1900.11719424637931461Illumina Single End PCR Primer 1 (100% over 21bp)
CCACACAGCAAAGCAGAAACGTTAACTGGCAATTGTGATGTAGAACCATG1880.11596062273321656No Hit
CCTCCTGGCCCCTGTCTTTTAACTTCAATGCCTGGCCGTATTGTAGAACC1870.11534381091016753No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGACGTCAGTGTC1760.10855888085662826No Hit
ACAAGTCAACAACCCCCACAAATATGACTCACTACTTTTTCTGAGGTGTA1720.10609163356443216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)