FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568686
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA247154.345983548038812No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA229974.043883619431461No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC215733.7934818159757757No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT194093.412955479825422No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG184723.2481896863998765No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA155742.73859388133346No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA142542.5064798500402685No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA130632.297049689987093No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG128042.251506103543959No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT125922.214227183366568No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG111711.964352911800185No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG107031.8820579370689625No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC106671.8757275543973302No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT104701.841086293666452No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA101461.7841128496217595No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC100671.7702211765367883No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT98801.7373383554369195No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT95741.6835301027280432No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC94091.6545158488163945No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG93251.6397449559159185No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG83831.474099942674868No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT83121.4616150212947039No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT72451.2739895126660408No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT66411.1677797589530954No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA66181.1637353478017747No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT55750.980330094287533No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG54020.9499090886710769No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG51010.896980055777705No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT46070.8101131380058592No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC45710.8037827553342267No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA44560.7835606995776228No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGTACTACGTAT44150.7763510970904858No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG41860.7360828295403791No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT37150.6532603229198538No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC36340.6390169619086807No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG32940.5792300144543737No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC31430.5526775760261374No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG31110.5470505692069085No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA30390.5343898038636435No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA30330.5333347400850381No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC29700.5222565704096812No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG28250.49675919576005034No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC27490.48339505456438175No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA27460.48286752267507904No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC26320.4628213108815761No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGAAGGGCCAGGAA25250.44400600682977953No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC22060.3879117826005915No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGAAT21090.3708549181798039No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG19590.3444783237146685No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTTACAGCAGGGTCTTC17930.3152882258399187No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC17160.3017482406811492No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC17010.29911058123463563No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG16370.2878565675961779No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT15660.2753716462160138No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG15630.274844114326711No Hit
GGATGCAGAGCTTCTTCCCATGTACACTGACGACATGGTTCTACTGGGTG15630.274844114326711No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG14730.2590181576476298No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG10560.18569122503455335No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT10320.18147096992013168No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA10100.1776024027319118No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC9380.1649416373886468No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA9200.16177644605283056No Hit
AGAAAACGGCATTTTGAGTGTACACTGACGGCATTTTGAGGCATAACTGC8610.1514016522298773No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGAC8570.15069827637747368No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA8480.14911568070956555No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGTACTACGTATCTCG8200.14419204974274027No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC8190.1440162057796394No Hit
TCAAATCATCCATTGCTTGGTAAGCAGAAAGTCAGTCCCATGGAGACCAA7700.13539985158769513No Hit
GTGTCACTCGTAATTAGGTCCAGAGTAAGTTAACTTTTTCCAACAGAGGG7450.13100375251017257No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC7330.12889362495296172No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTAGACCTGGGTCTTCAGTGAAC7220.1269593413588518No Hit
CAGCCTCTGGCATTCTGGGAGCTTCACCTAGACCTGGGTCTTCAGTGAAC6410.11271598034767868No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA6130.10779234938085341No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT5900.10374793822953265No Hit
GGAATCCTATGGCTTTCCAACCCCGACATGGTTCTACAGCAACAGAGGAG5750.1011102787830191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)