FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507085
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA225474.446394588678427No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC219404.326690791484662No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA213914.218424918899198No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG175913.4690436514588288No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT168203.3169981364071113No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA128452.533105889545145No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG122102.4078803356439256No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC111272.1943066744234203No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC106022.0907737361586323No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG105742.085251979451177No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG105532.0811106619205852No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA99211.9564767248094501No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT94021.8541270201248312No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA93501.8438723290966998No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC92611.8263210309908593No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT90431.7833302109113855No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT90331.7813581549444373No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG80671.5908575485372274No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG73661.4526164252541487No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT63711.2563968565427888No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT61071.2043345790153526No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACAGCTGAGTAAT54181.0684599228926117No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC52911.0434148121123679No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT51321.0120591222378892No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT46270.9124702959069978No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC43110.8501533273514302No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA40540.7994714890008578No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG38930.7677213879329896No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT38900.767129771142905No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT38210.7535225849709615No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC36940.7284774741907175No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG34930.6888391492550558No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC30760.60660441543331No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG30620.6038435370795823No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA30190.5953636964217045No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC28660.5651912401273949No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTA28440.5608527170001085No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGAAGGGCCAGGAA27490.5421181853140993No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA27440.541132157330625No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG27110.5346243726396955No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT26990.5322579054793575No Hit
TCCCTGCCAGCGAGATCTCCTCCACCCTGGAGAAAGGAGAACGCCTCTCT25530.5034658883619118No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG23060.4547561059782877No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG22340.44055730301625956No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC21440.42280879931372456No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC20820.41058205231864475No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG19980.3940167821962787No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC19800.3904670814557717No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC19750.38948105347229756No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA18760.36995769939950895No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG15230.3003441237662325No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG14280.28160959208022324No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG13540.26701637792480554No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG12880.25400080854294643No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG12400.2445349399015944No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG11600.2287584921660077No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA10750.21199601644694677No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT10660.21022116607669325No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC10400.20509382056262757No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACAGCTGAGTAATCTCG9480.1869509056667028No Hit
CAGCCTCTGGCATTCTGGGAGCCTCATCTGGACCTGGGTCTTCAGTGAAC9310.18359841052289064No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGATCA9020.17787944821874047No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACC8770.1729493083013696No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC8670.17097725233442126No Hit
GACCCAAAACCCAAAATGGCAGGGGAGAGAGAGATGGGGGTGGGAGGCTG8140.16052535570959503No Hit
TGTGGTCTGCCAGCTAAAGGCACACTGACGACATGGTTTGTACTTTACTT8010.1579616829525622No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA7780.15342595422858102No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC7420.14632655274756698No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAAACATGGACGACGTGGTGGAT7070.13942435686324778No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA6950.13705788970290977No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG6830.13469142254257177No Hit
CAGAGGGGAAAAATATGACAAAGGTGCTAAAATGGAGATTCTCTGTTAGA6800.13409980575248726No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCACCTCTACGGGCCATTCT6710.13232495538223374No Hit
CAGAGGGGAAAAATATGACAAAGTGCTAAAATGGAGATTCTCTGTTAGAC6680.13173333859214925No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGTGGGTGCCGGGC6530.12877525464172673No Hit
TGTGCTGTGACTGCTTGTAGACGGCCATGGCGCGGACGCGGGTGCCGGGC6280.12384511472435587No Hit
ACTTCTACCGTGCCCTGACCCCTGTAGAACAAGCTGTGTCTACTCATTTG6250.12325349793427137No Hit
TACCCTCCATGAGGCACACCACCTGCACTCAGGAAAAGTGGATGAGATGT6220.12266188114418686No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTAGGAGGCTG6200.1222674699507972No Hit
GCAACAGATAACTCAGATTGCCTTTCCTCTGCTACCGTATCATACCAGGA5710.11260439571275033No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACACGGGTGCCGGGC5680.11201277892266583No Hit
ATACGGCCAGGCATTGAAGTCTTATGGAAGCCAGCCCCTCAGGGCAACTG5540.10925190056893813No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA5420.10688543340860014No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT5360.10570219982843114No Hit
TCAAATCATCCATTGCTTGGTACGGTAGGCAGAAAGTCAGTCCCATGGAG5270.10392734945817762No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCTGTAGAACCATGAAGCTGT5210.10274411587800861No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG5190.10234970468461894No Hit
ATACGGCCAGGCATTGAAGTCCCATGGAAGCCAGCCCCTCAGGGCAACTG5080.10018044312097578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)