FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576677
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC250664.34662731477066No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA218833.794671887382365No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA200593.4783769770599484No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG151392.6252130742165893No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC139782.4238872020212354No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA134892.3390910336288773No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA127382.2088621533371366No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT126002.1849319463061643No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG122732.1282277600805997No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC116712.023836567090416No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA113061.960542903566468No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC108901.888405467878899No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG107611.86603592652386No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC105051.8216436584084332No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG101341.7573095511005294No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT98161.7021660305508977No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT91891.5934396551275671No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT89761.5565039007971535No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG86851.5060423772753204No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC86121.4933836445705309No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA77511.3440799615729428No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT76371.3243115296777919No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC68931.1952965004673328No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC66311.1498637885679504No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT61791.07148369017665No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA59821.0373224526034504No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA58131.008016619355376No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT56540.9804448590805598No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT53660.9305035574507046No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT53240.9232204509630174No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT52710.9140298642047455No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT48360.8385976898679851No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG43740.7584835185034257No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG40720.7061145147110081No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT38790.6726469063271121No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT38100.6606818028116259No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC37730.6542657328105681No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA37050.6424740365924079No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG36910.6400463344298455No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA36880.6395261125378678No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA35040.6076191698299048No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT34760.60276376550478No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC32990.5720706738780982No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT30900.5358285487369879No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA30490.5287188495466266No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT30030.5207421138696359No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT28310.49091605872958344No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC24950.4326512068280858No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT22850.3962356743896497No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA22650.3927675284431319No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA22100.3832301270902082No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA20710.35912651276191004No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT18340.3180289832956751No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT17160.2975669222112205No Hit
AAGGGTGCAGTTATGCCTCAAAATGCCGTTTTCTTGTAGAACCATGTCGT16390.2842145603171273No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT16210.2810932289652613No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC15870.2751973808561812No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT14540.25213421031183836No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT14540.25213421031183836No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTTTGTTT11480.19907157733011718No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT10590.18363832786811335No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG10050.1742743338125155No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA9940.17236685354193074No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCTTTGCCTCT9910.1718466316499531No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG9440.16369648867563644No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT8420.14600894434839606No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA6860.11895740596555783No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATATATGGAAGGATGAGAATTT6600.1144488162350848No Hit
CCATGGGACTGACTTTCTGCTACCGCCAAGCAATGGATGATTTGATGTAG6340.10994022650461176No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG6180.10716570974739759No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGATATTTGTCATATTT6070.10525822947681283No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA5920.10265712001692455No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC5890.1021368981249469Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)