FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454249
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA229705.056697978421527No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA212894.686636624406438No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC204414.499954870566584No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT200404.411677295932407No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG125052.7528954384049276No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA122912.7057847127896815No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA119402.6285143170375718No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC114892.5292295635213287No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT113742.5039130520925745No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA112902.4854209915707024No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC111702.459003762253742No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG109412.4085908829738756No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT106942.354215419296465No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG99302.186025725978483No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT94622.082998531642337No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG81311.7899874298017167No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG80731.777219102298519No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT76001.6730911900741663No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT75211.655699847440501No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT70841.559497104011236No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA70671.5557546631913335No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA66541.464835365625461No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG58051.2779334682079653No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG47681.0496445781938981No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC47411.0437007015975819No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA45260.9963698324046943No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC42700.940013076528512No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT42330.9318677641557823No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC40980.9021483811742017No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC37890.8341240156830285No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC37100.8167326730493628No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA35120.773144244676378No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT27800.6119991458429188No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA26520.5838207679048276No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTACTCGCGAAT25350.558063969320791No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG25320.557403538587867No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC23060.5076510900409247No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG22130.48717773732028025No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC22030.48497630154386695No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC20840.4587792158045477No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG18870.4154109310092042No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG18380.40462389570477864No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG17500.38525126087234096No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAAACATGGACGACGTGGTGGAT17430.3837102558288516No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG16990.3740239384126327No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC15410.33924125314530135No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT12380.272537749119976No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGATATGGATGACGTGGTGGAT10770.23709463311972068No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG10500.23115075652340458No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA10060.22146443910718572No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAACTTCAAATGTTA9790.2155205625108696No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC9430.20759539371578142No Hit
GTGTCACTCGTAATTAGGTCCAAGAGACGCTAATGAGTTTGTACTGAGGC9160.2016515171194653No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG8520.1875623281504197No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGCTTGCAAA8000.17611486211307015No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT7480.16466739607572056No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAAACATTATGACA7450.16400696534279657No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAGGAGGTGGCTTTAGGTCAGCAG7310.16092495525581785No Hit
TCCACAAACAGAACAAGATGCTAAAAAGGGTTTGTACTTTACTTTCATTG7280.16026452452289383No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA7190.1582832323241218No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA6940.15277964288308835No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTTATACCAGGACCA6880.15145878141724034No Hit
TCTTAAATGGCTACGACCCAGTTATCATAGCAATTTAGTGAAATAACTAT6560.14441418693271751No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA6390.1406717461128148No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACATGGTGGAT6250.13758973602583605No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAGTATCCTTTTGA6110.13450772593885732No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG5870.12922428007546521No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTGTAGGGACGAACTGGTGT5550.12217968559094242No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA5400.11887753192632235No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG5380.11843724477103967No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT5320.11711638330519165No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGTTCCCAG4970.10941135808774483No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTA4940.10875092735482081No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCTCAG4880.1074300658889728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)