FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246686
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT148736.029122041785914No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA128885.224455380524229No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA117144.748546735526134No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC112204.548292160884687No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT78283.1732647981644684No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA68392.7723502752486966No Hit
AGGACAAAATGTTTCACTTTTGGGTAACGGGTGCAGATAATGACAAGGAA64652.6207405365525407No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCACTGACGACATGGTTCTACA60702.4606179515659585No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA45751.8545843704142109No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC43751.7735096438387263No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG43391.758916193055139No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG41911.6989208953892803No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG40231.630818125065873No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT39411.5975774871699246No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGTATGATGTAT33701.3661091427969159No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCAAGAGGGATAAAACA31861.29152039434747No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA29411.1922038542925013No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGAACAATAATGAG27911.131397809360888No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG27801.1269386993992363No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA27611.119236600374565No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC26101.0580251818100743No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG25611.0381618737990805No Hit
GTAGAGGAGCCGTCAAATCCATGCCGTCAAGAGGGATAAAACACCATGAA25381.0288382802429No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA24420.9899224114866674No Hit
CAGAGGGGAAAAATATGACAAAGAATAGTGCTAAAATGGAGATTCTCTGT22130.8970918495577374No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT21860.8861467614700469No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGGTGCTAAAATGGAGA20900.8472308927138144No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG20090.8143956284507431No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTAGCTACCTGTTAAAGAATCAT18870.7649400452396975No Hit
CAGCCTCTGGCATTCTGGATCAGAGCAGTCAGAGGACCAGGAGACCAAGT18440.7475089790259682No Hit
GGAATCCTATGGCTTTCCAACCTGGTTCTACAGCAACAGAGGAGGGGGAG18120.7345370227738907No Hit
GGATGCAGAGCTTCTTCCCACAGGTCTCTGCTACCGTACTGGGTGCGGAA17780.7207543192560584No Hit
CAGTCAGAGGCGCTATGTGTACACTGACGACATGGAACAGTTTTCTTAAC17260.6996748903464323No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG16660.6753524723737869No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT16400.6648127579189739No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG16290.6603536479573222No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT15130.6133303065435411No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC14620.5926562512667927No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC13990.5671177123955149No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC12440.5042847992995144No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGGCTAATGAGTTTGTA12110.49090746941455937No Hit
TTCTTATTCTGAGGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTAC11970.4852322385542755No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG11070.44874861159530743No Hit
CCCCTGCTCCTATAGCCAAGTCTCGATGCCGACGAGTACCTCATAGACCA10780.4369927762418621No Hit
TCAAATCATCCATTGCTTGGTACGTAGCAGAAAGTCAGTCCCATGGAGAC10730.43496590807747504No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC10720.4345605344445976No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG10710.4341551608117201No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC10400.42158857819252005No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT9700.39321242389110045No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTAGCTAATGAGTTTGTACT9330.3782135994746358No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTGGCGAAGCTTGCAAATATCTTC8670.35145893970472586No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCGCTAATGAGTTTGTACT8180.33159563169373213No Hit
CTGGTGTTGTTGGGCAGTGTCTCCCCTTTCTTGCGGAGATAGACCAAGTC8000.32429890630193847No Hit
TCCCTGCCAGCGAGATGGGATGAGGCCTTTGCATCCCTAGACCAAGTCTC7820.31700218091014487No Hit
GTGTCACTCGTAATTAGGTCCATCTGCTACCGTAGCTAATGAGTTTGTAC7700.3121376973156158No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTAGGCATCTGCCTCACCTCC7670.31092157641698354No Hit
CAGAGGGGAAAAATATGACAAAGTCTGTGCTAAAATGGAGATTCTCTGTT7080.28700453207721555No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCAGATTATCGAGAGACCA6860.2780863121539123No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACGT6420.2602498723073056No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAGCTGTGTCTACTCATTTG5990.24281880609357648No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTTCATCTGGACCTGG5720.23187371800588602No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC5640.22863072894286662No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC5460.221334003551073No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA5300.21484802542503426No Hit
TCAAATCATCCATTGCTTGGTACGGGTAGCAGAAAGTCAGTCCCATGGAG5290.21444265179215682No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC4690.19012023381951143No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4660.1889041129208792No Hit
GGGGTCAGAGGCAAGCAGGCCCAGGAGATAACACAGGCCCAAGAGACCAA4550.1844450029592275No Hit
CCCCTGCTCCTATAGCCAAGTCGTCAGTGTGATGCCGACGAGTACCTCAT4290.17390528850441453No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC4230.17147304670714997No Hit
GGTCTTCTCTGTTTCAGGGCATCGGTAGGAGGTGGCTTTAGGTCAGCAGA4230.17147304670714997No Hit
ATACGGCCAGGCATTGAAGTCTCCGGCCAGAGCAGTCAGAGGACCAGGAG3770.15282585959478853No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGTATGATGTATCTCG3710.15039361779752397No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATAGTTCTACAGCAACA3070.12444970529336889No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACGTATGATGTATCT2680.1086401336111494No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAGGACATGGACGACGTGGTGGAT2510.1017487818522332No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT2510.1017487818522332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)