FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246686
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA126405.123922719570628No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG109024.419383345629667No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA102524.155890484259342No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT102334.148188385234671No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA98663.9994162619686566No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC79733.232043974931695No Hit
TCATTACACCAGTTCGTCCCTCAGAATAAGAATGTAGAACCATGTCGTCA73602.983549937977834No Hit
ACTAGATATTCCTTGTCATTATCTGCACCCGTTACCCAAAAGTGAAACAT64232.6037148439716886No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA45841.8582327331101076No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA44311.7962105672798618No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG42751.7329722805509837No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT40381.6368987295590345No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT39731.610549443422002No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA38801.5728496955644018No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA35191.426509814095652No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG34541.4001605279586196No Hit
TTCATGGTGTTTTATCCCTCTTGACTTGGTCTTTCATGGATTTGACGGCT31151.2627388664131731No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA31081.2599012509830312No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT29801.2080134259747208No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA27861.1293709411965007No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA27411.1111291277170168No Hit
CTCGATAATCTGGATGACTCATTATTGTTCGGTAGCACAACTAAAAGAAA26181.0612681708730938No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT25731.0430263573936096No Hit
TTCATGGTGTTTTATCCCTCTTGACGGCATGGATTTGACGGCTCCTCTAC25151.019514686686719No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT24430.9903277851195446No Hit
AACAGAGAATCTCCATTTTAGCACTATTCTTTGTCATATTTTTCCCCTCT21960.8902004977988212No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT21720.8804715306097631No Hit
AACAGAGAATCTCCATTTTAGCACCGGTAGCAGAGACTTTGTCATATTTT21200.859392101700137No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT19420.7872355950479557No Hit
CCTGGTCCTCTGACTGCTCTGATCCAGAATGCCAGAGGCTGTGTAGAACC18630.7552110780506393No Hit
TCCAGATGATTCTTTAACAGGTAGCTAGCAGAGACTGGGTCGTAGCCATT18520.7507519680889876No Hit
CCCCCTCCTCTGTTGCTGTAGAACCAGGTTGGAAAGCCATAGGATTCCTG18230.7389961327355423No Hit
TTCTCTTCCGCACCCAGTACGGTAGCAGAGACCTGTGGGAAGAAGCTCTG17700.7175113301930389No Hit
TCTAGATATGGTTAAGAAAACTGTTCCATGTCGTCAGTGTACACATAGCG16770.6798115823354386No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT16640.6745417251080321No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG16620.6737309778422772No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG16060.6510300544011415No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA14560.590224009469528No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG13300.5391469317269727No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA12200.49455583211045623No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC11880.48158387585837864No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA11110.4503701061268171No Hit
ATGAGGTACTCGTCGGCATCGAGACTTGGCTATAGGAGCAGGGGTGTAGA10900.44185725983639124No Hit
CCATGGGACTGACTTTCTGCTACGTACCAAGCAATGGATGATTTGATGTA10820.4386142707733718No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG10750.4357766553432298No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG10600.4296960508500685No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG9890.4009145229157715No Hit
GGCCTCAGTACAAACTCATTAGCTAGACTTGGTCTGGACCTAATTACGAG8620.34943207154033873No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCTTCGCCACTATCAAGAAAAAC8240.33402787349099666No Hit
ATCTCCGCAAGAAAGGGGAGACACTGCCCAACAACACCAGTGTAGAACCA7960.3226774117704288No Hit
AGGGATGCAAAGGCCTCATCCCATCTCGCTGGCAGGGATGTAGAACCATG7920.3210559172389191No Hit
GGCCTCAGTACAAACTCATTAGCGAGACTTGGTCTGGACCTAATTACGAG7370.29876036743066087No Hit
GGCCTCAGTACAAACTCATTAGCTACGGTAGCAGATGGACCTAATTACGA7070.28659915844433814No Hit
AACAGAGAATCTCCATTTTAGCACAGACTTTGTCATATTTTTCCCCTCTG7000.2837615430141962No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA6710.2720057076607509No Hit
CTCGATAATCTGCACAACTAAAAGAAAAGTTTAAAAGTGATATATGTAGA6170.2501155314853701No Hit
GTTCAAATGAGTAGACACAGCTGGTCTTCTCTGTTTCAGGGCACGGTAGA6090.24687254242235068No Hit
AAGACCCAGGTCCAGATGAAGACTTGGTCTAAGACCCAGGGCAGCTACGG5740.23268446527164088No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA5700.23106297074013118No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG5440.22052325628531816No Hit
CCATGGGACTGACTTTCTGCTACCCGTACCAAGCAATGGATGATTTGATG5340.21646951995654395No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTAGCAGAGACTGGGTCGTAG5180.20998354183050516No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC5150.2087674209318729No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGCCTCAGTACAA4980.2018760691729567No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC4930.1998492010085696No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG4760.19295784924965342No Hit
CTTGGGCCTGTGTTATCTCCTGGGCCTGCTTGCCTCTGACCCCTGTAGAA4500.1824181347948404No Hit
GCTGACCTAAAGCCACCTCCTACCGATGCCCTGAAACAGAGAAGACCTGT4330.1755267830359242No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGACCTAATTACG4280.1734999148715371No Hit
ATGAGGTACTCGTCGGCATCACACTGACGACTTGGCTATAGGAGCAGGGG4280.1734999148715371No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG4170.16904080490988543No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC3890.1576903431893176Illumina Single End PCR Primer 1 (100% over 21bp)
CCTGGTCCTCTGACTGCTCTGGCCGGAGACTTCAATGCCTGGCCGTATTG3770.15282585959478853No Hit
CCCCCTCCTCTGTTGCTGTAGAACTATGTCGTCAGTGTGGTTGGAAAGCC2970.12039596896459466No Hit
CCTCCTGGCCCCTGTCTCGTGTACTTCAATGCCTGGCCGTATTGTAGAAC2920.11836910080020756No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT2830.11472073810431073No Hit
CCTCCTGGCCCCTGTCGTCAGTGTCACTTCAATGCCTGGCCGTATTGTAG2740.11107237540841394No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2710.10985625450978168No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT2650.10742401271251713No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT2550.1033702763837429No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC2510.1017487818522332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)