FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655296
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA242593.701991161246215No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA223903.4167765406778003No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT205883.141786307256568No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC205593.137360826252564No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA147542.2515016114854967No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG141662.1617711690594783No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA139982.1361338997949018No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG138632.1155325227072956No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA134072.045945648989159No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG133892.0431987987108116No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC133242.033279617150112No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT123501.8846444965328644No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG122011.8619066803398767No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT121941.8608384607871862No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC118011.800865563043266No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC117791.7975083015919522No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT113751.7358567731223753No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT111661.7039627893348959No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG109801.6755786697919721No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC106851.6305608457857212No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT97721.4912344955562067No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT96421.4713961324348082No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA96301.4695648989159098No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT93291.42363145815021No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA86931.3265760816485985No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG84141.2839999023342124No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA83331.2716390760816485No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC72291.1031655923430024No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG71101.0850058599472605No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT70281.0724924309014552No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG58270.8892164762183806No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT54670.8342794706514308No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG54010.8242076862974899No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT51250.7820893153628284No Hit
GACCCAAAACCCAAAATGGCAGGAGAGGGAGAGATGGGGGTGGAAGGCTG50890.7765956148061334No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC50040.7636243773806036No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG49010.7479062896767262No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC48410.7387501220822346No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC47920.7312725852133998No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG45930.7009046293583358No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG44890.6850339388612169No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA37940.5789749975583554No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC37870.5779067780056646No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG35010.5342623791385878No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA33580.5124401797050493No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATAGAGTCTGTAT32140.4904653774782694No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA28690.4378174138099424No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCATAGCAGGGTTTTC25460.388526711592929No Hit
GTAGAGGAGCCGTCAAATCCAGAAGCTAGCAGTTCAACTTCTGTAACACC24310.3709773903701533No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATACTGAGG23710.3618212227756617No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC21900.3342001171989452No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC19870.3032217501709151No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA17810.27178557476316045No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC14630.22325788651235473No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT14050.21440692450434615No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC12800.19533157534915518No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA12690.19365294462349839No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC11770.17961348764527788No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA11630.17747704853989646No Hit
TGTTCATTCATGATCCCACTGCTTTCTTTTCTTGCTTCATCCTCTCAGGG11340.17305156753589218No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTGTGCTAAAATGGAG10410.15885950776443014No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGATAGTTGGAGCTGGTGGC9500.1449726535794511No Hit
GACCCAAAACCCAAAATGGCAGGGGAAGGAGAGATGGGGGTGGGAGGCTG9320.14222580330110363No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATTCTCTCAGGG8870.13535867760523487No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAAGG8730.1332222384998535No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT8540.13032278542826448No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG8480.1294071686688153No Hit
ATACGGCCAGGCATTGAAGTTTCATGGAAGCCAGCCCCTCAGGGCAACTG8110.12376086531887881No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGATATGGACGACGTGGTGGAT7730.11796195917570075No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTAGAGGACCGTCGCTTGGTG7690.11735154800273465No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCTTCTCAGGG7630.11643593124328548No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG7550.11521510889735327No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCACTCATGGTGGGGGCAGCGCCT7500.11445209493114562No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTAGTAGTTGGAGCTGGTGGC7500.11445209493114562No Hit
GACCCAAAACCCAAAATGGCAGGGGAAGGAGAGATGGGGGTGAGAGGCTG7480.11414688934466256No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA7310.11155264185955659No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTACTCCCTGGGGGCAGCTCGTGGTG7140.10895839437445062No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG6610.10087044633264967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)