FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963117
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT438934.557390223617691No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA393014.080604952461643No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC339013.519925408854791No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA317303.2945114664158144No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC296103.074392830777569No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA252812.6249147299860764No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG226512.3518430263405175No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT218812.2718942766039847No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT218032.263795572085219No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC199302.0693228340897316No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT185241.9233384936617255No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGCTGCTCACT182141.8911513346768876No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT179101.8595871529627241No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG177901.8471276075492384No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA170361.768840130534504No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG170021.765309926000683No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA166551.7292810738466873No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC160111.6624148467943147No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA149241.5495521312571578No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC129341.3429313364835218No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC128101.3300564728895867No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA127101.3196735183783486No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG122891.2759612798860367No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT120511.2512498481492904No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG119041.2359869050177705No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT109411.1359990530745485No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG103401.0735974964620083No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG91270.9476522582406913No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC90280.9373731332745658No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT88210.9158804174363032No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC84300.8752830652973627No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG77860.8084168382449899No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA74400.7724918156361066No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC68330.709467281752892No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT67480.7006417704183396No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG60960.6329449070050679No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA60420.6273381115689993No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT57440.5963969071255102No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGAAGCTGTGTCTACTCATTTGAA57240.5943203162232626No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC57020.5920360662307902No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA52980.5500889300053887No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG49620.5152022028476291No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT48440.5029503165243683No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGCGAGACGTAT43840.4551887257726735No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG43170.44823214625014407No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG39790.4131377600021597No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA39420.40929606683300157No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC39360.4086730895623273No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG37260.3868688850887275No Hit
CAGAGGGGAAAAATATGACAAAGTCCTGTAGAGTGCTAAAATGGAGATTC34800.3613268169910821No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC34130.3543702374685526No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA32630.3387958057016956No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACTAGGACCA31750.3296588057318062No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC29000.3011056808259017No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG27980.290515067224439No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGAACCTGGGTCTTCAGTGAAC27070.2810665786192124No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG25040.2599891809613993No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT24830.25780876051403934No Hit
GACCCAAAACCCAAAATGGCGATTCTCTGTTAGAGTAGGAGACAGAAGCA23430.24327262419830611No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC19960.2072437720443103No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT19950.20713994249919793No Hit
CAGAGGGGAAAAATATGACAAAGTCCTGTAGGGTGCTAAAATGGAGATTC18400.19104636300677902No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG16770.1741221471534611No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCTTCCCT16030.166438760815145No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA15990.16602344263469548No Hit
TGTGGTCTGCCAGCTAAAGGCACCGGGTTTGTACTTTACTTTCATTGGGA14060.14598434042800615No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGAACCA13710.14235030634907286No Hit
AGATGAGTCATATTTGTGGGTTTTCATAGACCAAGACCACTGACTCTGAT13470.13985839726637575No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC12320.12791799957845204No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTACAATCCTGTTTGAAGGAAA12160.12625672685665396No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG12030.12490694277019303No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA11470.11909248824389976No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTGCCTGTTAAAGAATCATC11360.11795036324766357No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTTATACCAGGACCA10390.10787889737176272No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACGCC10250.1064252837401894No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTTAAATGTTA10010.10393337465749229No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGATCA10000.10382954511237992No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAAGACCA10000.10382954511237992No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATGC9990.10372571556726752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)