FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963117
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC393714.087873020619509No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA311483.234082671160409No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA278352.8900953882030946No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA265542.757089740914136No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA248722.582448446035113No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA231152.4000199352726614No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC223492.3204865037165785No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT220572.2901682765437634No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA216132.2440679585138668No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG184621.9169010618647577No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG183361.903818539180598No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC181311.88253348243256No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATAGTGAGCAGCTTATC180911.8783803006280648No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC165691.7203517329670226No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT163921.7019739034821315No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT156281.6226481310162733No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT147631.5328355744940645No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT132741.378233381821731No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA128991.3392973024045884No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA128011.3291220069835752No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT121791.2645400299236749No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG107301.1140910190558364No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC105361.0939480873040346No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA98231.0199176216389079No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT91480.9498326786880513No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA90760.9423569514399601No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT90470.9393458946317009No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT89520.9294820878460249No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC88850.9225255083234956No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG88680.920760406056585No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA87210.9054974629250652No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT81840.8497409971997171No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT78490.8149580995870699No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA77630.8060287587074052No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT76820.7976185655533023No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT71900.7465344293580115No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG69040.716839179455871No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT63430.6585908046478257No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT60860.6319066115539441No Hit
GTTCAAATGAGTAGACACAGCTTCTTCTCTGTTTCAGGGCACGGTAGAAG57400.5959815889450606No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC55770.5790573730917428No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC50560.5249621800881928No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC50180.5210166573739224No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG49850.5175902823852138No Hit
GGCTCCTTATCTCCCCTCCCCGTGTCTCCCTTCCCTGATTACCTTTGCGA47000.48799886202818554No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT45370.47107464617486766No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG45100.4682712484568334No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTCGAC44260.45954956666739344No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT43220.44875129397570596No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC40890.42455900996452145No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA39410.4091922372878892No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA39400.4090884077427769No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC38580.4005743850435617No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC35100.36444170334445347No Hit
AACAGAGAATCTCCATTTTAGCACTCTACAGGACTTTGTCATATTTTTCC34180.3548893851941145No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG33110.3437796238670899No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG26450.2746291468222449No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAACGCCTCTG25820.26808788548016493No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT25420.26393470367566974No Hit
GGCCTCAGTACAAACTCATTAGCTAGCAGAGACTTGGTCTGGACCTAATT24290.25220196507797077No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC24200.2512674991719594No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG22630.23496626058931575No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAAGGC22130.22977478333369672No Hit
TCCCTGCTTCTGTCTCCTACTCTAACAGAGAATCGCCATTTTGGGTTTTG22110.22956712424347198No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTTTG21890.22728287425099963No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTTC21410.22229905608560535No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC20330.21108546521346835No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGTGCCTCTG20070.20838589704054647No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT20040.20807440840520933No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA20000.20765909022475984No Hit
AACAGAGAATCTCCATTTTAGCACCCTACAGGACTTTGTCATATTTTTCC18110.18803530619852002No Hit
AAGGGTGCAGTTATGCCTCAGATTTACTTTTATCACCTTTCCTTGCCTCT17020.1767178857812706No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA17000.17651022669104585No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA16470.1710072608000897No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA14070.14608816997311855No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCGGTGCCTTTAGCTGGCAGACCA13510.14027371544682524No Hit
TCTGGATCAGAGTCAGTGGTCTTGGTCTATGAAAACCCACAAATATGACT12760.13248649956339675No Hit
GGCCTTTTCCTTCAAACAGGATTGTACGGTAGCAGCATCTTGTTCTGTTT12190.1265682154919911No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG11980.12438779504463113No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT11980.12438779504463113No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATATACATAGCGCCTCTG11860.12314184050328256No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT11430.11867717006345023No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA11080.11504313598451694No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA10870.11286271553715696No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG10740.11151293145069603No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA9950.10331039738681802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)