FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483627
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT622934.19869684226561No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA537713.6242937072458234No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG449383.0289284301242834No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC423912.8572545525256685No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA361292.4351808102710453No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC349482.355578592193321No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA325732.1954979250175413No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG307172.070399096268806No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT306032.0627152242443687No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT274381.8493866719869616No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA269261.8148766502631726No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG265451.7891963411288687No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA256871.7313650937870504No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG255461.7218613573357724No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG248441.674544882237921No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC242191.6324183908758738No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC239661.615365587172517No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT239131.6117932607050154No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT238851.6099059938919957No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG234131.5780920676153776No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC228441.53974010987937No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA219041.476381866870851No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC218941.4757078430090582No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA213531.43924315208607No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT212831.4345249850535209No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT210961.4219207388379962No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC190881.286576747390011No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG187371.2629185098410853No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA184881.2461353156824457No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT184861.2460005109100871No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGTGCCT179911.212636329751346No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT178741.2047502505683707No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG158631.0692040519618475No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG157491.06152017993741No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG147610.9949266223922859No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG143130.9647303533839704No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC141440.953339350119673No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC125930.8487982491556165No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA115040.7753970506063855No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG108650.7323269258378285No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG105310.7098145288539505No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC102330.6897286177725264No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACAGTCTACATAT89790.6052060255037149No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT85640.5772340352393155No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT72730.49021755468187084No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA71830.484151339925736No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT66240.4464734060515211No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA54920.37017390489658114No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC41370.2788436716236628No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA37900.25545504361945426No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG30230.2037574134199499No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG22640.15259900230987977No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG19930.13433295565529613No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC18190.12260494046010216No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA16420.11067471810637042No Hit
GCCTTCTTTAAGCAATGCCATCTTTATCATTTCTTCCAGTGTTCTAATTG15420.10393447948844284No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACAGTCTACATATCTCG15260.10285604130957444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)