FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961058
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT552855.752514416403589No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA436374.540516805437341No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC321113.3412135375804586No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA316583.294077984887488No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC287832.994928505875816No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA250782.6094158729233823No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG244592.54500768944226No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT197762.0577322076295084No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA187011.9458763154773178No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGCTGCTCACT184681.9216322011782847No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC183341.9076892341565233No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT182091.8946827350690594No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT176831.8399513869090107No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG176141.8327717994127306No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC157931.6432931207065546No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA157881.642772860743056No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG148801.5482936513717174No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC144531.5038634504889403No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT140481.461722393445557No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC134071.3950250661250414No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG133731.3914872983732511No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG121151.2605898915570133No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT113561.1816144290979316No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA107641.1200156494197022No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG106491.108049670259235No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG103371.075585448536925No Hit
CAGAGGGGAAAAATATGACAAAGAAAACTATATAAGATATTATTTTATTT102671.068301809047945No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA90130.9378206102025061No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT88420.9200277194508552No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC71600.7450122677299392No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC69870.7270112729928891No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG69300.7210803094090055No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG66020.6869512558034999No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG61860.6436656268404196No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT57820.6016286217897359No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG53190.5534525491697692No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT52590.5472094296077864No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA52540.5466891696442879No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG52460.5458567537026902No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT51370.5345150864984215No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA49030.5101669202066889No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG48860.5083980363307937No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT44870.46688129124360866No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC44270.4606381716816259No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC40490.42130651844113465No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGATGGGAGGCTG40120.41745659471124535No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGACGCTGATAT37640.39165170052171666No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC36390.3786452014342527No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA34880.3629333505365961No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA33310.34659718768274134No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA33260.34607692771924276No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA32590.33910544420836203No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC32220.3352555204784727No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG29810.31017899023784207No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC28460.29613197122338086No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC25820.2686622451506569No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATAAC24080.25055719842090696No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA23460.2441059748735248No Hit
TCAAATCATCCATTGCTTGGAGACCAAGTTTCTGCTACCGTAGCAGAAAG22360.23266025567655646No Hit
GGAATCCTATGGCTTTCCAACCTGGTTCTACAGCAACAGAGGAGGGGGAG22150.23047516382986252No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAGGACATGGACGACGTGGTGGAT21730.2261049801364746No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATAGTGGGGGCAGCGCCT21250.22111048448688841No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATCTAGTGAAATAACTAT20390.21216201311471317No Hit
GGAATCCTATGGCTTTCCAACCCTGACGACATGGTTCTACAGCAACAGAG20120.20935260931182093No Hit
TCCACAAACAGAACAAGATGCTAAATCCTGTTTGAAGGAAAAGGCCAGAC20050.20862424536292296No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC17970.1869814308813828No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC16390.17054121603482827No Hit
GGTCTTCTCTGTTTCAGGGCATGGGGAGGTGGCTTTAGGTCAGCAGACCA15940.16585887636334123No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA15290.15909549683785995No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGTCT15280.15899144484516023No Hit
CTGGTGTTGTTGGGCAGTCTCCCCTTTCTTGCGGAGATAGACCAAGTCTC13910.14473632184529966No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG12320.1281920550060454No Hit
GGGGTCAGAGGCAAGCAGGGAAGACGGAGATAACACAGGCCCAAGAGACC10840.112792360086488No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTACTAGGCATCTGCCTCACCTCC10180.105924928568307No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACGA10080.10488440864130988No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA9710.10103448491142054No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA9700.10093043291872082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)