FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961058
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC364243.789989782094317No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA360173.7476406210655338No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA315963.287626761340107No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA275332.8648635150011756No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT240192.4992248126543872No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA234362.438562500910455No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA230312.3964214438670717No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA207572.1598072124679257No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATAGTGAGCAGCTTATC190811.9854160727032082No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG181221.8856302117041843No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC179541.8681494769306326No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC177381.845674246507495No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA166411.7315292105159106No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG164131.7078053561803763No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC161261.677942434275559No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT157981.6438133806700534No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT154371.606250611305457No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT137501.4307148996210428No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG120281.2515373681921382No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT112481.1703768138863626No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA111151.156537898857301No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC104001.082140724077007No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT102521.0667410291574495No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA102091.0622667934713619No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC98871.0287620518220544No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT94010.9781927833699944No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT93910.9771522634429972No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT92140.9587350607351481No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA88220.9179466795968609No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG88090.9165940036917647No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT79830.8306470577218025No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT76000.7907951445178127No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC71890.7480297755182309No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC70270.7311733527008776No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT68960.7175425416572153No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC68180.7094264862266377No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG67970.7072413943799437No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT66160.6884079837012959No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT59690.6210863444245821No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT58910.6129702889940045No Hit
GGCTCCTTATCTCCCCTCCCCGTGTCTCCCTTCCCTGATTACCTTTGCGA56390.586749186833677No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT53340.5550133290602648No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT53260.5541809131186671No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC49320.5131844279949805No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA49050.5103750241920884No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG48910.5089182962942923No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT47520.4944550693090324No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA46320.48196883018506687No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA44420.46219895157212154No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC40970.4263010140907209No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG38830.4040338876529824No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG35420.3685521581423806No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT30240.3146532259239297No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCTTCTG29980.31194787411373714No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG29690.3089303663254455No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA25850.26897440112875604No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGT24580.25575979805589255No Hit
CCCCCTCCTCTGTTGCTGTAGAACCAGGTTGGAAAGCCATAGGATTCCTG22510.23422103556705215No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTAGCAGAGACTGGGTCGTAG22100.2299549038663639No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT20740.2158038328592031No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGGGTTGGAAAGCCATA20380.21205796112201344No Hit
GGCCTTTTCCTTCAAACAGGATTTAGCATCTTGTTCTGTTTGTGGATGTA20350.21174580514391433No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA18150.18885436674997763No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC18030.18760574283758108No Hit
TCATTACACCAGTTCGTCCCTTTTCAGCTTTACAGTGAATTGCTGCAACA17690.18406797508579087No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACATATAGCGCCTCTG17650.183651767114992No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC16480.1714776839691257No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA16460.17126957998372627No Hit
GCTGACCTAAAGCCACCTCCCCATGCCCTGAAACAGAGAAGACCTGTAGA16080.1673156042611372No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTTACCGTAGCTCC15140.15753471694736426No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC15090.1570144569838657No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGTAAATGCTATC15010.156182041042268No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA14790.15389289720287433No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT14580.15170780535618036No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA14500.1508753894145827No Hit
ATCTCCGCAAGAAAGGGGAGACTGCCCAACAACACCAGTGTAGAACCATG14080.14650520572119477No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCATACCCC13690.142447178005906No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT11960.12444618326885577No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG11820.1229894553710598No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAACGCCTCTG11710.12184488345136299No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACGGAGATTTCA11170.11622607584557852No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA10900.1134166720426863No Hit
CTTGGGCCTGTGTTATCTCCGTCTTCCCTGCTTGCCTCTGACCCCTGTAG10690.11123158019599234No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA9980.10384388871431277No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT9840.10238716081651679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)