FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940968
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT431684.587616156978771No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA386824.110873058382432No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC268982.858545667865432No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA267772.845686569575161No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC254932.7092313447428604No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA226412.4061392098349783No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG204712.175525628926807No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT186871.9859336342999974No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT183471.9498006308397309No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG178721.8993206995349472No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG171601.8236539393475655No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC169151.7976169221482559No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC166271.7670101427466185No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG164241.7454366142100477No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA161531.7166364849814233No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG161291.7140859200312868No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA159201.6918747502571818No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA154271.6394818952397958No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA150301.5972913000229552No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT147351.5659406058441947No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT136281.4482957975191506No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC136251.4479769769003834No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT132391.406955390619022No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG117541.249139184329329No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC116261.2355361712619346No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC114361.2153441987400209No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG113641.2076925038896116No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT104981.115659618605521No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG102441.0886661395499102No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG101821.082077180095391No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA101431.0779325120514194No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT81180.8627285943836559No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC81140.8623035002252999No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT79610.84604364866818No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGTGCCT79590.845831101589002No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC66580.7075692265836883No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA66410.705762576410675No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC65470.6957728636893072No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC64580.6863145186658844No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT60930.6475246767158925No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG55020.5847170148187824No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA50780.5396570340330383No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAACGTATCATCAT48080.5109631783440033No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG45450.4830132374320912No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT38420.4083029391010109No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA35260.3747205005908809No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG30680.3260472194591102No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG29390.31233793285212674No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC27150.28853265998418653No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG26860.28545072733610494No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG23110.24559814999022284No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGATCA21960.23337669293748567No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA21790.23157004276447235No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTAGGTCTTCAGTGAAC20530.21817957677625596No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA20400.21679802076159868No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTAGAAGGCTG20340.21616037952406458No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGAGGGTGGGAGGCTG19490.20712712865899796No Hit
GACCCAAAACCCAAAATGGCACGGTAGGAGACAGAAGCAGGGAAGACCAA19480.20702085511940896No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC18680.19851897195228746No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT18190.1933115685124255No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC17120.18194029977640047No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG15700.1668494571547598No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGAACGACGTGGTGGAT15260.16217342141284294No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGAAT13900.14772022002873636No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC13810.1467637581724352No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTTAACTTCTGTAACACC13550.1440006461431207No Hit
TCAAATCATCCATTGCTTGGGGTAGCAGAAAGTCAGTCCCATGGAGACCA13440.14283163720764147No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTATCTGTTAAAGAATCATC13080.1390057897824368No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCATAGCAAGGTCTTC12880.13688031899065642No Hit
CCCCTGCTCCTATAGCCAACACTGGAGATGCCGACGAGTACCTCATAGAC12630.13422348050093094No Hit
TCAAATCATCCATTGCTTGGTACGTAGCAGAAAGTCAGTCCCATGGAGAC12440.1322042832487396No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCTAAGCTGTGTCTACTCATT12080.12837843582353492No Hit
TCCACAAACAGAACAAGATGCTGCCGTAATCCTGTTTGAAGGAAAAGGCC11550.1227459382253169No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTATTTTCATTG11480.12200202344819377No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAAGACCA11320.12030164681476947No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGACAACTA11230.11934518495846831No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCTCTCAGGGCAACTG10910.11594443169161969No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGTCAGCCCCTCAGGGCAACTG10110.10744254852449817No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA9440.10032222137203389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)