FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940968
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC341913.6335985920881475No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA286013.039529505785531No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA265962.8264510589095484No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA248582.6417476471038337No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA214232.2766980386155535No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT213482.2687275231463766No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC209932.231000416592275No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA194812.0703148247336784No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG181961.9337533263617892No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC181151.9251451696550785No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG169081.7968730073711328No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG160571.7064342251808775No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT160561.7063279516412886No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT158611.6856046114214298No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC152261.6181209137824029No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA144321.5337397233487218No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA138941.4765645590498295No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT133191.4154572737861435No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT132761.4108875115838158No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA118991.2645488475697366No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC118301.2572159733380943No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG116691.2401059334642623No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC116281.2357487183411124No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT114781.2198076874027597No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT106041.126924613801957No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT102561.0899414220249786No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG100091.0636918577464909No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT99851.0611412927963544No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT99711.0596534632421082No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC99541.0578468130690948No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT98061.0421183292099199No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC96641.0270274865882794No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC92000.9777165642189745No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA90110.95763086523665No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT84520.8982239566063883No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT82570.8775006163865297No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA75660.8040656005305175No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC72460.7700580678620315No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG67320.7154334685132757No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA66660.7084194149004005No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA66510.7068253118065652No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG66280.7043810203960177No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT60780.6459305736220573No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT51460.5468836347250916No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT51220.5443330697749551No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG49370.5246724649509866No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT48780.5184023261152345No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC41510.44114146283401773No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT37840.4021390738048478No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT31720.33709966757636817No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATTAC30280.32179627787554943No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG26940.2863009156528171No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA26930.2861946421132281No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG25750.27365436444172386No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT23480.2495302709550165No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT23340.24804244140077028No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC21330.22668145994337746No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC21240.2257249980870763No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA20340.21616037952406458No Hit
TCCCTGCTTCTGTCTCCTACCGTGCCATTTTGGGTTTTGGGTCTGTAGAA19290.20500165786721758No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT18380.19533076576461686No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA16710.1775830846532507No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA15780.16769964547147193No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC14370.15271507638942025No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA13850.14718885233079126No Hit
CCATGGGACTGACTTTCTGCTACCCCAAGCAATGGATGATTTGATGTAGA13670.14527592861818892No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTTTG13620.14474456092024385No Hit
ATGAGGTACTCGTCGGCATCTCCAGTGTTGGCTATAGGAGCAGGGGTGTA12580.13369211280298587No Hit
CCATGGGACTGACTTTCTGCTACGTACCAAGCAATGGATGATTTGATGTA12550.1333732921842188No Hit
GTTCAAATGAGTAGACACAGCTTAGGTCTTCTCTGTTTCAGGGCACGGTA12260.13029135953613727No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACATATAGCGCCTCTG12140.12901607706106905No Hit
GGCCTTTTCCTTCAAACAGGATTACGGCAGCATCTTGTTCTGTTTGTGGA11600.123277305923262No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA11540.1226396646857279No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC10580.11243740488518206No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGTTGTGGGTTGATTCCACACCCC10140.10776136914326523No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT10110.10744254852449817No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACTAGGGC10080.10712372790573112No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG9420.10010967429285586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)