FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340143
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA3852511.326118720655725No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAAACAATAATG160354.7141937361639075No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA145104.265852891283959No Hit
ACTTCTACCGTGCCCTGCTACCAAGCTGTGTCTACTCATTTGAACAGACC114433.3641733035811408No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC99832.9349420684829615No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA91902.701804829145389No Hit
TGACCAATGGCTAAGTGAAGATGAACACTGACGACATGTTTTTCCTTTCC86532.5439300529483186No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT82442.4236865083215No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG75822.2290624825441063No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA72682.1367483675983334No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC66791.963585903575849No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA59431.7472063220469038No Hit
AGAAAACGGCATTTTGAGTGTAACGGCATTTTGAGGCATAACTGCACCCT57901.7022252405605878No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA57431.6884075227183861No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA49931.4679120252364448No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT46711.3732459583175312No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG46161.3570762885021888No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG45211.3291468588211428No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGGCTAATGAGTTTGTA41491.2197810920701No Hit
GGGGTCAGAGGCAAGCAGTGTGGAGATAACACAGGCCCAAGAGACCAAGT40591.193321632372267No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGATGCCGACGAGTACCTCATAGACC39571.1633342447147228No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC38831.1415786889631714No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT37361.098361571456711No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGGGGGTTGTTGACTTGTA34741.0213351443363528No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGATACACTGAAT34571.0163372463934288No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGCAGCAACAGAGGA33290.9787060148231772No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT31570.928139047400652No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC28730.8446447523541569No Hit
GGAATCCTATGGCTTTCCAACCCATGGTTCTACAGCAACAGAGGAGGGGG24000.7055855919422125No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG22230.6535486545364744No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG19900.5850480533187512No Hit
ATACGGCCAGGCATTGAAGTCTCTGCTACCGTAGAGCAGTCAGAGGACCA17120.5033177222521116No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGGGAGGTGGCTTTAGGTCAGC15930.4683324366516436No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGAT14590.42893724110153675No Hit
TCATAATGCTTGCTCTGATAGGAAGAGATGGAGTGGGTCCCATCAGAGAC12950.3807222256521522No Hit
GACCCAAAACCCAAAATGGCACATGGTTCTACGGTAGGAGACAGAAGCAG12470.36661051381330795No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA12350.3630825858535969No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC11030.3242753782967752No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG10380.30516576851500693No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTAACGGAAGGCAGTCTGGCTGA9740.2863501527298813No Hit
TCTTAAATGGCTACGACCCAGTGACGACATGGCTACCTGTTAAAGAATCA9490.27900030281381655No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGCTTGCAAA8910.2619486510085464No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA8180.24048708925363746No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC7710.22666937141143575No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGATACACTGAATCT7590.2231414434517247No Hit
ATACGGCCAGGCATTGAAGTACACTGAGGACAGGGGCCAGGAGGAGACCA6560.1928600617975381No Hit
ATACGGCCAGGCATTGAAGTCACACTGACGACAGGGGCCAGGAGGAGACC6400.18815615785125667No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGATACACTGAATCTCG6340.18639219387140113No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC5920.17404444601241242No Hit
GGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTACCGTAGATACACT5540.16287267413999407No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA5490.16140270415678112No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGATACAC5340.1569927942071423No Hit
AGGACAAAATGTTTCACTTTTGGGTGCAGATAATGACAAGGAATATCTAG5240.1540528542407164No Hit
TCCCTGCCAGCGAGATGAGACTTGAGGCCTTTGCATCCCTAGACCAAGTC5060.1487609623011498No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGAT5030.14787898031122204No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGAT4980.1464090103280091No Hit
TGTGGTCTGCCAGCTAAAGGGTTTGTACTTTACTTTCATTGGGAGAAGAC4900.1440570583548684No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC4850.14258708837165546No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTAGAT4830.14199910037837027No Hit
AGCTGCTCACCATCGCTACCGTACAGGTCTTGGCCAGTTGGAGACCAAGT4630.1361192204455185No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT4580.13464925046230555No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG4570.13435525646566296No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG4540.1334732744757352No Hit
AGGACAAAATGTTTCACTTTTGGTAAATACATTCTTCATACCAGGACCAG4530.1331792804790926No Hit
TGAGCAAGAGGCTTTGGAGTTGGTTCTACTCTCAGCAGGCAAAGACCAGA4440.13053333450930932No Hit
AGGACAAAATGTTTCACTTTGGGTAAATACATTCTTCATACCAGGACCAG4340.12759339454288343No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA3880.11406967069732436No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA3730.10965976074768555No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGGAGACCA3700.10877777875775776No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGGA3480.10230991083162082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)