FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340143
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC3461110.175426217796632No Hit
CTCGATAATCTGGATGACTCATTATTGTTTACGGTAGCACAACTAAAAGA152724.489876316725613No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA145204.268792831250385No Hit
GTTCAAATGAGTAGACACAGCTTGGTAGCAGGGCACGGTAGAAGTTGTAG114643.3703471775106353No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA100452.953169696274802No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA91992.7044507751151725No Hit
GTTCAAATGAGTAGACACAGCTTGGTCTTCTCTGTTTCAGGGCACGGTAG82852.435740262183846No Hit
TCCAGGAAGAGGAAAGGAAAAACATGTCGTCAGTGTTCATCTTCACTTAG79302.3313723933757275No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT72372.1276345537024137No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG71622.105585003954219No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA66721.961527945599351No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT59411.7466183340536185No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT58601.722804820325569No Hit
AAGGGTGCAGTTATGCCTCAAAATGCCGTTACACTCAAAATGCCGTTTTC56361.656950165077629No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG50451.4831997130618593No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT49841.4652660792666614No Hit
AACAGAGAATCTCCATTTTAGCACCAGAGACTTTGTCATATTTTTCCCCT45801.3464925046230556No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT44881.3194450569319374No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGACCTAATTACG41341.2153711821204611No Hit
CTTGGGCCTGTGTTATCTCCACACTGCTTGCCTCTGACCCCTGTAGAACC40851.2009654762849744No Hit
ATGAGGTACTCGTCGGCATCAGAGACTTGGCTATAGGAGCAGGGGTGTAG39611.1645102207012932No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG38711.1380507610034603No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT38421.1295249351008252No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG35311.0380928021449802No Hit
ACAAGTCAACAACCCCCACACTGACTTCCATTTTCTACTTTTTCTGAGGT34861.0248630722960637No Hit
CCCCCTCCTCTGTTGCTGCCATGTCGTCAGTGTGGTTGGAAAGCCATAGG32910.967534242950759No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT32820.9648882969809756No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT31860.9366648733032872No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG29200.8584624701963586No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGGGTTGGAAAGCCATAGGATTCC24180.7108774838817791No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC22560.6632504564256797No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG20110.5912219272482455No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC18860.554472677667922No Hit
CCTGGTCCTCTGACTGCTCTACGGTAGCAGAGACTTCAATGCCTGGCCGT17680.5197813860640965No Hit
GCTGACCTAAAGCCACCTCCCTTCTACCATGCCCTGAAACAGAGAAGACC16070.4724483526046398No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA15620.45921862275572334No Hit
CTGATGGGACCCACTCCATCTCTTCCTATCAGAGCAAGCATTATGATGTA13020.3827801836286503No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCAGCATCTTGTTCTGTTTG12150.3572027059207451No Hit
TCCCTGCTTCTGTCTCCTACCGTAGAACCATGTGCCATTTTGGGTTTTGG11780.34632492804496934No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA10500.308693696474718No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA10470.3078117144847902No Hit
CAGCCAGACTGCCTTCCGTTACGGTAGCAGAGACCTGTGGGAAGCGAAAT9770.287232134719809No Hit
TCCAGATGATTCTTTAACAGGTAGCCATGTCGTCACTGGGTCGTAGCCAT8570.2519528551226984No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATGTCGTCAGTGTACTATCAA8240.24225105323349297No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA7850.23078528736443202No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC7800.22931531738121907No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG7690.2260813834181506No Hit
CCTCCTGGCCCCTGTCCTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA6580.19344804979082328No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG6520.19168408581096774No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC6460.1899201218311122Illumina Single End PCR Primer 1 (100% over 21bp)
CCTCCTGGCCCCTGTCGTCAGTGTGACTTCAATGCCTGGCCGTATTGTAG6420.18874414584454186No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG5920.17404444601241242No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC5570.16375465612992182No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT5560.16346066213327923No Hit
TCATTACACCAGTTCGTCCCTGTAGAACCATGTCGTCAGTGTAGATCTCG5560.16346066213327923No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC5460.16052072216685337No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA5150.1514069082709331No Hit
AGGGATGCAAAGGCCTCAAGTCTCATCTCGCTGGCAGGGATGTAGAACCA5100.14993693828772017No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA5070.1490549562977924No Hit
CCCAGGAAGAGGAAAGGAAAAACATGTCGTCAGTGTTCATCTTCACTTAG4950.14552702833808134No Hit
ACTAGATATTCCTTGTCATTATCTGCACCCAAAAGTGAAACATTTTGTCC4940.14523303434143875No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTAGCTGGCAGACCACATGTA4820.14170510638172767No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA4790.14082312439179992No Hit
CCAACTGGCCAAGACCTGTACGGTAGCGATGGTGAGCAGCTTGTAGAACC4650.13670720843880366No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA4570.13435525646566296No Hit
GGTCTTTGCCTGCTGAGAGTAGAACCAACTCCAAAGCCTCTTGCTCATGT4500.13229729848916486No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC4480.13170931049587967No Hit
CCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG4310.12671141255295568No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT4190.1231834845932446No Hit
CCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA3730.10965976074768555No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTAGCAGAGACTGGGTCGTAG3730.10965976074768555No Hit
CCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT3480.10230991083162082No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA3430.10083994084840787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)