FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134481
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA83526.210542753251389No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGAACAATAATGAG61554.576854722972018No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA51373.8198704649727473No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA42283.143938548939999No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG33472.488827417999569No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA26891.9995389683300988No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT26161.9452562071965558No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAAGTCATCGCAAT26151.944512607728973No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG25631.9058454354146683No Hit
ACTTCTACCGTGCCCTGAAGCTGTGTCTACTCATTTGAACAGACCAAGTC25341.8842810508547674No Hit
ACTTCTACCGTGCCCTGCTACCGTAAGCTGTGTCTACTCATTTGAACAGA24991.85825506948937No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC22431.6678936057881781No Hit
TCACAATTGCCAGTTAACGTCTACACTGACGACATGTTTCTGCTTTGCTG22391.6649192079178472No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA21231.5786616696782445No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA19861.4767885426194034No Hit
TGACAGTTTGACAGTTAAAGGCATATTAAACCTCAGCCGTTACCTGTGTG19681.4634037522029135No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG19241.430685375629271No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG18851.4016849963935425No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA18001.3384790416490062No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC17811.3243506517649333No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT17451.2975810709319533No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA17171.2767602858396354No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG15961.186784750262119No Hit
GACCCAAAACCCAAAATGGCCACGGTAGGAGACAGAAGCAGGGAAGACCA15641.1629895672994697No Hit
GGGGTCAGAGGCAAGCAGCTGGGGAGGCTGAGGCAGGAGATAACACAGGC12570.934704530751556No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG12380.9205761408674832No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT12180.9057041515158275No Hit
ACTTCTACCGTGCCCTGAGCTGTGTCTACTCATTTGAACAGACCAAGTCT11780.8759601728125164No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC11080.8239082100817215No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGGCTAATGAGTTTGTA9580.7123682899443043No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTGAGAACGTGGGAGTAGACGGAA8760.6513931336025163No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC8550.6357775447832779No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG7960.5919051761958939No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA7870.5852127809876488No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG7390.5495200065436753No Hit
TCATAATGCTTGCTCTGATAGGAGCAGGGGATGGAGTGGGTCCCATCAGA7230.5376224150623509No Hit
GGAATCCTATGGCTTTCCAACCGTTCTACAGCAACAGAGGAGGGGGAGAC6920.5145708315672846No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAAGT5400.40154371249470183No Hit
GGGGTCAGAGGCAAGCAGTCAAAGCACATGGGAGATAACACAGGCCCAAG5180.3851845242078807No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAAGTCATCGCAATCTCG5130.38146652686996674No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT5010.3725433332589734No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA4910.3651073385831456No Hit
TCCCTGCCAGCGAGATATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGC4230.31454257478751646No Hit
TCTGTATCAGGCAAAGTCATAGAACCATGTCGGGAGCCATTGTCTTTGAG4200.31231177638476815No Hit
GGGGTCAGAGGCAAGCAGTCACAGGGAGATAACACAGGCCCAAGAGACCA4070.30264498330619194No Hit
ACAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAAGGA4040.3004141849034436No Hit
GGAATCCTATGGCTTTCCAACCCATGGTTCTACAGCAACAGAGGAGGGGG4020.29892698596827805No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT4010.29818338650069526No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTGTGGGGGTTGTTGACTT3870.2877729939545363No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAA3790.2818241982138741No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAAGTCATCGCAATCT3710.27587540247321185No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA3460.2572854157836423No Hit
TGAGCAAGAGGCTTTGGAGTAACTCTCAGCAGGCAAAGACCAGACCAAGT3450.2565418163160595No Hit
TCCCTGCCAGCGAGATAGACTGAGGCCTTTGCATCCCTAGACCAAGTCTC3440.2557982168484767No Hit
TCCCTGCCAGCGAGATGGGATGAGGCCTTTGCATCCCTAGACCAAGTCTC3380.25133662004298No Hit
ATACGGCCAGGCATTGAAGTACATTGACGACAGGGGCCAGGAGGAGACCA3350.2491058216402317No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG3300.24538782430231781No Hit
GGAATCCTATGGCTTTCCAACCCTGACGACATGGTTCTACAGCAACAGAG3290.24464422483473502No Hit
GTAGAGGAGCCGTCAAATCCAACATGGTTCTACAGTAGAGGAGCCGTCAA3260.24241342643198668No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAAGT3110.23125943441824498No Hit
CAGTCAGAGGCGCTATGTGTATTACTATAGCTACCTGTTAAAGAATCATC2920.2171310445341721No Hit
GGAATCCTATGGCTTTCCAACCCTGACGACGGTTCTACAGCAACAGAGGA2600.19333586157152313No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGCTAATG2570.19110506316877476No Hit
GGTCTTCTCTGTTTCAGGGCATGTCGGGGAGGTGGCTTTAGGTCAGCAGA2470.18366906849294698No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2440.1814382700901986No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT2290.17028427807645688No Hit
GGTCTTCTCTGTTTCAGGGCATCAGTGGGAGGTGGCTTTAGGTCAGCAGA2230.16582268127096023No Hit
GGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAAGTCATCG2170.16136108446546352No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAAGT2160.16061748499788073No Hit
TGTGGTCTGCCAGCTAAAGGTTTGTACTTTACTTTCATTGGGAGAAGACC2150.15987388553029797No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT2030.15095069191930457No Hit
AGCTGCTCACCATCGCTATACGGAGCAGGTCTTGGCCAGTTGGAGACCAA1880.13979669990556287No Hit
GGAATCCTATGGCTTTCCAACCACGACATGGTTCTGCAGCAACAGAGGAG1870.1390531004379801No Hit
ACTTCTACCGTGCCCTAAGCTGTGTCTACTCATTTGAACAGACCAAGTCT1840.13682230203523174No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAAGTCATC1810.1345915036324834No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC1720.12789910842423835No Hit
GGAAACCGTAGCTGCCCTGCTACCGTATTCATCTGGACCTGGGTCTTAGA1680.12492471055390725No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC1640.12195031268357612No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAGAGACCAAG1550.1152579174753311No Hit
TGTGCTGTGACTGCTTGTAGAACCATGTACAGAGTTGAAAGTCAGGGCAA1490.1107963206698344No Hit
GGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTACCGTAAGTCATCG1350.10038592812367546No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAAGTCATCG1350.10038592812367546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)