FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134481
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA79095.881128189112217No Hit
CTCGATAATCTGGATGACTCATTATTGTTCGGTAGCACAACTAAAAGAAA59334.41177564116864No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA50003.7179973379139057No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA42483.1588105382916543No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC33122.4628014366341713No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG26962.0047441646031783No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT26922.0017697667328473No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT26731.9876413768487742No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC26611.9787181832377807No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT25691.910307032220165No Hit
GTTCAAATGAGTAGACACAGCTTCAGGGCACGGTAGAAGTTGTAGAACCA25431.8909734460630125No Hit
GTTCAAATGAGTAGACACAGCTTACGGTAGCAGGGCACGGTAGAAGTTGT24951.855280671619039No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA22351.661944810047516No Hit
CCACACAGCAAAGCAGAAACATGTCGTCAGTGTAGACGTTAACTGGCAAT22281.6567396137744366No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT21301.583866865951324No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG21151.5727128739375822No Hit
CACACACAGGTAACGGCTGAGGTTTAATATGCCTTTAACTGTCAAACTGT19571.455224158059503No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG18481.3741718160929797No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT18311.3615306251440724No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA18251.3570690283385758No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG17791.3228634528297678No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA17241.2819654821127149No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA16081.1957079438731122No Hit
TCCCTGCTTCTGTCTCCTACCGTGGCCATTTTGGGTTTTGGGTCTGTAGA15651.1637331667670525No Hit
CTTGGGCCTGTGTTATCTCCTGCCTCAGCCTCCCCAGCTGCTTGCCTCTG12570.934704530751556No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT12480.9280121355433109No Hit
GTTCAAATGAGTAGACACAGCTCAGGGCACGGTAGAAGTTGTAGAACCAT11720.8714985760070195No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT11240.8358058015630461No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG9750.7250094808932117No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGACCTAATTACG9210.6848551096437415No Hit
TCCGTCTACTCCCACGTTCTCACGGTAGCAGAGAGATGGCAGAAGCTGCT8740.6499059346673508No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA8250.6134695607557945No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC7870.5852127809876488No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA7510.5584432001546686No Hit
CTGATGGGACCCACTCCATCCCCTGCTCCTATCAGAGCAAGCATTATGAT7220.536878815594768No Hit
CCCCCTCCTCTGTTGCTGTAGAACGGTTGGAAAGCCATAGGATTCCTGTA6980.5190324283727813No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG6890.5123400331645362No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA5440.404518110365033No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC5380.4000565135595363Illumina Single End PCR Primer 1 (100% over 21bp)
CTTGGGCCTGTGTTATCTCCCATGTGCTTTGACTGCTTGCCTCTGACCCC5230.3889025215457946No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTAGCAGAGACTGGGTCGTAG4850.36064574177764885No Hit
AGGGATGCAAAGGCCTCATATCTCGCTGGCAGGGATGTAGAACCATGTCG4530.33685055881499987No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT4520.33610695934741713No Hit
GCCTCAAAGACAATGGCTCCCGACATGGTTCTATGACTTTGCCTGATACA4210.3130553758523509No Hit
CCTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA4120.30636298064410583No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT4100.3048757817089403No Hit
CTTGGGCCTGTGTTATCTCCCTGTGACTGCTTGCCTCTGACCCCTGTAGA4090.3041321822413575No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGGGTTGGAAAGCCATAGGATTCC4000.2974397870331125No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA3930.292234590760033No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC3860.2870293944869535No Hit
AGGGATGCAAAGGCCTCAGTCTATCTCGCTGGCAGGGATGTAGAACCATG3730.27736260140837743No Hit
ACAAGTCAACAACCCCCACAGCAGAGACTTCCATTTTCTACTTTTTCTGA3710.27587540247321185No Hit
AGGGATGCAAAGGCCTCATCCCATCTCGCTGGCAGGGATGTAGAACCATG3650.2714138056677151No Hit
GGTCTTTGCCTGCTGAGAGTTACTCCAAAGCCTCTTGCTCATGTAGAACC3490.25951621418639065No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT3450.2565418163160595No Hit
CCTCCTGGCCCCTGTCGTCAATGTACTTCAATGCCTGGCCGTATTGTAGA3430.2550546173808939No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG3380.25133662004298No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA3330.24761862270506613No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGGGTTGGAAAGCCATA3190.2372082301589072No Hit
TTCATGGTGTTTTATCCCTCTTGACGGCTCCTCTACTGTAGAACCATGTT3070.22828503654791382No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG2640.19631025944185426No Hit
CCCCCTCCTCTGTTGCTGTAGAACCGTCGTCAGGGTTGGAAAGCCATAGG2580.19184866263635755No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC2560.19036146370119197No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAGTAATACACATAGCGCCTCTG2490.18515626742811253No Hit
GCTGACCTAAAGCCACCTCCCCGACATGCCCTGAAACAGAGAAGACCTGT2480.18441266796052974No Hit
GCTGACCTAAAGCCACCTCCCACTGATGCCCTGAAACAGAGAAGACCTGT2280.16954067860887412No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC2270.16879707914129133No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTAGCTGGCAGACCACATGTAG2220.16507908180337744No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT2170.16136108446546352No Hit
GCCTCAAAGACAATGGCTCCTGTAGAACCATGTCGTCAGTGTAGATCTCG2140.15913028606271518No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA2110.15689948765996684No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT2070.1539250897896357No Hit
CCAACTGGCCAAGACCTGCTCCGTATAGCGATGGTGAGCAGCTTGTAGAA2030.15095069191930457No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC1840.13682230203523174No Hit
GTTCAAATGAGTAGACACAGCTTAGGGCACGGTAGAAGTTGTAGAACCAT1830.13607870256764895No Hit
CCCCCTCCTCTGTTGCTGCAGAACCATGTCGTGGTTGGAAAGCCATAGGA1790.13310430469731785No Hit
AAGACCCAGGTCCAGATGAATACGGTAGCAGGGCAGCTACGGTTTCCTGT1700.1264119094890728No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA1580.11748871587807944No Hit
TGCCCTGACTTTCAACTCTGTACATGGTTCTACAAGCAGTCACAGCACAT1490.1107963206698344No Hit
CTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC1480.11005272120225161No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC1410.10484752492917215No Hit
TCATTACACCAGTTCGTCCCTGTAGAACCATGTCGTCAGTGTAGATCTCG1390.10336032599400659No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG1380.1026167265264238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)