FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909651
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT484025.320941767776873No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA361713.97636016450265No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA271382.9833419630165854No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT239222.6297997803553232No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG234452.577362087218065No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC220252.4212582627842987No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA210702.3162729442390546No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT162951.791346351512833No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG162531.7867291961422567No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG160501.764412945184472No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC143221.57444998136648No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA140671.546417252330839No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG139111.5292678180972703No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT138371.521132830063398No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC137781.5146468260904458No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA137241.5087104834711333No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC130521.4348359975419145No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT128961.4176865633083457No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT123031.3524967267666392No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC121971.3408439060694706No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA118931.3074244957681571No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA114791.2619125356867635No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT109501.203758364471649No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGATGGC102181.1232879422987496No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC101831.1194403128232695No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC99131.0897585997267085No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT97681.0738184204711476No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCAGATAAGATGCTGAGG95411.0488637950158908No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG92151.0130258747585612No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA89080.9792766676450638No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG85530.9402507115366223No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG79610.8751708072656437No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG79320.8719827714145315No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT73770.8109703611604892No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC69780.7671073851400152No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG69600.7651286042669112No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG63890.7023572776812206No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC57380.6307913694372896No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG56650.6227663136741454No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC55600.6112234252477049No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT54640.6006699272578164No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT52870.5812119153389597No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC31350.34463766873229407No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA31190.34287875240064597No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAACATAGTATCAT30590.3362828161569657No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG30360.3337543739302216No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA30120.33111599943274944No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATTATC30020.33001667672546947No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT26350.28967153336829177No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGATCA25100.27592999952729125No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA24760.2721923023225391No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAAAGATGGAGGTGAGAGGCTG24420.2684546051177869No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG23150.2544932067353304No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC22960.25240449359149825No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA22710.24965618682329815No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC20260.22272278049493707No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG20030.22019433826819296No Hit
ACTTCTACCGTGCCCTGATGGATGAAAAAGACATGGACGACGTGGTGGAT18770.2063428721564644No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG18530.20370449765899232No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA17340.19062255744235976No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAAGCTG17020.18710472477906362No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG14360.15786274076541443No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTAGGAGGCTG13860.1523661272290142No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTTTCTAGGTG13850.15225619495828618No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA13430.14763903958771002No Hit
TGACCAATGGCTAAGTGAAGATGTTTTTCCTTTCCTCTTCCTGGAAGACC13320.14642978460970196No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTTCTCCAATTCAGGACCCA12890.14170269696839777No Hit
ACAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAAGGA12890.14170269696839777No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATT12280.1349968284539895No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACAACGTGGTGGAT12150.13356770893452544No Hit
GACCCAAAACCCAAAATGGCAAAGGAGGGAGAGATGGGGGTGGAAGGCTG12130.13334784439306943No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACTA11660.1281810276688532No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA11600.1275214340444852No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGTAGCTCGTGGTG11490.12631217906647713No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTTATACCAGGACCA10300.11323023884984461No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTAGAT10270.1129004420376606No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG9790.10762369304271639No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGAAAGCTG9780.10751376077198839No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCTTCACAGCAGGGTCTTC9540.10487538627451627No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC9220.10135755361122013No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA9220.10135755361122013No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG9170.1008078922575801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)