FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141994
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAATACCCTCCATGTGCAGATAATGA88416.226319421947406No Hit
ACTTCTACCGTGCCCTGACCCCAAGCTGTGTCTACTCATTTGAACAGACC66334.671324140456639No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA60754.278349789427723No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGTCTCTGCTACCGTAGGGACG55383.9001647956955923No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC43263.0466076031381606No Hit
CAGTCAGAGGCGCTATGTGTAGAATGGAACAGTTTTCTTAACCATATCTA40752.8698395706860853No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA34232.4106652393763115No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA32362.2789695339239686No Hit
ACTTCTACCGTGCCCTGAAGCTGTGTCTACTCATTTGAACAGACCAAGTC30682.160654675549671No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC29862.102905756581264No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA29602.0845951237376226No Hit
AGGACAAAATGTTTCACTTTTGGGTACGGTGCAGATAATGACAAGGAATA29422.071918531768948No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG28572.012056847472428No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG27291.9219121934729637No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG27201.9155738974886265No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA24111.6979590686930435No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAAGCATCTATAAT23461.65218248658394No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT22691.5979548431623871No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC21071.4838655154443146No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG19411.3669591672887587No Hit
CAGCCTCTGGCATTCTGCTACCGTAGAGCAGTCAGAGGACCAGGAGACCA17051.2007549614772455No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC15651.102159246165331No Hit
CAGAGGGGAAAAATATGACAAAGTGCTAAAATGGAGATTCTCTGTTAGAC13600.9577869487443132No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTATGCTACCTGTTAAAG12350.8697550600729609No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC11940.8408806005887572No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC11490.8091891206670705No Hit
TACCCTCCATGAGGCACACCAAGTCTCTACTACCGTAAGCCAGAATTTAC9900.6972125582771103No Hit
AGAAAACGGCATTTTGAGTGTGTGTGTGTGTGTGTGTGTGAGGCATAACT7950.5598828119498007No Hit
AGAAAACGGCATTTTGAGTGTGTGTGTGTGTGTGTGTGAGGCATAACTGC7830.5514317506373508No Hit
GGGGTCAGAGGCAAGCAGACCCCGGGGGAGATAACACAGGCCCAAGAGAC7640.5380509035593053No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC7520.5295998422468554No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAAGC6800.4788934743721566No Hit
GGAATCCTATGGCTTTCCAACCCTGACGACATGGTTCTACAGCAACAGAG6620.4662168824034818No Hit
TCACAATTGCCAGTTAACGTTTCTGCTTTGCTGTGTGGAGACCAAGTCTC6530.4598785864191444No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG6480.45635731087229037No Hit
TCAAATCATCCATTGCTTGGTACGGGTAGCAGAAAGTCAGTCCCATGGAG6200.43663816780990744No Hit
TCTTAAATGGCTACGACCCAGTCTCTACTACCTGTTAAAGAATCATCTGG6090.4288913616068284No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC6070.4274828513880868No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT5840.411284983872558No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAAGCATCTATAATCT5810.4091722185444456No Hit
TGAGCAAGAGGCTTTGGAGTAACCAACCCAACTCTCAGCAGGCAAAGACC4900.3450850035917011No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAAGC4840.34085947293547614No Hit
ATACGGCCAGGCATTGAAGTACTCTGACGACAGGGGCCAGGAGGAGACCA4730.33311266673239714No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAA4680.3295913911855431No Hit
AGAAAACGGCATTTTGAGTGAGGCATAACTGCACCCTTAGACCAAGTCTC4490.3162105441074975No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT3930.27677225798273164No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG3760.2647999211234277No Hit
TCACAATTGCCAGTTAACGTCTCTGCTTTGCTGTGTGGAGACCAAGTCTC3730.2626871557953153No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAAGCATCTATAATCTCG3630.2556446047016071No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCCGTACGGAAGGCAGTCTGGCTGA3570.25141907404538216No Hit
GCGTGGACAACCCCCACCTGACGACATGGTTCTACAGCAACAGAGGAGGG3560.2507148189360114No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA3480.24508077806104483No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA3300.2324041860923701No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTAAGC3260.22958716565488682No Hit
GGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAAGCATCTA3000.21127653281124556No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT2970.2091637674831331No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT2960.20845951237376228No Hit
GGGGTCAGAGGCAAGCAGGCCCAAGAGACCAAGTCTCTGCTACCGTAAGC2950.20775525726439142No Hit
AGAAAACGGCATTTTGAGTGTGTGTGTGTGTGTGTGAGGCATAACTGCAC2880.2028254714987957No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA2540.1788807977801879No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG2210.1556403791709509No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC2200.15493612406158008No Hit
ACAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAAGGA2130.15000633829598434No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2080.14648506274913023No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC2050.1443722974210178No Hit
GCGTGGACAACCCCCACAGCCGGAGGGGAGATAAGGAGCCAGACCAAGTC2030.14296378720227615No Hit
AGCTGCTCACCATCGCTACCCTAAGGTCTTGGCCAGTTGGAGACCAAGTC2000.1408510218741637No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAAGC1990.14014676676479287No Hit
AGCTGCTCACCATCGCTACCGCAGGTCTTGGCCAGTTGGAGACCAAGTCT1840.1295829401242306No Hit
GGGGTCAGAGGCAAGCAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGGA1840.1295829401242306No Hit
GAGAAAGCCCCCCTACTGCACACTGACTTCCACTCGGATAAGATGCTAGA1600.11268081749933095No Hit
TGACCAATGGCTAAGTGAAGATGAGTCATATTTGTTTTTCCTTTCCTCTT1540.10845528684310604No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGGAGACCA1510.10634252151499358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)