FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141994
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACATGGAGGGTATTACCCAAAAGT84775.969970562136428No Hit
GTTCAAATGAGTAGACACAGCTTGGGGTCAGGGCACGGTAGAAGTTGTAG66404.676253926222235No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA60594.267081707677789No Hit
TCATTACACCAGTTCGTCCCTACGGTAGCAGAGACGTGGTAAAAAGATAA52683.7100159161654718No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA43613.0712565319661396No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT39282.7663140696085753No Hit
TCTAGATATGGTTAAGAAAACTGTTCCATTCTACACATAGCGCCTCTGAC39042.7494119469836753No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA34342.4184120455793905No Hit
GTTCAAATGAGTAGACACAGCTTCAGGGCACGGTAGAAGTTGTAGAACCA30682.160654675549671No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT29922.1071312872374888No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA29792.0979759708156682No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTACCCAAAAGTGAAACATTT28111.9796611124413708No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT27411.9303632547854135No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG27131.9106441117230306No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT26621.8747271011451188No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT24321.7127484259898307No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG23861.6803526909587732No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG23291.6402101497246362No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT20671.455695311069482No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG18281.2873783399298562No Hit
CCTGGTCCTCTGACTGCTCTACGGTAGCAGAATGCCAGAGGCTGTGTAGA17011.1979379410397621No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA15731.1077932870402973No Hit
AACAGAGAATCTCCATTTTAGCACTTTGTCATATTTTTCCCCTCTGTGTA13640.9606039691817965No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG12050.8486274067918362No Hit
TCCAGATGATTCTTTAACAGGTAGCATACGGTAGCAGAGACTGGGTCGTA11540.8127103962139245No Hit
GGAGAGCTGTAAATTCTGGCTTACGGTAGTAGAGACTTGGTGTGCCTCAT10010.7049593644801894No Hit
AAGGGTGCAGTTATGCCTCACACACACACACACACACACTCAAAATGCCG7840.5521360057467217No Hit
AAGGGTGCAGTTATGCCTCACACACACACACACACACACACTCAAAATGC7790.5486147301998676No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA7760.5465019648717552No Hit
CTTGGGCCTGTGTTATCTCCCCCGGGGTCTGCTTGCCTCTGACCCCTGTA7400.5211487809344056No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA6850.4824147499190107No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC6840.48171049480963984No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGGGTTGGAAAGCCATA6760.4760764539346733No Hit
CCACACAGCAAAGCAGAAACGTTAACTGGCAATTGTGATGTAGAACCATG6560.4619913517472569No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG6560.4619913517472569No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC6560.4619913517472569No Hit
CCATGGGACTGACTTTCTGCTACCCGTACCAAGCAATGGATGATTTGATG6210.4373424229192783No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC6080.42818710649745767No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT5810.4091722185444456No Hit
TCCAGATGATTCTTTAACAGGTAGTAGAGACTGGGTCGTAGCCATTTAAG5750.40494668788822064No Hit
GGTCTTTGCCTGCTGAGAGTTGGGTTGGTTACTCCAAAGCCTCTTGCTCA4930.3471977689198135No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA4920.3464935138104427No Hit
CCTCCTGGCCCCTGTCGTCAGAGTACTTCAATGCCTGGCCGTATTGTAGA4780.33663394227925125No Hit
CTTGGGCCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTGTAGA4730.33311266673239714No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA4670.3288871360761722No Hit
AAGGGTGCAGTTATGCCTCACTCAAAATGCCGTTTTCTTGTAGAACCATG4460.31409777877938505No Hit
CCTAGATATTCCTTGTCATTATCTGCACATGGAGGGTATTACCCAAAAGT4430.3119850134512726No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT4060.2859275744045523No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA3820.26902545177965265No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC3750.26409566601405693Illumina Single End PCR Primer 1 (100% over 21bp)
CCACACAGCAAAGCAGAGACGTTAACTGGCAATTGTGATGTAGAACCATG3730.2626871557953153No Hit
CAGCCAGACTGCCTTCCGTACGGGTAGCAGAGACCTGTGGGAAGCGAAAT3620.2549403495922363No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGGTGG3460.24367226784230317No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG3380.23803822696733665No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA3340.23522120652985337No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTAGCAGAGACTGGGTCGTAG3290.2316999309829993No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA3110.21902333901432455No Hit
GCCTCAAAGACAATGGCTCCTGTAGAACCATGTCGTCAGTGTAGATCTCG3050.21479780835809964No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT3000.21127653281124556No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT2970.2091637674831331No Hit
AAGGGTGCAGTTATGCCTCACACACACACACACACACTCAAAATGCCGTT2720.19155738974886263No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC2460.17324675690522134No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC2370.166908460920884No Hit
CCATTACACCAGTTCGTCCCTACGGTAGCAGAGACGTGGTAAAAAGATAA2220.1563446342803217No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG2170.1528233587334676No Hit
GGCTCCTTATCTCCCCTCCGGCTGTGGGGGTTGTCCACGCTGTAGAACCA2080.14648506274913023No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA2030.14296378720227615No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG2020.14225953209290534No Hit
CCAACTGGCCAAGACCTTAGGGTAGCGATGGTGAGCAGCTTGTAGAACCA1970.13873825654605124No Hit
CCAACTGGCCAAGACCTGCGGTAGCGATGGTGAGCAGCTTGTAGAACCAT1890.13310421567108469No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC1770.12465315435863486No Hit
AGCATCTTATCCGAGTGGAAGTCAGTGTGCAGTAGGGGGGCTTTCTCTGT1660.11690634815555585No Hit
CCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA1550.10915954195247686No Hit
CCTAGATATGGTTAAGAAAACTGTTCCATTCTACACATAGCGCCTCTGAC1510.10634252151499358No Hit
CGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG1450.10211699085876869No Hit
TCCAGGAAGAGGAAAGGAAAAACAAATATGACTCATCTTCACTTAGCCAT1450.10211699085876869No Hit
CCTAGATATTCCTTGTCATTATCTGCACCGTACCCAAAAGTGAAACATTT1430.10070848064002705No Hit
CCAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT1420.10000422553065622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)