FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654437
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT347295.306698734943165No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA320854.902687348056421No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA238303.641297787258361No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC200913.069967009811487No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG191062.9194559598555703No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA178282.7241736026538845No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG158122.4161225603075622No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT153032.338345784238972No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG143042.185695490933428No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC137172.096000073345486No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA132192.0199041313373174No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT130711.9972892730698295No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA107161.637437981043248No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT104881.6025988750636044No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG104651.5990844038463594No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC102271.5627172669026963No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC99001.5127506543792604No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT97241.485857309412518No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC92601.414956672682015No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA92421.4122062169467802No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG84001.283546009776342No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT83911.2821707819087247No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA81221.2410667489766012No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT73741.126770032867946No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG67741.0350881750267789No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT66551.0169046065549474No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG66081.0097228610240558No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATT54090.8265119484381231No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG50700.7747116987578636No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT50290.7684467718053839No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG44790.6844050687843138No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC40840.6240478457055454No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC40210.6144212506322229No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC39380.6017385936308614No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAAGTCAGACGCAT36960.5647602443015906No Hit
CAGTCAGAGGCGCTATGTGTACTATTATAGCTACCTGTTAAAGAATCATC34270.5236562113694672No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA33450.5111263574645076No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT31580.4825521784373439No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC30020.4587148953986404No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA28600.4370168557095641No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTTTTC28210.43105753494988824No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAACAGTTCAACTTCTGTAACACC28090.4292238977930649No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG26580.4061506302363711No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA24760.378340466691217No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT24650.37665963263079566No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTAGGAGGCTG24570.37543720785958007No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC24500.37436758618476645No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA22940.350530303146063No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG21150.3231785488901147No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC21130.3228729426973108No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG20780.31752483432324274No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGAACCA19860.3034669494542638No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA18060.2759623921019136No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA15450.2360807839410058No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG15410.23546957155539802No Hit
TGACCAATGGCTAAGTGAAGATGTTTTTCCTTTCCTCTTCCTGGAAGACC14660.22400933932525208No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGAATAAGATGCTGAGG14240.2175916092763704No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG13860.21178509161309647No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT13290.20307531511818555No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGAGAGGCTG12160.18580856522476571No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCCGTTAAAGAATCATC12100.18489174664635405No Hit
ACAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAAGGA12050.1841277311643443No Hit
ACTTCTACCGTGCCCTGATACAGATGAAGCTGTGTCTACTCATTTGAACA11600.17725159182625677No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG11320.17297310512700229No Hit
AGAAAACGGCATTTTGAGTGTTCAAGGTTTGAGGCATAACTGCACCCTTA11010.16823620913854198No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT10470.1599848419328369No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT10470.1599848419328369No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA10050.15356711188395522No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTTTCTTTAACAATT9960.1521918840163377No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC8580.13110505671286923No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAAGTCAGACGCATCTCG8240.12590975143520308No Hit
GACCCAAAACCCAAAATGGCAGGGGAGAGAGAGATGGGGGTGGGAGGCTG7680.11735277803669414No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT7400.11307429133743967No Hit
ACTTCTACCGTGCCCTGAAGCTGTGTCTACTCATTTGAACAGACCAAGTC7220.11032383560220463No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC7060.10787898605977352No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG6940.10604534890295016No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA6850.10467012103533267No Hit
TCAAATCATCCATTGCTTGGCGGTAGCAGAAAGTCAGTCCCATGGAGACC6680.1020724683964996No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA6680.1020724683964996No Hit
GTAGAGGAGCCGTCAAATCCATGTCGTCATGTCAAGAGGGATAAAACACC6590.10069724052888208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)