FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363235
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC292858.062273734634603No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA239946.6056409762275115No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC105852.914091428414112No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC104932.8887634726829736No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA72892.0066898839594205No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGATCGTCTCTAT71411.9659449116962848No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA69851.9229975085000068No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG69361.9095076190345093No Hit
AGGACAAAATGTTTCACTTTTGGGTAATTACGGTGCAGATAATGACAAGG63991.761669442647322No Hit
AGAAAACGGCATTTTGAGTGTATCTGCATGTGGATATCTGGAGCGATTTG53451.4714991672058035No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG53221.4651671782730187No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC52431.4434181728082371No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT51871.42800115627624No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA51701.4233209905433122No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGGTAGGGACGAACTGGTGTAA48761.3423816537503268No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA43491.1972965160295677No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA42111.1593045824328603No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC40371.111401709637012No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG38271.0535878976420225No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG35250.9704461299158947No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT34310.9445675664514708No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG34150.9401627045851859No Hit
TGACCAATGGCTAAGTGAAGATGAACACTGACGACTTGTTTTTCCTTTCC33730.928599942186188No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG30020.8264622076617066No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG28870.7948022629977838No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGCTACCTATGGATGCCGACGAGTAC27940.7691990034000028No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTAGTGCTAAAATGGAGATTC27460.755984417801148No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA25400.6992718212727298No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT25060.6899114898068743No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC23970.6599033683428084No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT23860.6568750258097376No Hit
GTAGAGGAGCCGTCAAATCCACACTGACGACATGGTTCTACAGTAGAGGA23120.6365025396781697No Hit
TGTGGTCTGCCAGCTAAAGGGTTTGTACTTTACTTTCATTGGGAGAAGAC22540.620534915412887No Hit
ACTTCTACCGTGCCCTGCTACCGTAAGCTGTGTCTACTCATTTGAACAGA22100.6084215452806034No Hit
GCAACAGATAACTCAGATTGCCTTCAACAGATAACTCAGATTGCCTTCAA21920.6034660756810329No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG21520.5924539210153207No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG21240.5847454127493221No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG19850.5464781752859719No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT19580.5390449708866162No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG18790.5172959654218343No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC17160.4724214351590568No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC16930.46608944622627224No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG16180.4454416562280617No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGATCGTCTCTATCTCG14890.4099274574311396No Hit
CAGAGGGGAAAAATATGACAAAGAGCAGGGTGCTAAAATGGAGATTCTCT14530.4000165182319986No Hit
CAGAGGGGAAAAATATGACAAAGGCACTAGTGTGCTAAAATGGAGATTCT14510.39946591049871294No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT14480.39863999889878454No Hit
GTAGAGGAGCCGTCAAGAGGGATAAAACACCATGAAAGACCAAGTCTCTG14380.3958869602323565No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA13700.3771662973006456No Hit
GGGGTCAGAGGCAAGCAGAAACACATGGGAGATAACACAGGCCCAAGAGA13270.3653282310350049No Hit
TCAAATCATCCATTGCTTGGCGGTAGCAGAAAGTCAGTCCCATGGAGACC12410.34165209850372347No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT12350.3400002753038667No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC11940.32871281677151154No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCAAGGTGGGGGCAGCGCCT11090.305311988106873No Hit
TGTGCTGTGACTGCTTGTAGAACCAACAGAGTTGAAAGTCAGGGCAAGAC10890.29980591077401686No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG10120.2786075130425207No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA9900.2725508279763789No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACACGGACGACGTGGTGGAT9620.26484231971038036No Hit
GGGGTCAGAGGCAAGCAGGCTGGAGATAACACAGGCTGGAGATAACACAG9340.25713381144438174No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT9150.25190303797816843No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGAT8810.24254270651231294No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT8490.23373298277974314No Hit
CAGCCTCTGGCATTCTGGATCAGAGCAGTCAGAGGACCAGGAGACCAAGT8360.23015403251338665No Hit
GGGGTCAGAGGCAAGCAGGAATTCCGCGGAGATAACACAGGCCCAAGAGA8310.22877751318017264No Hit
GGGGTCAGAGGCAAGCAGGATACACGCAGACAGGGAGATAACACAGGCCC8010.22051839718088842No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCGTAGGGACTCTG7930.21831596624774596No Hit
GCAGCTTCTGCCATCTCTCTGCTACAGAACGTGGGAGTAGACGGAAGACC7890.21721475078117472No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGATCGTC7610.20950624251517613No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCCGCGCTAATGAGTTTGT7430.2045507729156056No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTATCATTCACTCCTGGCCTTGG7240.19931999944939227No Hit
GCAACAGATAACTCAGATTGCCTTCAACAGATAACTCATACCAGGACCAG6840.18830784478367998No Hit
AGCTGCTCACCATCGCTAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCT6380.17564386691811087No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA6210.17096370118518314No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA6140.16903657411868347No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAGAACGTGGGAGTAGACGGAAG6110.16821066251875508No Hit
TGACAGTTTGACAGTTAAAGGCATGTAGAACCCATGTTCAGCCGTTACCT5990.1649070161190414No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC5510.15169243052018666No Hit
TGACAGTTTGACAGTTAAAGGCATACACTGACGACATGGTTCTACATGAC5490.15114182278690103No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC5240.14425922612083086No Hit
CGAGCAAGAGGCTTTGGAGTACACTGACGACATGGTTCTACTCTCAGCAG5120.1409555797211172No Hit
TCTGTATCAGGCAAAGTCATAGAACCAGGAGCCATTGTCTTTGAGGCAGA4920.13544950238826103No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGATCGTCTCTAT4600.1266397786556912No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGTCGTCAGAGGGACGAACTGG4590.12636447478904841No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT4590.12636447478904841No Hit
GGGGTCAGAGGCAAGCAGGGTTCTCTGATGAGCTCAGGGACACAGGAGAT4560.12553856318912No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG4480.12333613225597755No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA4430.1219596129227635No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA4330.11920657425633543No Hit
GGTCTTCTCTGTTTCAGGGCATTCTCCTGGAGGTGGCTTTAGGTCAGCAG4070.11204867372362246No Hit
GTAGAGGAGCCGTCAAATCCACACTGACGACATGGTTCTACAGTAGAGGG4060.11177336985697965No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC4050.11149806599033683No Hit
ATACGGCCAGGCATTGAAGTTACACTGACGACAGGGGCCAGGAGGAGACC3950.10874502732390876No Hit
TCTTAAATGGCTACGACCCAGACCAAGTGCTACCTGTTAAAGAATCATCT3910.10764381185733754No Hit
GGAATCCTATGGCTTTCCAACCTACAGCAACAGAGGAGGGGGAGACCAAG3810.10489077319090948No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT3760.10351425385769543No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT3760.10351425385769543No Hit
AGCTGCTCACCATCGCTACCGCAGGTCTTGGCCAGTTGGAGACCAAGTCT3670.10103651905791015No Hit
TCCCTGCCAGCGAGATGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTG3670.10103651905791015No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCCGTCATAGGGACTCTG3660.10076121519126736No Hit
CAGAGGGGAAAAATATGACAAGTCTCTGCTACCGTAGTGCTAAAATGGAG3650.10048591132462456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)