FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008104495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008104495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363235
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA293108.069156331300672No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA240026.6078434071606535No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG100742.7734111525596377No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG81142.233815573939736No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG73782.03119192809063No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA73702.0289894971574873No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA70051.928503585832863No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC69611.9163902157005794No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTAATTACCCAAAAGTGAAAC63941.760292923314108No Hit
AAGGGTGCAGTTATGCCTCAAATCGCTCCAGATATCCACATGCAGATACA53541.4739769020055888No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA52601.4480983385411648No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA51401.415061874544028No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT50711.3960659077456743No Hit
TCATTACACCAGTTCGTCCCTACCGTGGTAAAAAGATAACCTCAGAATAA48531.3360496648175424No Hit
TTCATGGTGTTTTATCCCTCTTGACGGCTCCTCTACTGTAGAACCATGTC47721.3137500516194751No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA44841.2344625380263465No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT43691.2028025933624238No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT42901.181053587897642No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA40631.1185596101697248No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG37381.0290858535108127No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC36220.9971506049802469No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGTCGTC34660.954203201783969No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT34360.9459440857846848No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT32390.8917092240560519No Hit
TCCAGGAAGAGGAAAGGAAAAACAAGTCGTCAGTGTTCATCTTCACTTAG32160.8853772351232673No Hit
GTTTCCTCTGGTCCTGGTATGAGTTATCTGTTGAAGGCAATCTGAGTTAT31570.8691343069913418No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT30140.8297658540614202No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA29250.8052638099302104No Hit
AACAGAGAATCTCCATTTTAGCACTAGCAGAGACTTTGTCATATTTTTCC27920.7686483956667172No Hit
ATGAGGTACTCGTCGGCATCCATAGGTAGCAGAGACTTGGCTATAGGAGC26890.740292097402508No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC26480.7290046388701529No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT25540.703126075405729No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA24030.6615551915426652No Hit
GTTCAAATGAGTAGACACAGCTTGGTCTTCTCTGTTTCAGGGCACGGTAG23930.6588021528762372No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTAGCTGGCAGACCACATGTA22670.6241138656792435No Hit
GTTCAAATGAGTAGACACAGCTTACGGTAGCAGGGCACGGTAGAAGTTGT22230.6120004955469599No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA21920.6034660756810329No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG21300.5863972359491789No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT19750.5437251366195438No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG19560.5384943631533304No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG18300.5038060759563369No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT16940.46636475009291506No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG16930.46608944622627224No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC16120.4437898330282049No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG15840.43608132476220623No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC15340.42231613143006597Illumina Single End PCR Primer 1 (100% over 21bp)
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG15180.417911269563781No Hit
AACAGAGAATCTCCATTTTAGCACACTAGTGCCTTTGTCATATTTTTCCC14650.4033201646317122No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA14650.4033201646317122No Hit
AACAGAGAATCTCCATTTTAGCACCCTGCTCTTTGTCATATTTTTCCCCT14510.39946591049871294No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG13650.37578977796743157No Hit
CTTGGGCCTGTGTTATCTCCCATGTGTTTCTGCTTGCCTCTGACCCCTGT13160.362299888501934No Hit
CCATGGGACTGACTTTCTGCTACCGCCAAGCAATGGATGATTTGATGTAG12580.3463322642366512No Hit
GGCCTCAGTACAAACTCATTAGCTAGCAGAGACTTGGTCTGGACCTAATT12260.33752254050408137No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA11610.31962778917229895No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC11250.30971684997315785No Hit
TGCCCTGACTTTCAACTCTGTTGGTTCTACAAGCAGTCACAGCACATGTA10880.29953060690737404No Hit
CTTGGGCCTGTGTTATCTCCAGCCTGTGTTATCTCCAGCCTGTGTTATCT9760.26869657384337964No Hit
AACAGAGAATCTCCATTTTAGCACCAGAGACTTTGTCATATTTTTCCCCT9360.2576844191776674No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA9180.25272894957809683No Hit
CACACACAGGTAACGGCTGAACATGGGTTCTACATGCCTTTAACTGTCAA9000.24777347997852628No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA8910.24529574517874103No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT8710.2397896678458849No Hit
CCTGGTCCTCTGACTGCTCTGATCCAGAATGCCAGAGGCTGTGTAGAACC8450.23263176731317192No Hit
CTTGGGCCTGTGTTATCTCCCTGTCTGCGTGTATCCTGCTTGCCTCTGAC8230.22657508224703016No Hit
CTTGGGCCTGTGTTATCTCCGCGGAATTCCTGCTTGCCTCTGACCCCTGT8220.22629977838038734No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC8170.2249232590471733No Hit
TCCGTCTACTCCCACGTTCTGTAGCAGAGAGATGGCAGAAGCTGCTGTAG8020.2207937010475312No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC7540.2075791154486765No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT7420.20427546904896282No Hit
GGCCTCAGTACAAACTCATTAGCGCGGAGACTTGGTCTGGACCTAATTAC7370.20289894971574876No Hit
ATGAAAACACAACATGAATATAAACATCAATGCTAAAAACCAAGGCCAGG7370.20289894971574876No Hit
GGTCTTTGCCTGCTGAGAGTAGAACCATGTCGTCAGTGTACTCCAAAGCC7360.20262364584910597No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC6870.1891337563836084No Hit
CCAACTGGCCAAGACCTAGCGATGGTGAGCAGCTTGTAGAACCATGTCGT6390.17591917078475366No Hit
TCCGTCTACTCCCACGTTCTACGGTAGCAGAGAGATGGCAGAAGCTGCTG6150.16931187798532632No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG5270.1450851377207593No Hit
GCCTCAAAGACAATGGCTCCTGGTTCTATGACTTTGCCTGATACAGATGT5030.13847784492133192No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA4930.13572480625490385No Hit
CTTGGGCCTGTGTTATCTCCTGTGTCCCTGAGCTCATCAGAGAACCCTGC4890.1346235907883326No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4630.12746569025561963No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT4630.12746569025561963No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC4550.1252632593224772No Hit
TCATTACACCAGTTCGTCCCTCTGACGACGTGGTAAAAAGATAACCTCAG4520.12443734772254876No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT4440.1222349167894063No Hit
TGCCCTGACTTTCAACTCTGTTTCCTTCCTCTTCCTACAGTACTCCCCTG4430.1219596129227635No Hit
AGGGATGCAAAGGCCTCATCATCTCGCTGGCAGGGATGTAGAACCATGTC4180.11507701625669332No Hit
GCTGACCTAAAGCCACCTCCAGGAGAATGCCCTGAAACAGAGAAGACCTG4140.1139758007901221No Hit
CCCCCTCCTCTGTTGCTGTAGGTTGGAAAGCCATAGGATTCCTGTAGAAC3950.10874502732390876No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT3910.10764381185733754No Hit
CCTCCTGGCCCCTGTCGTCAGTGTAACTTCAATGCCTGGCCGTATTGTAG3900.10736850799069474No Hit
TCCAGATGATTCTTTAACAGGTAGCACTTGGTCTGGGTCGTAGCCATTTA3790.10434016545762385No Hit
CCAACTGGCCAAGACCTGCGGTAGCGATGGTGAGCAGCTTGTAGAACCAT3720.1024130383911242No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTGTCATATTT3710.10213773452448141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)